Atividade de biomarcadores bioquimicos em gafanhotos Abracris flavolineata (De Geer, 1773) (Orthoptera: Acrididae: Ommatolampidinae)
DOI:
https://doi.org/10.33448/rsd-v10i11.19877Palavras-chave:
Glutathiona S-transferase, Estresse Oxidativo, Enzima, Catalase.Resumo
Os biomarcadores bioquímicos são comumente usados em programas de monitoramento ambiental por serem sensíveis à presença de determinados poluentes. Assim, a resposta desse biomarcadores pode ser utilizada como um indicador de qualidade ambiental. O presente estudo teve como objetivos determinar a atividade das enzimas catalase (CAT) e glutationa-S-transferase (GST) de gafanhotos Abracris flavolineata (De Geer, 1773), coletados em área duas áreas de remanescente florestal na Serra da Jiboia (BA) e comparar a atividade destas enzimas entre machos e fêmeas. As coletas dos espécimes foram realizadas em dois pontos situados na Serra da Jiboia (Bahia, Brasil), denominados ‘Baixa de Areia’ e ‘Baixa Grande’. Os insetos foram capturados por meio de busca ativa com auxílio de rede entomológica, no período matutino, e esforço de coleta com 2,5 h de duração. No total, foram coletados 160 indivíduos, sendo 80 exemplares provenientes de cada ponto amostral, com 50 machos e 30 fêmeas. Após a identificação procedeu-se incisão na região lateral do abdômen para retirada do intestino médio, que foi utilizado para extração das enzimas CAT e GST. Os resultados obtidos demonstraram que a atividade da CAT e GST não variou significativamente entre os pontos de coletas, entretanto, em relação ao sexo, a atividade das enzimas foi significativamente maior nos machos (p<0,005), em ambos os pontos amostrados. Este estudo é pioneiro na avaliação das respostas da atividade da CAT e GST em gafanhotos no Brasil.
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