Microbiological analysis of training environments and their correlation with bacterial resistance – Teresina, Piauí 2025
DOI:
https://doi.org/10.33448/rsd-v14i11.49981Keywords:
Microbial contanination, Bacterial resistance, Gyms, Staphylococci, Surface hygiene.Abstract
This study aimed to analyze the presence, growth, and bacterial resistance profile of microorganisms isolated from gym equipment surfaces in Teresina, Brazil. An experimental field study was conducted in three fitness centers, with samples collected from treadmills, leg extension machines, and straight bars. The samples were cultured on selective media and identified through Gram staining and biochemical tests (catalase and coagulase). Significant bacterial growth was observed in all samples, with a predominance of Gram-positive cocci, mainly staphylococci, indicating a microbiota typical of human contact. The antibiogram revealed that all isolates were sensitive to the tested antibiotics, showing no bacterial resistance. It is concluded that gyms can act as reservoirs for potentially pathogenic microorganisms, highlighting the importance of effective cleaning protocols and user awareness to prevent cross-contamination and ensure safer environments for physical activity.
References
Agência Nacional de Vigilância Sanitária (ANVISA). (2020). Nota Técnica nº 04/2020 – Orientações para serviços de saúde: medidas de prevenção e controle que devem ser adotadas durante a assistência aos casos suspeitos ou confirmados de infecção pelo novo coronavírus (SARS-CoV-2). Brasília: ANVISA. Disponível em: https://www.gov.br/anvisa
Ananias, B. C. S. (2024). Avaliação da limpeza e desinfecção de academias para prevenção de infecções: comparando ambientes públicos e privados [Trabalho de Conclusão de Curso, Universidade Federal de Juiz de Fora].
Bilung, L. M., Tahar, A. S., Kira, R., Rozali, A. A. M., & Apun, K. (2018). High Occurrence of Staphylococcus aureus Isolated from Fitness Equipment from Selected Gymnasiums. Journal of Environmental and Public Health, 2018, 4592830. https://doi.org/10.1155/2018/4592830
Browne, F. A., et al. (2023). Effectiveness of surface disinfection methods in preventing cross-contamination in shared environments. Journal of Hospital Infection, 135, 112–120. Disponível em: https://doi.org/10.1016/j.jhin.2023.03.008
Browne, K., White, N., Tehan, P., Russo, P. L., Amin, M., Stewardson, A. J., Cheng, A. C., Graham, K., O’Kane, G., King, J., Kiernan, M., Brain, D., & Mitchell, B. G. (2023). A randomised controlled trial investigating the effect of improving the cleaning and disinfection of shared medical equipment on healthcare-associated infections: The CLEaning and Enhanced disiNfection (CLEEN) study. Trials, 24(1), 133. Disponível em: https://doi.org/10.1186/s13063-023-07144-z
Cooper, E. E., et al. (2021). Microbial contamination and hygiene practices in fitness centers: Implications for community health. International Journal of Environmental Research and Public Health, 18(7), 3520. Disponível em: https://doi.org/10.3390/ijerph18073520
Fatemeh, Z. S., et al. (2021). Academias: Avaliação dos tipos de bioaerossóis bacterianos e concentrações no ar interno de academias. PubMed.Disponível em: https://pubmed.ncbi.nlm.nih.gov/33400007/
Frota, O. P., et al. (2020). Medidas de prevenção e controle de infecção relacionadas à assistência à saúde: manual técnico. Brasília: Agência Nacional de Vigilância Sanitária – ANVISA. Disponível em: https://www.gov.br/anvisa
