Incorporação de bactérias probióticas no cultivo de larvas de Macrobrachium amazonicum (Heller, 1862)

Autores

DOI:

https://doi.org/10.33448/rsd-v10i14.21721

Palavras-chave:

Camarão-da-amazônia; Larvicultura; Bactéria ácido lática.

Resumo

Neste estudo avaliou-se o uso de dois probióticos na larvicultura do camarão-da-amazônia. Foram testados a bactéria ácido láctica autóctone Enterococcus faecalis, isoladas a partir do tubo digestório de juvenis de M. amazonicum selvagens e um probiótico comercial. As cepas probioticas foram diluídas diretamente na água e homogeneizada na ração fresca, na concentração de 1,85 x106 UFC/ ml e 1,85 x106UFC/g, respectivamente. A inoculação na água ocorreu apenas no início do experimento, enquanto que na dieta, foi adicionado a partir do décimo dia em diante, diariamente. Parâmetros como: sobrevivência, produtividade, peso fresco, comprimento, índice de condição larval (ICL) e índice de estágio larval (IEL) foram avaliados. Ocorreu a colonização do trato gastrointestinal das larvas quando a inclusão dos probióticos foi feita a partir da dieta inerte, independentemente do tipo do probiótico, porém não houve efeito quando o procedimento foi realizado na água de cultivo. O uso de bactérias ácido lácticas não teve impacto significativo (p>0,05) sobre a sobrevivência, comprimento, peso fresco, IEL e ICL, apresentando resultados similares ao grupo controle. No entanto, o método de inclusão na dieta promoveu a colonização bacteriana no intestino, mas a inclusão diretamente na água também pode contribuir com a proteção externa dos animais e esse efeito é importante para avaliar a resistência contra patógenos em estudos futuros.

Referências

Adel, M., El-Sayed, A. F. M., Yeganeh, S., Dadar, M., & Giri, S. S. (2017). Effect of Potential Probiotic Lactococcus lactis Subsp. lactis on Growth Performance, Intestinal Microbiota, Digestive Enzyme Activities, and Disease Resistance of Litopenaeus vannamei. Probiotics and Antimicrobial Proteins, 9(2):150-156. https://doi.org/10.1007/s12602-016-9235-9.

Ambas, I., Suriawan, A., & Fotedar, R. (2013). Immunological responses of customised probiotics-fed marron, Cherax tenuimanus, (Smith 1912) when challenged with Vibrio mimicus. Fish and Shellfish Immunology, 35(2): 262-270. https://doi.org/10.1016/j.fsi.2013.04.026.

Anger, K. (2013). Neotropical Macrobrachium (caridea: palaemonidae): on the biology, origin, and radiation of freshwater-invading shrimp. Journal of Crustacean Biology, 33(2): 151-183. https://doi.org/10.1163/1937240X-00002124.

Araújo, M. C., & Valenti, W. C. (2007). Feeding habit of the Amazon river prawn Macrobrachium amazonicum larvae. Aquaculture, 265(1-4): 187-193. https://doi.org/10.1016/j.aquaculture.2007.01.016.

Bernal, M. G., Marrero, R. M., Campa-Córdova, A. I., & Mazon-Suástegui, J. M. (2017). Probiotic effect of Streptomyces strains alone or in combination with Bacillus and Lactobacillus in juveniles of the white shrimp Litopenaeus vannamei. Aquaculture International, 25(2): 927-939. https://doi.org/10.1007/s10499-016-0085-y.

Bidham, C. D., Meena, D. K., Behera, B. K., Pronob, D., Mohapatra, P. K. D., & Sharma, A. P. (2014). Probiotics in fish and shellfish culture: immunomodulatory and ecophysiological responses. Fish Physiology and Biochemistry, Springer. https://doi.org/10.1007/s10695-013-9897-0.

Chai, P. C., Song, X. L., Chen, G. F., Xu, H., & Huang, J. (2016). Dietary supplementation of probiotic Bacillus PC465 isolated from the gut of Fenneropenaeus chinensis improves the health status and resistance of Litopenaeus vannamei against white spot syndrome virus. Fish and Shellfish Immunology, 54, 602-611. https://doi.org/10.1016/j.fsi.2016.05.011.

Chong-Carrillo, O., Vega-Villasante, F., Arencibia-Jorge, R., Akintola, S. L., Michán-Aguirre, L., & Cupul-Magaña, F. G. (2015). Research on the river shrimps of the genus Macrobrachium (Bate, 1868) (Decapoda: Caridea: Palaemonidae) with known or potential economic importance : strengths and weaknesses shown through scientometrics. Latin american journal of aquatic research, 43(4), 684–690 https://doi.org/10.3856/vol43-issue4-fulltext-7.

