Control of mildew in vines with cinnamon extract and catalase activity in organic production

Authors

DOI:

https://doi.org/10.33448/rsd-v10i10.18885

Keywords:

Cinnamomum zeylanicum; Enzymes; Plant extracts; Plasmopara viticola.

Abstract

Management with synthetic fungicides in the control of phytopathogens in viticulture can cause environmental pollution and contamination with residues in grape cluster. The objective of this study was to verify the effect of aqueous cinnamon extract on the in vitro and in vivo control of Plasmopara viticola on catalase activity on ‘Isabel Precoce’ vines. The treatments used were: aqueous cinnamon extract (ACE) at concentrations of 0.12; 0.25 and 0.50% plus 0.25% vegetable oil (VO); being the standard treatments VO (0.25%), Bordeaux mixture 1:1:100 (lime: copper sulfate: water) and water only. The germination tests of P. viticola sporangia were carried out in incubation periods of 4 and 24 hours of the pathogen in contact with the treatments. In addition, the area under the disease progress curve (AUDPC) and the activity of the catalase enzyme were estimated in plants grown in the greenhouse. The results indicated that the treatments with 0.12%, 0.25% and 0.5% ACE with VO reduced the germination of P. viticola. In relation to the AUDPC, the 0.25% dose of VO associated ACE reduced 65% and 67% in leaf discs and vines in the greenhouse, respectively. This fact is related to the induction of CAT activity provided by this dose in the periods of 2HBA, 2HAI and 4HAI. Thus, it can be said that the ACE associated with VO can be used to control the downy mildew of the ‘Isabel Precoce’ vine.

References

Azevedo, L. A. S. Manual de quantificação de doenças de plantas. Novartis, 1997. 114p.

Bogorni, P. C. & Vendramim, J. D. (2003). Bioatividade de Extratos Aquosos de Trichilia spp. sobre Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) em Milho. Neotropical Entomology. http://www.scielo.br/pdf/%0D/ne/v32n4/a18v32n4.pdf

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72 (1-2), 248-254. https://doi.org/10.1016/0003-2697(76)90527-3

Brugalli, I. (2003). Alimentação alternativa: a utilização de fitoterápicos ou nutracêuticos como moduladores da imunidade e desempenho animal. In: Simpósio Sobre Manejo e Nutrição de Aves e Suínos, 2003, Campinas. Anais: CBNA. 167-182.

Casali, C. A., Moterle, D. F., Rheinheimer, D. S., Brunetto, G., Corcini, A. L. M., Kaminski, J. & Melo, G. W. B. 2008. Formas e dessorção de cobre em solos cultivados com videira na Serra Gaúcha do Rio Grande do Sul. R Bras Ci Solo, 32(4), 1479-1487, http://dx.doi.org/10.1590/S0100-06832008000400012.

Dagostin, S., Schärer, H. J., Pertot, I. & Tamm, L. (2011). Are there alternatives to copper for controlling grapevine downy mildew in organic viticulture? Crop Protection, 30(1), 776-788. https://doi.org/10.1016/j.cropro.2011.02.031

Ferreira, D. F. (2011). SISVAR: a computer statistical analysis system. Ciência e Agrotecnologia, 35(6), 1039-1042. http://ref.scielo.org/2cwvvs

Flávio, N. S. D. S., Sales, N. L. P., Aquino, C. F., Soares, E. P. S., Aquino, L. F. S. & Catão, H. C. R. M. (2014). Qualidade sanitária e fisiológica de sementes de sorgo tratadas com extratos aquosos e óleos essenciais. Semina: Ci Agr, 35(1), 7-20. http://www.uel.br/revistas/uel/index.php/semagrarias/article/viewFile/9602/14047

Garcia, C., Faria, C. M. D. R., Santa, H. S. D., Botelho, R. V., & Maia, A. J. (2018). Antimicrobial activity of Agaricus brasiliensis on Plasmopara viticola and its effect on the induction of resistance to the control of downy mildew on ‘Isabel Precoce’. Arquivos do Instituto Biológico, 85. https://doi.org/10.1590/1808-1657000052017

