Produção sustentável de biossurfactante por Issatchenkia orientalis UCP 1603 usando substratos renováveis

Autores

DOI:

https://doi.org/10.33448/rsd-v11i4.27174

Palavras-chave:

Tensioativo microbiano; Subprodutos agroindustriais; Issatchenkia orientalis.

Resumo

Os surfactantes biológicos são moléculas anfipáticas produzidas por micro-organismos e são considerados compostos multifuncionais do século XXI. O presente trabalho teve como objetivo utilizar substratos renováveis de baixo custo para a produção econômica de biossurfactante por Issatchenkia orientalis UCP 1603. As fermentações foram realizadas a 28°C e 150 rpm por 72 h, utilizando subprodutos agroindustriais (manipueira, milhocina e óleo de soja pós-fritura) como substratos, de acordo com um planejamento fatorial completo de 23 para identificar sua influência na produção de biossurfactante. Os resultados mostraram a capacidade da levedura em produzir biossurfactante em todas as condições do planejamento, destacando-se a condição 4 devido à maior redução da tensão superficial (de 72 para 29,7 mN/m). As análises estatísticas evidenciaram a influência significativa da manipueira e milhocina na produção de biossurfactante. As propriedades tensoativas da biomolécula foram confirmadas por teste de parafilme e índice de emulsificação. Este estudo evidenciou I. orientalis como uma levedura promissora produtora de biossurfactante, com excelente capacidade e elevado potencial biotecnológico de transformação de subprodutos agroindustriais.

Biografia do Autor

Tainã Crisia de Souza Fonseca , Federal Rural University of Pernambuco

Rede  de Pós-graduação em Biotecnologia   RENORBIO

Adriana Ferreira de Souza, Catholic University of Pernambuco

Núcleo de Pesquisa  en Ciências Ambientais  e Biotecnologia-NPCIAMB

Patrícia Nunes dos Santos, Federal Rural University of Pernambuco

Rede de Pós-graduação Programa em Biotecnologia -RENORBIO

Paulo Henrique da Silva, Federal Rural University of Pernambuco

Rede de Pós-graduação Programa de Biotecnologia RENORBIO

Dayana Montero Rodriguez, Catholic University of Pernambuco

Nucleo de Pesquisa en Ciências Ambientais e Biotecnologia-NPCIAMB

Luiz Oliveira Costa, Catholic University of Pernambuco

Coordenador do Curso de graduaçãoem Ciências Biológicas

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Publicado

14/03/2022

Como Citar

FONSECA , T. C. de S. .; SOUZA, A. F. de .; SANTOS, P. N. dos .; SILVA, P. H. da .; RODRIGUEZ, D. M. .; COSTA, L. O. .; CAMPOS-TAKAKI, G. M. Produção sustentável de biossurfactante por Issatchenkia orientalis UCP 1603 usando substratos renováveis. Research, Society and Development, [S. l.], v. 11, n. 4, p. e16111427174, 2022. DOI: 10.33448/rsd-v11i4.27174. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/27174. Acesso em: 17 jul. 2024.

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Engenharias