Evaluation preliminary phytochemistry and antimicrobial activity of extracts in Eugenia uniflora L. on Escherichia coli and Staphylococcus aureus

Authors

DOI:

https://doi.org/10.33448/rsd-v10i13.20579

Keywords:

Bacterial activity; Natural products; Microorganisms.

Abstract

Devido ao aumento da resistência de patógenos humanos e animais aos medicamentos atuais, pesquisas por novos antimicrobianos têm sido incentivadas, principalmente por meio de plantas medicinais. Eugenia uniflora L., conhecida como pitangueira, é popularmente utilizada com diversas funções terapêuticas, justificando estudos nesta espécie. Assim, os objetivos deste estudo foram avaliar a atividade antibacteriana “in vitro” de extratos de folhas e cascas de Eugenia uniflora L. sobre Escherichia coli e Staphylococcus aureus. Para as etapas de triagem fitoquímica e microbiologia, foram utilizadas as metodologias usuais nessas áreas para obtenção dos extratos e avaliação da atividade antibacteriana com uso de controle positivo e negativo. Os resultados obtidos mostraram que no extrato das folhas foi verificada a presença de saponinas, flavonóides, alcalóides e taninos, enquanto na casca foram obtidos apenas saponinas e alcalóides. Os extratos da folha e da casca foram capazes de inibir o crescimento de Stapylococcus aureus apenas, promovendo halos de inibição de meio de 19,4 mm e 14,4 mm, respectivamente. As análises permitiram concluir que os extratos apresentam metabólitos secundários interessantes e de grande potencial antimicrobiano e que esses extratos foram capazes de inibir apenas as cepas de S. aureus, sendo resistentes às cepas de E. Coli.

References

Albuquerque, F. H. C., Soares, K. S., & Oliveira, M. A. S. (2017) In vitro antimicrobial activity of aqueous, hydroalcoholic and alcoholic extracts from the leaves of species of the Myrtaceae family prior to the strains of bacteria of interest. J. Med. Biol. Sci. Salvador. 16: 139-45.

Abrantes J. A. (2017) Antimicrobial evaluation and photochemical prospecting for Eugenia florida DC. Dissertation (Post-Graduation) - Institute of Technology in Pharmaceuticals, Rio de Janeiro-RJ.

Almeida, M. Z. (2011) Medicinal plants. (3a ed.), EDUFBA, 221 p

Alvarenda, F. Q, Royo, V. A., & Mota, B. F. C., Laurentiz R.S., Menezes E.V, Melo Junior A.F, et.al.(2015) The Antinociceptive and Antimicrobial Activity of the Stalk Bark Psidium Cattleyanum Sabine. Braz. J. Med. Plants, 17: 1125-33.

Auricchio, M. T., & Bacchi, E. M. (2003) Leaves of Eugenia uniflora L. (pitanga): pharmacobotanical, chemical and pharmacological properties. Rev. Inst. Adolfo Lutz 62: 55-61.

Ayres M. C. C, Brandão M. S, Vieira-Júnior G. M, Menor J. C. A. S., Silva H. B, Soares M. J. S, et.al. (2008) Antibacterial activity of useful plants and chemical constituents of the root of Coperniciaprunifera. Braz. J.Pharmacognosy, 18: 90-97.

Bell V. (2014) Introduction of antibiotics in Portugal: science, technique and society (40s to 60s of the twentieth century). Penicillin case study. Thesis (Doctorate in Pharmaceutical Science) - University of Coimbra, São Paulo-SP.

Bezerra W. G. A., Horn R. H., Silva I. N. G., Teixeira R. S. C, Lopes E. S., Albuquerque, Á. H.,. et.al. (2016) Antibiotics in the poultry sector: a review on microbial resistance. Arch. Zootec. Fortaleza. 66: 287- 302.

