Efeito protetor do extrato amazônico de Himatanthus sucuuba em Drosophila melanogaster expostas ao Paraquat
DOI:
https://doi.org/10.33448/rsd-v11i17.38931Palavras-chave:
Drosophila melanogaster; Neuroproteção; Antioxidantes; Plantas amazônicas.Resumo
A floresta amazônica é uma importante fonte de conhecimento científico, por isso, diversos grupos de pesquisa buscam compreender a atuação de compostos amazônicos nas doenças. Dentre a vasta flora, Himatanthus sucuuba apresenta uma variedade de fins terapêuticos, sendo aqui, utilizada pela primeira vez na neuroproteção induzida pelo Paraquat (PQ) em Drosophila melanogaster. Em nosso estudo, realizamos ensaios fitoquímicos com o extrato hidro alcoólico de H. sucuuba revelando qualitativamente classes de metabólitos secundários e quantitativamente fenóis totais (43,33 mg EAG/g extrato-1), flavonoides totais (44,09 mg EAG/G Extrato-1) e atividade antioxidante via DPPH e ABTS. Ainda, a exposição D. melanogaster adultas (linhagem selvagem, Canton Special) ao PQ por 15 dias causou elevado estresse oxidativo, como evidenciado pelos elevados níveis de proteínas carboniladas, lactato e atividades de acetilcolinesterase e citrato sintase. Entretanto, a dieta suplementada com H. sucuuba (0.1 mg/mL) por 15 dias impediu os danos do estresse oxidativo desencadeados pelo PQ. O nosso estudo visa demonstrar o efeito protetor do extrato de H. sucuuba em D. melanogaster exposta ao PQ. Baseados em nossos resultados, sugerimos que os extratos provenientes das cascas de H. sucuuba podem prevenir ou minimizar doenças humanas causadas pelo estresse oxidativo, para tanto, demais estudos relacionados aos mecanismos envolvidos em tal ação serão necessários.
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