Vigor tests for basil seeds

Authors

DOI:

https://doi.org/10.33448/rsd-v14i11.50259

Keywords:

Ocimum basilicum L., Physiological quality, Germination, Electrical conductivity, Accelerated aging.

Abstract

Basil (Ocimum basilicum L.) is an aromatic and medicinal species of great economic importance, widely used in the culinary, pharmaceutical, and cosmetic sectors. The physiological quality of seeds is crucial for seedling establishment and for the productive success of the crop, with vigor tests serving as essential tools for selecting seed lots with superior performance. Therefore, this study aimed to identify the most efficient vigor tests for assessing the physiological potential of basil seeds. The experiment followed a completely randomized design with four replications, using five seed lots of the cultivar Basilicão, supplied by Isla Sementes®. The lots were evaluated through germination tests (first count and final percentage), accelerated aging (traditional and with saline solution), electrical conductivity, seedling length, and seedling emergence at seven and fourteen days after sowing. The results revealed significant differences among seed lots in most tests, except for electrical conductivity and first germination count. The outcomes of the vigor tests were consistent with the seedling emergence results, with seedling length and both traditional and saline-solution accelerated aging tests showing greater accuracy in differentiating seed lots in terms of vigor. It is concluded that the traditional and saline-solution accelerated aging tests, along with seedling length evaluation, are effective for assessing the vigor of basil seed lots.

References

AOSA. (1983). Seed vigor testing handbook (Contribution No. 32). Association of Official Seed Analysts.

AOSA. (2009). Seed vigor testing handbook (Contribution No. 32). Association of Official Seed Analysts.

Barbosa, R. M., Costa, D. S., Carvalho, R. V., & Vieira, R. D. (2011). Teste de envelhecimento acelerado em sementes de plantas aromáticas. Revista Brasileira de Sementes, 33(4), 652–660.

Barbosa, R. M., Vieira, R. D., & Krzyzanowski, F. C. (2011). Envelhecimento acelerado em sementes: metodologias e aplicações. Revista Brasileira de Sementes, 33(1), 13–20.

Basu, A. C., Fernandes, R. A., Lima, T. S., & Moura, F. A. (2023). Seed vigor and post-harvest behavior in aromatic and medicinal species. Seed Science and Technology, 51(1), 101–112.

Brasil. (2009). Regras para análise de sementes. Ministério da Agricultura, Pecuária e Abastecimento.

Carvalho, F. A., Mendes, C. L., & Rocha, P. C. (2021). Influência de fatores ambientais na produção de óleo essencial de manjericão (Ocimum basilicum L.). Revista Brasileira de Plantas Medicinais, 23(3), 145–154.

Carvalho, N. M., & Nakagawa, J. (2012). Sementes: ciência, tecnologia e produção (5ª ed.). FUNEP.

Costa Neto, P. L. O. & Bekman, O. R. (2009). Análise Estatística da Decisão. Editora Blucher.

Delouche, J. C., & Baskin, C. C. (2018). Seed quality and storage behavior of cultivated aromatic plants. Seed Science Research, 28(3), 198–207.

Ferreira, D. F. (2019). SISVAR: A computer analysis system to fixed effects split plot type designs. Revista Brasileira de Biometria, 37(4), 529–535.

Ferreira, R. L., Silva, P. R., & Andrade, A. C. S. (2017). Fisiologia e tecnologia de sementes de plantas aromáticas. Revista Brasileira de Plantas Medicinais, 19(3), 512–520.

Fracaro, L. H. (2023). Germinação e vigor de sementes de alface tratadas com diferentes doses de microrganismos eficientes (Trabalho de conclusão de curso, Universidade Federal da Fronteira Sul). Universidade Federal da Fronteira Sul Repositório Institucional. https://rd.uffs.edu.br/handle/prefix/7243.

Jianhua, Z., & McDonald, M. B. (1996). The saturated salt accelerated aging test for small-seeded crops. Seed Science and Technology, 24(3), 493–503.

Krzyzanowski, F. C., Vieira, R. D., & França-Neto, J. B. (2021). Vigor de sementes: conceitos e testes (4ª ed.). ABRATES.

Lopes, E. C., Barros, C. M., & Costa, M. A. (2019). Propagação e desenvolvimento inicial de manjericão sob diferentes substratos. Horticultura Brasileira, 37(4), 495–502.

Marcos-Filho, J. (2015). Fisiologia de sementes de plantas cultivadas (2ª ed.). Londrina, PR: ABRATES.

Marcos-Filho, J. (2015). Seed vigor testing: An overview of the past, present and future perspective. Scientia Agricola, 72(4), 363–374.

Martins, L. F., & Pereira, J. R. (2018). Aspectos agronômicos e usos medicinais do manjericão (Ocimum basilicum L.). Revista Ciência Agronômica, 49(2), 201–209.

Oliveira, C. E. S., Silva, J. R., & Ferreira, T. R. (2022). Fisiologia de sementes e desempenho inicial de espécies medicinais e aromáticas. Revista Brasileira de Plantas Medicinais, 24(2), 85–94.

Oliveira, D. S., Costa, R. A., & Faria, G. M. (2022). Avaliação de testes de vigor em sementes de hortaliças. Revista de Agricultura Neotropical, 9(1), 37–45.

Pereira, A. S. et al. (2018). Metodologia da pesquisa científica. [free ebook]. Santa Maria. Editora da UFSM.

Rodo, A. B., Vieira, R. D., & Panobianco, M. (2000). Electrical conductivity test in corn seeds with different vigor levels. Seed Science and Technology, 28(3), 669–681.

Shitsuka, R. et al. (2014). Matemática fundamental para a tecnologia. (2ed). Editora Érica.

Published

2025-11-29

Issue

Section

Agrarian and Biological Sciences

How to Cite

Vigor tests for basil seeds. Research, Society and Development, [S. l.], v. 14, n. 11, p. e244141150259, 2025. DOI: 10.33448/rsd-v14i11.50259. Disponível em: https://rsdjournal.org/rsd/article/view/50259. Acesso em: 5 dec. 2025.