Bouzada M. L. M., Fabria R. L., Duarte G. G. & Scio E. (2009) Search for new antimicrobial drugs from vegetables. Principia, Juiz de Fora. 11: 1-8.

Brandelli C. L. C, Giordani R. B; Carli G. A. & Tasca T. (2009) Indigenous traditional medicine: in vitro anti-giardial activity of plants used in the treatment of diarrhea. Parasitol. Res., Berlin. 104: 1345–49.

Brazil.(2018) Ministry of Health. Secretariat of Health Surveillance. Department of Surveillance of Communicable Diseases.. National action plan for the prevention and control of antimicrobial resistance in the context of single health 2018-2022 (PAN-BR) / Ministry of Health, Secretariat of Health Surveillance, Department of Surveillance of Communicable Diseases. Brazil: Ministry of Health.

Brazil. (2014) Resolution of the Collegiate Board - RDC N ° 26, of May 13, Provides for the registration of herbal medicines and the registration and notification of traditional herbal products. Available at: http: //bvsms.saude.gov.br/bvs/saudelegis/anvisa/2014/rdc0026_13_05_2014.pdf. Accessed on: September 20, 2019

Castro H. G. (2004) et al. Contribution to the study of medicinal plants: secondary metabolites. (2a ed.), UFV, 113p.ç

Castro L. L. D., Sprenger L. K., Madrid I. M., Oliveira F. C., Oliveira P. A., Castro L. M., et.al (2019). In vitro effect of extracts of Eugenia uniflora L. on gastrointestinal nematodes in sheep. Brazilian An. Sci. Goiânia. 20: 1-12.

Cavalcante R. (2019) Plants in dentistry: a practical guide. (3a ed.) Managed

CLSI. (2012) Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing: Informational supplemente M100-S22.Wayne, Pa: CLSI

Dias M. T., Santos, P. C. R. F. Oliveira L. A. T. & Marin V. A. (2010) Evaluation of the sensitivity of Escherichia coli strains to antimicrobials. Food Sci. Technol. 30: 319-24.

Duarte M. C. T (2006) Antimicrobial activity of medicinal and aromatic plants used in Brazil. Rev. Multiciência, 2006; 7: 1-16.

Ferreira F. P. S. (2014) Leaf morphology, phytochemistry and antimicrobial activity of Eugenia calycina Cambess. (Myrtaceae) Dissertation (Post-Graduation in Pharmaceutical Sciences) - Federal University of Goiás, Goiânia.

Fiuza T. S., Rezende M. H., Sabóia-Morais S. M. T. Bara M. T. F. & Tresvenzol L. M. F, Paula J. R. (2008) Pharmacognostic characterization of Eugenia uniflora L. (Myrtaceae) leaves. Rev. Eletronica Farm. Goiânia 5: 21-32.

Fracarolli I. F. L., Oliveira S. A, & Marziale M. H. (2017) Bacterial colonization and antimicrobial resistance in health workers: an integrative review. Act. Paul. Enfer. 30: 651-57.

Gobbo-Neto L. & Lopes, N. P. (2007) Medicinal plants: factors influencing the content of secondary metabolites. Quím. Nova, 30: 374-81.

Lameira O. A., Paiva J. S. & Oliveira E. C. P., Pinto J. E. P. (2003) Phenology and Phytochemical Analysis of Medicinal Plants Occurring in the Amazon. http: // www. ab.horticultura. with. br / library / files / Download / Library / 44_701.pdf.

Leite T. C. C., Santos R. H., Edge E F. O, Chargas E. C. O, Sena & A. R; Silva T. G. (2014) Evaluation of the antimicrobial activity of Miconia amacurensiswurdack. In: North Northeast Congress of Research and Innovation, São Luís. Anais IX CONNEPI, Federal Institute of Education, Science and Technology in Maranhão,

Lima R. A., Silva, A. C. O. (2016) .Identification of the classes of secondary metabolites in the ethanolic extract of the fruits and leaves of Eugenia uniflora L. 2016. Rev. electr. Manag. Educ. and Environm. Technol. 20: 381-88.