Daniels, C. L., Merrifield, D. L., Ringø, E., & Davies, S. J. (2013). Probiotic, prebiotic and synbiotic applications for the improvement of larval European lobster (Homarus gammarus) culture. Aquaculture, 416 (December), 396-406. https://doi.org/10.1016/j.aquaculture.2013.08.001.

Dash, G., Raman, R. P., Pani Prasad, K., Makesh, M., Pradeep, M. A., & Sen, S. (2015). Evaluation of paraprobiotic applicability of Lactobacillus plantarum in improving the immune response and disease protection in giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879). Fish and Shellfish Immunology, 43(1), 167–174. https://doi.org/10.1016/j.fsi.2014.12.007.

Dash, G., Raman, R. P., Prasad, K. P., Marappan, M., Pradeep, M. A., & Sen, S. (2016). Evaluation of Lactobacillus plantarum as a water additive on host associated microflora, growth, feed efficiency and immune response of giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879). Aquaculture Research, 47(3), 804–818. https://doi.org/10.1111/are.12539.

Dash, G., Raman, R. P., Prasad, K. P., Marappan, M., Pradeep, M. A., & Sen, S. (2014). Evaluation of Lactobacillus plantarum as a water additive on host associated microflora, growth, feed efficiency and immune response of giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879). Aquaculture Research, 47(3), 804-818. https://doi.org/10.1111/are.12539.

Dawood, M. A. O., Koshio, S., Abdel-Daim, M. M., & Van Doan, H. (2018). Probiotic application for sustainable aquaculture. Reviews in Aquaculture, 11(3), 907-924. https://doi.org/10.1111/raq.12272.

Dhont, J., Wille, M., Frinsko, M., Coyle, S. D., & Sorgeloos, P. (2010). Larval Feeds and Feeding. In M. B. New, W. C. Valenti, J. H. Tidwell, L. R. D’Abramo, & M. N. Kutty (Eds.), Freshwater prawns: biology and farming (pp. 86–107). Wiley-Blackwell.

Doyle, J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedures for small amounts of fresh leaf tissue. Phytochemical Bulletin, 19(1), 11-15.

Estrela, C. (2018). Metodologia científica: ciência, ensino, pesquisa. (3rd ed.). Artes Médicas.

Fang, H., Wang, B., Jiang, K., Liu, M., & Wang, L. (2020). Effects of Lactobacillus pentosus HC-2 on the growth performance, intestinal morphology, immune-related genes and intestinal microbiota of Penaeus vannamei affected by aflatoxin B1. Aquaculture, 525 (March), 735289. https://doi.org/10.1016/j.aquaculture.2020.735289.

Flegel, T.W. (2019). A future vision for disease control in shrimp aquaculture. Journal of the World Aquaculture Society, 50(2), 249-266. https://doi.org/10.1111/jwas.12589.

Franco, R., Martín, L., Arenal, A., Santiesteban, D., Sotolongo, J., Cabrera, H., Mejías, J., Rodríguez, G., Moreno, A. G., Pimentel, E., & Castillo, N. M. (2016). Evaluation of two probiotics used during farm production of white shrimp Litopenaeus vannamei (Crustacea: Decapoda). Aquaculture Research, 48(4), 1936–1950. https://doi.org/10.1111/are.13031.

Giri, S. S., Sukumaran, V., & Oviya, M. (2013). Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita. Fish and Shellfish Immunology, 34(2), 660–666. https://doi.org/10.1016/j.fsi.2012.12.008.

Habib, A., Das, N. G., & Hossain, M. B. (2014). Growth Performance and Survival Rate of Macrobrachium rosenbergii (De Man, 1979) Larvae Using Different Doses of Probiotics. Pakistan Journal of Biological Sciences, 17(7), 920-924. https://doi.org/10.3923/pjbs.2014.920.924.

Hooper, L.V. (2015). Epithelial cell contributions to intestinal immunity. In: Advances in immunology, 126, 129-172. https://doi.org/10.1016/bs.ai.2014.11.003.

Jamali, H., Imani, A., Abdollahi, D., Roozbehfar, R., & Isari, A. (2015). Use of Probiotic Bacillus spp. in Rotifer (Brachionus plicatilis) and Artemia (Artemia urmiana) Enrichment: Effects on Growth and Survival of Pacific White Shrimp Litopenaeus vannamei, Larvae. Probiotics and Antimicrobial Proteins, 7(2), 118-125. https://doi.org/10.1007/s12602-015-9189-3.

Keysami, M. A., Saad, C. R., Sijam, K., Daud, H. M., & Alimon, A. R. (2007). Effect of Bacillus subtilis on growth development and survival of larvae Macrobrachium rosenbergii (de Man). Aquaculture Nutrition, 13(2), 131-136. https://doi.org/10.1111/j.1365-2095.2007.00463.x.

