Antimicrobial peptides expressed in the fat body of Spodoptera frugiperda (Lepidoptera: Noctuidae) in response to the microbial challenge

Elisiane Pinto  Feistler; Alexandre Maller; Rita de Cássia Garcia  Simão; Marina Kimiko  Kadowaki; Luis Francisco Angeli  Alves; José Luis da Conceição  Silva

doi:10.33448/rsd-v11i13.35263

Autores

Elisiane Pinto Feistler State University of Western Paraná https://orcid.org/0000-0001-6012-525X
Alexandre Maller State University of Western Paraná https://orcid.org/0000-0002-8065-9184
Rita de Cássia Garcia Simão State University of Western Paraná https://orcid.org/0000-0003-2999-1218
Marina Kimiko Kadowaki State University of Western Paraná http://orcid.org/0000-0002-9174-5811
Luis Francisco Angeli Alves State University of Western Paraná https://orcid.org/0000-0002-3102-764X
José Luis da Conceição Silva State University of Western Paraná https://orcid.org/0000-0002-5460-8797

DOI:

https://doi.org/10.33448/rsd-v11i13.35263

Palavras-chave:

Peptídeos antimicrobianos; Beauveria bassiana; Corpo gordo; Sistema imunológico; Expressão genética.

Resumo

Spodoptera frugiperda (Lepidoptera: Noctuidae) é a principal praga da cultura do milho e tem grande impacto na agricultura mundial. Vários estudos sobre o controle biológico desta espécie têm sido realizados, e estes demonstram resistência ao controle biológico e agrotóxicos. No entanto, o sistema imune humoral de S. frugiperda ainda não é completamente conhecido em relação à resposta imune inata para agentes de controle biológico. O objetivo deste estudo foi avaliar a expressão dos genes Gloverin, Sf-galerimycin e Attacin no corpo gorduroso de larvas no 6º instar de desenvolvimento. Nosso trabalho confirmou a expressão de Sf-galerimicina em resposta ao desafio bacteriano, 24 h após a inoculação, e revelou pela primeira vez a expressão de Gloverina e Attacina, tanto com o desafio bacteriano quanto com o fungo entomopatogênico Beauveria bassiana. Os genes Gloverina e Attacina foram regulados positivamente nas condições analisadas. Além disso, revelamos a presença de dois prováveis peptídeos antimicrobianos na hemolinfa, induzidos 24 h após o desafio com os microrganismos. A banda b de 4,7 kDa é provavelmente um peptídeo do tipo defensina, e a banda a de 6,1 kDa é um peptídeo ainda não relatado em S. frugiperda.

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Peptídeos antimicrobianos expressos no corpo gorduroso de Spodoptera frugiperda (Lepidoptera: Noctuidae) em resposta ao desafio microbiano

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