Lopes M. M. (2008) Chemical composition, antimicrobial and allelopathic activity of the essential oils of Eugenia uniflora L. and Myrciariaglazioviana (kiaersk) GM Barroso and Sobral (Myrtaceae). Dissertation (Master in Microbiology) - Federal University of Viçosa-MG.

Lorenzoni L. S., Gandini S. M. S., Souza, T. S., Junior A. C. S. & Ulisses A. F. (2013) Phytochemical and antibacterial study of the ethanolic extract of Eugenia uniflora L. (Myrtaceae). Encyclopedia biosphere, Goiânia. 9: 279-96.

Lorenzoni L.S; Bernardo, Fpamoulin T., Gandine S. M. S; Souza, T. S.(2011) Use of crude extracts of Eugenia uniflora L. (Pitanga) in antibacterial tests in vitro. In: Latin American Scientific Initiation Meeting, São José dos Campos-SP. Proceedings of the xv Latin American Scientific Initiation Meeting, XI Latin American Post-Graduation Meeting at Univap

Madigan M. T, Martinko J. M, Bander K. S, Buckley & D. H, Stahl D. (2016) Microbiology, (14a ed.), Artemed, 1032p.

Martinez A. M. (2005) FLAVONÓIDES. Faculty of Pharmaceutical Chemistry, University of Antioquia. https: //www.yumpu. com / es / document / view / 14473174 / flavonoids -facultad-de-quimica-pharmaceutica-universidad-.

Martins F. M. M. (2012) Study of the influence of environmental factors on the chemical composition and biological activities of Xylopia sericea.. Dissertation (Master in Tropical Biodiversity) - Federal University of Espírito Santo, São Mateus-ES.

Michelin D. C., Moreschi A. C., Lima G. G. F., Nascimento M. O & Chaud, M. V. (2005) Evaluation of the antimicrobial activity of plant extracts. Braz. J.Pharmacognosy, 5: 316-20.

Migliato K. F, Corrêa M. A., I. Salgado, H. R. N. Tognolli J. O., Sacramento L. V. S, Mello J. C. P, et.al. (2011) Experimental planning to optimize the extraction of the fruits of Syzygiumcumini (L.) Skeels. Quim.Nova, São Paulo. 34: 695-99.

Monteiro J. M. Albuquerque U. P., Araújo L. E. &Amorim E. L. C. (2005) Tannins: an approach from chemistry to ecology. Quim. Nova, 28: 892-96.

Oliveira A. C, Paula, A., & Rocha R. F (2015) Costs with antimicrobials in the treatment of patients with infection. research Article, Minas Gerais.; 33: 352-61.

Oliveira C. B., Soares D. G. S., Paulo M. Q. & Padilha W. W. N. (2008) In vitro antimicrobial activity of Eugenia uniflora L. (Pitanga) on cariogenic bacteria. Braz. Jour.of Health Sciences, João Pessoa; 12: 239-50.

O'neill J. (2016) Tackling Drug-resistant infections Globally: final report and recommendations. Rev. Antimicrobial Resist., United Kingdom, 76p.

Pereira J. F. S. (2007) Evaluation of the antimicrobial activity of the antimicrobial potential of the schinopsi brasiliensis Engler bark extract: a study based on the etapharmacological indication. Monograph (Graduation in Pharmacy) - State University of Paraíba, Campina grande- PB.

Rodrigues A. G., Santos M. G., Amaral A. C. F. (2006) Public policies on medicinal plants and herbal medicines. In: Ministry of Health (org) Phytotherapy in SUS and the research program for medicinal plants at the central of medicines.1st edition. Brasília: Ministry of Health,

Rodrigues L. S., Silva A. R. A. & Macedo A. A. M. (2017) Noni (MorindacitrifoliaLinn.) Phytochemical determination and antioxidant potential by the DPPH method. Scie. Techn. Connec. 11: 47-54.