Kumar, V., Roy, S., Maena, D. K., & Sarkar, U. K. (2016). Application of Probiotics in Shrimp Aquaculture: Importance, Mechanisms of Action, and Methods of Administration. Reviews in Fisheries Science and Aquaculture, 24(4), 342-368. https://doi.org/10.1080/23308249.2016.1193841.

Lima, J. F., Silva, L. M. A., Silva, T. C., Garcia, J. S., Pereira, I. S., & Amaral, K. D. S. (2014). Reproductive aspects of Macrobrachium amazonicum (Decapoda: Palaemonidae) in the State of Amapa, Amazon River mouth. Acta Amazonica, 44(2), 245-254. https://doi.org/10.1590/S0044-59672014000200010.

Luis-Villaseñor, I. E., Macías-Rodríguez, M. E., Gómez-Gil, B., Ascencio-Valle, F., & Campa-Córdova, A. I. (2011). Beneficial effects of four Bacillus strains on the larval cultivation of Litopenaeus vannamei. Aquaculture, 321(1-2), 136-144. https://doi.org/10.1016/j.aquaculture.2011.08.036.

Maciel, C. R., & Valenti, W. C. (2009). Biology, Fisheries, and Aquaculture of the Amazon River Prawn Macrobrachium amazonicum: A Review. Nauplius, 17(2), 61-79.

Maciel, C. R., & Valenti, W. C. (2014). Assessing the potential of partil replacing of artemia by practical inert diet in the larviculture of the Amazon river prawn. Boletim do Instituto de Pesca, 40(1), 69-78. https://www.pesca.sp.gov.br/boletim/index.php/bip/article/view/1022,

Maciel, C. R., New, M. B., & Valenti, W. C. (2012). The Predation of Artemia Nauplii by the Larvae of the Amazon River Prawn, Macrobrachium amazonicum (Heller, 1862), is Affected by Prey Density, Time of Day, and Ontogenetic Development. Journal of World Aquaculture Society, 43(5), 659-669. https://doi.org/10.1111/j.1749-7345.2012.00599.x.

Manzi, J. J., Maddox, M. B., & Sandifer, P. A. (1977). Algal supplement enhancement of Macrobrachium rosenbergii (De Man) larviculture. Proceedings of the World Mariculture Society, 8(1-4), 207-223. https://doi.org/10.1111/j.1749-7345.1977.tb00119.x.

Marques, M. H. C., Silva, I, C., Zacardi, D. M., Santos, M. A. S., Brabo, M. F., & Maciel, C. R. (2020). Perfil do consumidor de camarão-da-Amazônia no Estado do Pará: socioeconômica, frequência de consumo e preferências. Research, Society and Development, 9(9), e525997316. https://doi.org/10.33448/rsd-v9i9.7316.

Moraes-Valenti, P., & Valenti, W. C. (2010). Culture of the Amazon river prawn Macrobrachium amazonicum. In M. B. New, W. C. Valenti, J. H. Tidwell, L. R. D’Abramo, & M. N. Kutty (Eds.), Freshwater prawns: biology and farming (pp. 485-501). Wiley-Blackwell.

Nimrat, S., Suksawat, S., Boonthai, T., & Vuthiphandchai, V. (2012). Potential Bacillus probiotics enhance bacterial numbers, water quality and growth during early development of white shrimp (Litopenaeus vannamei). Veterinary Microbiology, 159(3-4), 443-450. https://doi.org/10.1016/j.vetmic.2012.04.029.

Olmos, J., Acosta, M., Mendoza, G., & Pitones, V. (2020). Bacillus subtilis, an ideal probiotic bacterium to shrimp and fish aquaculture that increase feed digestibility, prevent microbial diseases, and avoid water pollution. Archives of Microbiology, 202(3), 427-435. https://doi.org/10.1007/s00203-019-01757-2.

Pereira, A. S., Shitsuka, D. M., Perreira, F. J., & Shitsuka, R. (2018). Metodologia da Pesquisa Científica (1st ed.). UFSM.

Queiroz, L. D., Abrunhosa, F. A., & Maciel, C. R. (2011). Ontogenesis and functional morphology of the digestive system of the freshwater prawn, Macrobrachium amazonicum (Decapoda: Palaemonidae). Zoologia, 28(3), 395–402. https://doi.org/10.1590/S1984-46702011000300014.

Ramadhani, D. E., Widanarni, D., & Sukenda, S. (2019). Microencapsulation of probiotics and its applications with prebiotic in Pacific white shrimp larvae through Artemia sp. Jurnal Akuakultur Indonesia, 18(2), 130-140. https://doi.org/10.19027/jai.18.2.130-140.

Ringø, E., Doan, H. Van, Lee, S., & Song, S. K. (2019). Lactic Acid Bacteria in Shellfish: Possibilities and Challenges. Reviews in Fisheries Science and Aquaculture, 28(2), 139–169. https://doi.org/10.1080/23308249.2019.1683151.