Garcia, C., Horst, M. V., Maia, A. J., Leite, C. D., Faria, C. M. D. R. & Schwan-Estrada, K. R. F. (2017). Óleo essencial de alecrim no controle de doenças da videira cultivar Cabernet Sauvignon. R Brasileira Ciências Agrárias, 12(4), 452-457. http://www.redalyc.org/pdf/1190/119054185010.pdf

Gessler, C., Pertot, I. & Perazzolli, M. (2011). Plasmopara viticola: a review of knowledge on downy mildew of grapevine and effective disease management. Phytopathol Mediterr, 50 (1), 3-44. http://dx.doi.org/10.14601/Phytopathol_Mediterr-9360

Góth, L. A. (1991). A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta, 196(2), 143-151. https://doi.org/10.1016/0009-8981(91)90067-M

Khan, M. S. A. & Ahmad, I. (2011). In vitro antifungal, anti-elastase and anti-keratinase activity of essential oils of Cinnamomum-, Syzygiumand- and Cymbopogon-species against Aspergillus fumigatus and Trichophyton rubrum. Phytomedicine, 19(1), 48-55. https://doi.org/10.1016/j.phymed.2011.07.005

Kumar, A., Shukla, R., Singh, P. & Dubey, N. K. (2009). Biodeterioration of some herbal raw materials by storage fungi and aflatoxin and assessment of Cymbopogon flexuosus essential oil and its components as antifungal. Int Biodeterior Biodegradation, 63(6), 712-716. https://doi.org/10.1007/s00248-008-9375-x

Mittler, R. (2017). ROS are good. Trends plant Sci, 22(1), 11-19. https://doi.org/10.1016/j.tplants.2016.08.002

Pons, A., Mouakka, N., Deliere, L., Crachereau, J. C., Davidou, L., Sauris, P., Guilbault, P., Darriet, P. (2018). Impact of Plasmopara viticola infection of Merlot and Cabernet Sauvignon grapes on wine composition and flavor. Food Chem, 239(1), 102-110, 2018. https://doi.org/10.1016/j.foodchem.2017.06.087

Pinto, K. M. S., Nascimento, L. C., Oliveira, A. K., Leite, R. P. & Silva, J. P. (2013). Resistência induzida em frutos de videira ‘Isabel’ (Vitis labrusca) e seus efeitos sobre a qualidade pós-colheita. Revista Brasileira de Fruticultura, 35(1), 210-217. http://dx.doi.org/10.1590/S0100-29452013000100024

Quan, L. J., Zhang, B., Shi, W. W. & Li, H. Y. (2008). Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. J Integr Plant Biol, 50(1), 2-18. https://doi.org/10.1111/j.1744-7909.2007.00599.x

Shuping, D. S. S. & Eloff, J. N. (2017). The use of plants to protect plants and food against fungal pathogens: A review. Afr J Tradit Complement Altern Med, 14(4), 120-127. 10.21010 / ajtcam.v14i4.14

Venturoso, L. R., Bacchi, L. M. A., Gavassoni, W. L., Conus, L. A., Pontim, B. C. A. & Souza, F. R. (2011). Inibição do crescimento in vitro de fitopatógenos sob diferentes concentrações de extratos de plantas medicinais. Arquivos Instituto Biológico, 78(1), 89-95. http://www.biologico.sp.gov.br/uploads/docs/arq/v78_1/venturoso.pdf

Zyl S. A. V., Brink, J. C., Calitz, F. J. & Fourie, P. H. (2010). Effects of adjuvants on deposition efficiency of fenhexamid sprays applied to Chardonnay grapevine foliage. Crop Protection, 29(1), 843-852. https://doi.org/10.1016/j.cropro.2010.04.017

Downloads

Published

08/08/2021

How to Cite

GARCIA, C.; FEDRIGO, K. .; GABRIEL, A.; BOTELHO, R. V. .; RODRIGUES, J. D.; ONO, E. O. . Control of mildew in vines with cinnamon extract and catalase activity in organic production. Research, Society and Development, [S. l.], v. 10, n. 10, p. e214101018885, 2021. DOI: 10.33448/rsd-v10i10.18885. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/18885. Acesso em: 18 apr. 2024.

Issue

Section

Agrarian and Biological Sciences