Rozatto M. R. (2012) Determination of in vitro antimicrobial activity of extracts, fractions and compounds isolated from Arrabidaea brachypoda. . Dissertation (Postgraduate studies in Pharmaceutical Sciences) - Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara-SP.

Saad G.A, Leda P.H.O, S.Á. IM. & Seixlack A.C.C. (2016) Contemporary herbal medicine: tradition and science in clinical practice. (2a ed.), Guanabara Koogan,

Santos DYAC. Applied botany: secondary metabolites in plant-environment interaction. 2015.Universidade de São Paulo, São Paulo, 2015. <http://www.tese.usp.br/tese/disponiveis/vredocencia/41/tde-29092015-103721/>

Santos O. B. (2008) Production of herbal herbal extracts. Managed. 78p.

Scur M.C., Pinto F.G.S., Pierozan F.D., Arruda G. & Zampronio E. (2014) The antioxidant activity and phytochemical prospecting of plant extracts from Eugenia uniflora. In: National Congress of Research in Applied Social Sciences, 2014. Annals III CONAPE, Francisco Beltrão: Conape, .1- 4.

Silva A.C.& Lima R.A. (2016) Identification of the classes of secondary metabolites in the ethanolic extract of Eugenia unifloraL fruits and leaves Electronic journal on management education and environmental technology. 20: 381-88.

Silva, N.C.C. (2010) Comparative study of the antimicrobial action of extracts and essential oils from medicinal plants and synergisms with antimicrobial drugs. . Monograph (Graduation in General Biology) - Universidade Estadual da Paulista, Botucatu-SP.

Silveira M.S.S, Olea R.S.G., Mesquitas J.S., Cruz A.L.N. & Mendes J.C. (2009) Methodologies of antimicrobial activity applied to plant extracts: comparison between two diffusion agar techniques. Braz. J.Pharmacognosy. 90: 124-128.

Tavares J. C. (2018) Medicinal plants: use, guidelines and precautions. (3a ed.), Thieme Revinter Publicações,

Tomás-Menor L, Barrajón-Catalán E, Segura-Carretero A, Martí N. Saura D. & Menéndez J.A, et.al. (2015) The Promiscuous and Synergic Molecular Interaction of Polyphenols in Bactericidal Activity: An Opportunity to Improve the Performance of Antibiotics? Phytother. Res.; 29: 466–73.

Tomita L. K. (2014) Phytochemical characterization and biological assays of Syngonium podophyllum. Monograph (Graduation in Pharmacy-Biochemistry) - Universidade Estadual Paulista, Araraquara-SP.

Tortora G. J., Funke B. R., Case C. L.(2017) Microbiology. (12a ed.), Artmed.

Trugilo P. F., Moni F. A., Lima J. T. & Cardoso D. P. (2003) Determination of the tannin content in the bark of Eucalyptus spp, Cerne, Lavras. 9: 246-54.

Vizzoto M. Krolow A. C, Weber G. E. B. (2010) Secondary metabolites found in plants and their importance. 1st edition, Pelotas-RS: Embrapa ClimaTemperado, 16p.

World Health Organization - WHO. (2015) Global Antimicrobial Resistance Surveillance System: Manual for Early Implementation. Geneva: WHO.

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Published

20/10/2021

How to Cite

BITTENCOURT, A. H. C. .; MACHADO, J. S. .; SILVA, M. G. da .; COSENZA, B. A. P. . Evaluation preliminary phytochemistry and antimicrobial activity of extracts in Eugenia uniflora L. on Escherichia coli and Staphylococcus aureus. Research, Society and Development, [S. l.], v. 10, n. 13, p. e329101320579, 2021. DOI: 10.33448/rsd-v10i13.20579. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/20579. Acesso em: 18 apr. 2024.

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Section

Agrarian and Biological Sciences