Seenivasan, C., Radhakrishnan, S., Shanthi, R., Muralisankar, T., & Saravana Bhavan, P. (2014). Effect of Lactobacillus sporogenes on survival, growth, biochemical constituents and energy utilization of freshwater prawn Macrobrachium rosenbergii post larvae. The Journal of Basic & Applied Zoology, 67(2), 19–24. https://doi.org/10.1016/j.jobaz.2013.12.002.

Silveira, D. S., Silva, A. S., Dias, J. A. R., Souza, N. C., Fujimoto, R.Y., & Cordeiro, C. A. M. (2015). Isolamento e estudos frente a patógenos in vitro de bactérias ácido láticas do Macrobrachium amazonicum (Heler, 1862) com potencial probiótico. Resumo do Congresso Brasileiro de Engenharia de Pesca. São Luiz.

Talib, A., Onn, K. K., Chowdury, M. A., Din, W. M. W., & Yahya, K. (2017). The beneficial effects of multispecies Bacillus as probiotics in enhancing culture performance for mud crab Scylla paramamosain larval culture. Aquaculture International, 25(2), 849-866. https://doi.org/10.1007/s10499-016-0070-5.

Talpur, A. D., Ikhwanuddin, M., Abdullah, M. D. D., & Bolong, A. A. (2013). Indigenous Lactobacillus plantarum as probiotic for larviculture of blue swimming crab, Portunus pelagicus (Linnaeus, 1758): effects on survival, digestive enzyme activities and water quality. Aquaculture, 416-417, 173-178. https://doi.org/10.1016/j.aquaculture.2013.09.018.

Tayamen, M., & Brown, J. H. (1999). A condition index for evaluating larval quality of Macrobrachium rosenbergii (De Man, 1879). Aquaculture Research, 30(11-12), 917-922. https://doi.org/10.1046/j.1365-2109.1999.00411.x.

Toledo, A., Castillo, M. N., Carrillo, O., & Arenal, A. (2018). Probiotics, a Reality in Shrimp Culture Review Article. Revista de Producción Animal, 30, 59-73. https://doi.org/10.1016/j.aquaculture.2018.10.018.

Tuan, T. N., Duc, P. M., & Hatai, K. (2013). Overview of the use of probiotics in aquaculture. International Journal of Research in Fisheries and Aquaculture, 3(3), 89–97.

Valenti, W. C., Mallasen, M., & Silva, C. A. (1998). Larvicultura em sistema fechado dinâmico. In W. C. Valenti (Ed.), Carcinicultura de Água Doce: tecnologias para a produção de camarões (pp. 112–139). Fundacão de Amparo à Pesquisa do Estado de São Paulo (FAPESP), São Paulo e Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA).

Verchuere, L., Rombaut, G., Sorgeloos, P., & Verstraete, W. (2000). Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews, 64(4), 655-671. https://doi.org/10.1128/mmbr.64.4.655-671.2000.

Vieira, F. N., Jatobá, A., Mouriño, J. L. P.,Vieira, E. A., Soares, M., da Silva, B. C., Seiffert, W. Q., Martins, M. L., & Vinatea, L. A. (2013). In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture. Pesquisa Agropecuaria Brasileira, 48(8), 998-1004. https://doi.org/10.1590/S0100-204X2013000800027.

Xue, M., Wen, C., Liang, H., Ding, M., Wu, Y., & Li, X. (2016). In vivo evaluation of the effects of commercial Bacillus probiotics on survival and development of Litopenaeus vannamei larvae during the early hatchery period. Aquaculture Research, 47(5), 1661-1669. https://doi.org/10.1111/are.12719.

Ziaei-Nejad, S., Rezaei, M. H., Takami, G. A., Lovett, D. L., Mirvaghefi, A. R., & Shakouri, M. (2006). The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture, 252(2-4), 516-524. https://doi.org/10.1016/j.aquaculture.2005.07.021.

Zokaeifar, H., Babaei, N., Saad, C. R., Kamarudin, M. S., Sijam, K., & Balcazar, J. L. (2014). Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei. Fish and Shellfish Immunology, 36(1), 68–74. https://doi.org/10.1016/j.fsi.2013.10.007.

Downloads

Publicado

10/11/2021

Como Citar

AVIZ, M. A. B. de; MACIEL, C. M. T. .; CORDEIRO, C. A. M. .; FUGIMOTO, R. Y.; MACIEL, C. R. Incorporação de bactérias probióticas no cultivo de larvas de Macrobrachium amazonicum (Heller, 1862). Research, Society and Development, [S. l.], v. 10, n. 14, p. e465101421721, 2021. DOI: 10.33448/rsd-v10i14.21721. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/21721. Acesso em: 3 set. 2024.

Edição

Seção

Ciências Agrárias e Biológicas