Potencial aplicação agroalimentar do extrato de resíduos de seriguela (Spondias purpurea L.) e nanoZnO como agentes antimicrobianos, antipatogênicos e antivirulência
DOI:
https://doi.org/10.33448/rsd-v11i1.25033Palavras-chave:
Bioativos de fonte vegetal; Nanopartículas; Atividade antimicrobiana; Antipatogenicidade; Antivirulência; Sistema de secreção tipo três; Quorum sensing.Resumo
O impacto de microrganismos fitopatogênicos em diversas safras causa perdas significativas para indústria agroalimentícia, deterioração na cadeia alimentar e de armazenamento. Nanopartículas e extratos vegetais têm se destacado por suas propriedades antimicrobianas applicadas em embalagens, agricultura, sistemas de distribuição de medicamentos e outras abordagens médicas. Nos últimos anos, este grupo tem estudado a aplicação de nanopartículas de ZnO e resíduos agroindustriais em revestimentos/filmes comestíveis para alimentos. Este trabalho teve como objetivo avaliar características ativas do extrato de resíduos do processamento da seriguela e do nanoZnO quanto à atividade inibitória contra sistemas de patogenicidade e virulência bacteriana: TTSS (Sistema de Secreção Tipo Três) e QS (Quorum Sensing) para Pseudomonas savastanoi. Além da ação antibacteriana (área de inibição) contra espécies de Curtobacterium, Clavibacter, E. coli, Xanthomonas e Serratia, e da antifúngica contra Botrytis cinerea (redução do tamanho da colônia). Extrato a 60% inibiu a ativação do sistema QS e TTSS em 20,26% e 13,54%, respectivamente; enquanto nanoZnO a 3% reduziu 46,77% QS e aumentou 302,88% TTSS. Extrato sem diluição inibiu o crescimento de Clavibacter michiganensis pv michiganensis (Gram-positiva) e Xanthomonas phaseoli (Gram-negativa), zona inibitória 94,25 mm2 e 452,39 mm2 respectivamente. Esta última também inibida por nanoZnO 1 e 2% (138,23 mm2) e 3% (275,67 mm2). Extrato puro inibiu em 17,38% o crescimento da colônia fúngica e o nanoZnO (1 e 3%) em 33,08%. Por fim, os agentes ativos estudados mostraram-se promissores na prevenção de doenças fitopatogênicas consequentemente perdas econômicas, filmes/revestimentos de alimentos e o extrato como biopesticida reduzindo o impacto ambiental.
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