Determinação do potencial fenólico, antioxidante e antimicrobiano de extratos foliares de Pereskia grandifolia Haw
DOI:
https://doi.org/10.33448/rsd-v9i10.8483Palavras-chave:
PANC; Ora-pro-nóbis; Compostos bioativos; Espectrofotometria; Patógenos.Resumo
Research on Unconventional Food Plants (UFP) for the presence of bioactive and antimicrobial compounds is scarce and the expansion of knowledge regarding their properties becomes necessary. In this study, four different types of extracts (aqueous and hydroalcoholic obtained by reflux and sonication) from ora-pro-nóbis (Pereskia grandifolia Haw) were investigated for the presence of phenolic compounds, antioxidants, and antibacterials. The extracts of Pereksia grandifolia Haw obtained by reflux were more efficient than sonication because they extracted a greater amount of phenolic compounds, antioxidants, flavones, and flavonols. In relation to the solvent, it was detect that the hydroalcoholic allowed a greater quantification of total flavonoids (flavones and flavonols, flavanones and di-hydroflavonols) and antioxidants (measured by the methods of DPPH, molybdate and chelating power). When water was used as a solvent extractor, larger amounts of total phenolic compounds and antioxidants (measured by DPPH and chelating power) were observed. All extracts presented inhibitory activity against Staphylococcus aureus and Pseudomonas aeruginosa, and aqueous extracts obtained by reflux and sonication being the largest inhibition halos. However, when the minimum bactericidal concentration was determined, only the hydroalcoholic extract obtained by reflux showed an effective result at the tested concentrations. The characterization and quantification of the phenolic, antioxidant and antibacterial compounds of P. grandifolia Haw provide important information to increase the use of this plant, as an alternative source of compounds beneficial to health, and with potential antimicrobial activity.
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