Influence of the extraction process on the antioxidant capacity of pequi pulp extracts

Flávia de Abreu  Pinheiro; Leonardo Luiz  Okumura; Astréa Filomena de Souza  Silva; Júnio Gonçalves da  Silva; Deusélio Bassini  Fioresi; Wilton Soares  Cardoso; Letícia Rocha  Ferreira; Edimar Aparecida Filomeno Fontes

doi:10.33448/rsd-v10i12.20663

Autores

Flávia de Abreu Pinheiro Federal Institute of Espírito Santo https://orcid.org/0000-0002-4310-0592
Leonardo Luiz Okumura Federal University of Vicosa https://orcid.org/0000-0002-3056-2966
Astréa Filomena de Souza Silva Federal University of Vicosa https://orcid.org/0000-0003-1992-7968
Júnio Gonçalves da Silva Federal University of Minas Gerais https://orcid.org/0000-0002-3714-3633
Deusélio Bassini Fioresi Federal Institute of Espírito Santo https://orcid.org/0000-0002-0912-0813
Wilton Soares Cardoso Federal Institute of Espírito Santo https://orcid.org/0000-0001-8067-6921
Letícia Rocha Ferreira Federal University of Vicosa https://orcid.org/0000-0003-0983-5584
Edimar Aparecida Filomeno Fontes Federal University of Vicosa https://orcid.org/0000-0002-4370-8805

DOI:

https://doi.org/10.33448/rsd-v10i12.20663

Palavras-chave:

Solvente; Composto bioativo; Análise voltamétrica; Ensaio eletroquímico; Voltametria de pulso diferencial; Análise de componente principal.

Resumo

Foi investigado o efeito do procedimento de extração na capacidade antioxidante de extratos da polpa de pequi: aquoso (AQ), alcoólico (ALC) e acetometílico (AM), obtidos respectivamente pelos solventes água, etanol e metanol seguido de acetona. A ação antioxidante foi expressa pelo coeficiente de capacidade antioxidante (K) obtido pelo teste eletroquímico (DPV) em diferentes valores de pH, e em equivalente ao trolox (TEAC), por DPV (pH 7,15) e ensaios espectrofotométricos ABTS e DPPH. Os teores de carotenoides e fenólicos totais também foram determinados. Por meio das análises de Componentes Principais e Cluster, observou-se maior similaridade de K entre AM, trolox e ácido gálico, AQ e ácido L-ascórbico, e ALC e β-caroteno, independentemente do pH. Por meio da análise de cluster, a maior diferenciação de K foi em pH 2,20. Em geral, o AM apresentou melhor ação antioxidante (TEAC). AQ e ALC apresentaram os maiores teores de fenólicos e carotenoides, respectivamente. O método de extração influenciou o teor de compostos bioativos dos extratos de pequi e, portanto, sua capacidade antioxidante.

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Influência do processo de extração na capacidade antioxidante de extratos de polpa de pequi

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