Godoi, B. K., et al. (2023). Resistência antimicrobiana: prevalência, distribuição geográfica e diversidade genômica de populações de Staphylococcus aureus resistentes à meticilina (MRSA) estudadas no Brasil (2012–2022) [Trabalho acadêmico, Universidade Federal de Santa Catarina]. Repositório Institucional UFSC. Disponível em: https://repositorio.ufsc.br/handle/123456789/248733
Kiborus, L. J., et al. (2025). Staphylococcus aureus: Caracterização da prevalência e resistência a antibióticos de espécies de Staphylococcus em uma academia no centro de Kentucky, EUA. MDPI. Disponível em: https://www.mdpi.com/2673947X/5/1/2
Li, X., et al. (2021). Assessment of hygiene practices and risk of microbial transmission in fitness centers: A cross-sectional study. BMC Public Health, 21(1), 1125. Disponível em: https://doi.org/10.1186/s12889-021-11125-4
Mengge, Z., et al. (2023). Equipamento de ginástica: Superfícies de equipamentos de ginástica como reservatórios de patógenos microbianos com potencial para transmissão de infecção bacteriana e resistência antimicrobiana. PubMed. Disponìvel em: https://pubmed.ncbi.nlm.nih.gov/37152727/
Noel, M. L., et al. (2014). Diversity of bacterial communities of fitness center surfaces in a U.S. metropolitan area. International Journal of Environmental Research and Public Health, 11(12), 12544–12561. Disponível em: https://doi.org/10.3390/ijerph111212544
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica [E-book gratuito]. Santa Maria, RS: Editora da UFSM.
Silva, E. N., dos Santos, T. C. F., Costa, B. E., Corrêa, M. S. L., Ferracioli Alvarenga, G. H., Aguiar, É. F., Natel, A., Cavicchioli, V. Q., dos Santos Silva, D. B., & de Mello Silva, N. (2021). Incidência de Staphylococcus aureus em equipamentos de uma academia de atividades físicas em Alfenas – MG. Research, Society and Development, 10(12), e15101220056. Disponível em: https://doi.org/10.33448/rsd-v10i12.20056
Silva, L. R., et al. (2021). Avaliação da adesão às práticas de higienização de equipamentos em academias de ginástica. Revista Brasileira de Ciências do Esporte, 43(2), 1–10. Disponível em: https://doi.org/10.1590/rbce.2021.v43.121
Shitsuka, R., Shitsuka, D. M., Parreira, F. J., & Pereira, A. S. (2014). Matemática fundamental para a tecnologia (2ª ed.). São Paulo, SP: Editora Érica.
Szulc, A. L., et al. (2023). Microbiological contamination of fitness equipment and the effectiveness of disinfection procedures in gyms. International Journal of Environmental Research and Public Health, 20(4), 2895. Disponível em: https://doi.org/10.3390/ijerph20042895
Szulc, J., Okrasa, M., Ryngajłło, M., Pielech-Przybylska, K., & Gutarowska, B. (2023). Markers of chemical and microbiological contamination of the air in sport centers. Molecules, 28(8), 3560. Disponível em: https://doi.org/10.3390/molecules28083560
Turkstani, M. A., Sultan, R. M. S., Al-Hindi, R. R., & Ahmed, M. M. M. (2021). Molecular identification of microbial contaminations in the fitness center in Makkah region. Bioscience Journal, 37, e37020. Disponível em: https://doi.org/10.14393/BJ-v37n0a2021-50400
Weissfeld, A. S. (2015). Infecções na academia: Superfícies de academia na prevalência de infecções. ScienceDirect. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0196439915000422
Dalman, M., Bhatta, S., Nagajothi, N., Thapaliya, D., Olson, H., Naimi, H. M., & Smith, T. C. (2019). Characterizing the molecular epidemiology of Staphylococcus aureus across and within fitness facility types. BMC Infectious Diseases, 19, 69. Disponível em: https://doi.org/10.1186/s12879-019-3699-7
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Copyright (c) 2025 Júlia Guerra Fernandes Almeida, Camila Andrade de Azevedo, Glória Maria da Silva Alencar, Laiz Marcielle Silva Almeida Lobo, Michely Laiany Vieira Moura

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