Evaluation of staining of light-cured composite resin samples with different surfaces exposed to mouthwashes with and without alcohol. In vitro study

Authors

DOI:

https://doi.org/10.33448/rsd-v14i9.49453

Keywords:

Esthetics, Composite resins, Mouthwashes.

Abstract

The present study was proposed to characterize whether there is staining of composite resin samples with different surface treatments after mouthwashes with and without alcohol use for limited period.  One hundred light-curing composite resin specimens, color EB1, with surface characteristics of untreated on one side of the sample, and polished on the other, distributed into 10 groups (G): G1- coffee, G2- distilled water, G3- 0.12% chlorhexidine digluconate with alcohol, G4- 0.12% chlorhexidine digluconate without alcohol, G5- cetylperidine chloride with alcohol, G6- cetylperidine chloride without alcohol, G7- essential oils with alcohol, G8- essential oils without alcohol, G9- stabilized chlorine dioxide at 0.02%, G10- 0.06% chlorhexidine digluconate with alcohol. In each group, 10 samples remained immersed in the products for a time of use mimicking 1 year. Three blind calibrated examiners assigned scores to characterize their staining. The Kruskal-Wallis test with a 5% significance level, complemented by the Student-Newman-Keuls test, showed that staining occurred, influenced by mouthwash and surface polishing. G1, G3, and G7 presented higher staining scores (p<0.01) regardless of whether the surface was polished or not. Within the limits of this study, it can be stated that the dentist must be attentive to the indication of mouthwashes for his patients, since, in the presence of aesthetic light-curing composite resin restorations, all promoted color change, and essential oils and 0.12% chlorhexidine associated with alcohol were related to greater staining on both polished and unpolished surfaces.

References

Alshehri, A., Binalrimal, S., Alrumi, L., Alhabeeb, Y. Y., Murshid, L. E., Alhalabi, F., Alqahtani, A. A. & Mustafa, M. (2025). Comparison of color stability between single-shade and conventional composite resins following immersion in staining solutions of coffee, cola, and distilled water. Medical science monito: International Medical Journal of Experimental and Clinical Research, 31, e946784. https://doi.org/10.12659/MSM.946784

Aragão, G.S., Falcão, R.M., Durães, Í. & Bezerra RB. (2016). Influência dos enxaguatórios bucais na rugosidade superficial de uma resina composta. Journal of Dentistry & Public Health (inactive Archive Only), 7(4). https://doi.org/10.17267/2596-3368dentistry.v7i4.936

Araújo, D.B., Campos, E.J., Martins, G.B., Araújo, M.T.B., Assis, M.S., Santos, T.S. & Costa, Y.K.S. (2015). Estudo dos enxaguatórios bucais disponíveis nas drogarias, farmácias e supermercados na cidade do Salvador, BA. Journal of Dentistry & Public Health (inactive Archive Only), 6(1). https://doi.org/10.17267/2596-3368dentistry.v6i1.656

Avalos, R.A., Medina-Sánchez, R., Castro-Ramirez, L., Ladera-Castañeda, M., Cervantes-Ganoza, L., Martínez-Campos, R. et al. (2023). In vitro color stability evaluation of three polished and unpolished nanohybrid resin composites immersed in a 0.12% chlorhexidine-based mouthwash at different times. Polymers. 15: 1339. doi: 10.3390/polym15061339.

Batista, A.D., Souza, V.T.P., Braga, L.F., Santos, F.R.C., Martins, M.A.S., Rodrigues, L.S. et al. (2023). Avaliação da rugosidade superficial de uma resina composta unicromática. Revistaft. 27(121). doi: 10.5281/zenodo.7829306

Brookes, Z., Teoh, L., Cieplik, F. & Kumar, P. (2023). Mouthwash effects on the oral microbiome: Are They good, bad, or balanced? International Dental Journal.73: s74− s81. doi: 10.1016/j.identj.2023.08.010

Cengiz, S., Yüzbas, E., Lu, I., Cengiz, M.I., Velioglu, N. & Sevimli, G. (2015). Color stability and surface roughness of a laboratory-processed composite resin as a function of mouthrinse. Journal of Esthetic and Restorative Dentistry. 27(5): 314–321. doi: 10.1111/jerd.12167

Dias, L.M., Araújo, D.B. & Araujo, M.T.B. (2013). Análise dos enxaguatórios e dentifrícios comercializados na Cidade do Salvador, Bahia. Rev Ciênc Méd Biol. 12(3): 344-349. doi: 10.9771/cmbio.v12i3.9094

Festuccia, M.S.C.C., Garcia, L.F.R., Cruvinel, D.R. & Souza, F.C.P.P. (2012). Color stability, surface roughness and microhardness of composites submitted to mouthrinsing action. J Appl Oral Sci. 20(2): 200-5. doi: 10.1590/s1678-77572012000200013

Fim, M.D., Zanoni, P.A., Silva, D.N. & Castro, M.C.C. (2020). Efeito da clorexidina com sistema antidescolorante associada a café na estabilidade da cor de uma resina composta nanoparticulada. RSBO. 17(2):122-9. doi: https://doi.org/10.21726/rsbo.v17i2.36

Gadonski, A.P., Feiber, M., Almeida, L., Naufel, F.S. & Schmitt, V.L. (2018). Avaliação do efeito cromático em resinas compostas nanoparticuladas submetidas a solução café. Rev Odontol UNESP. 47(3): 137-142. doi: http://dx.doi.org/10.1590/1807-2577.04318

Gonçalves, E.M., Portela Filho, E.P., Aragão, P.R.C., Segundo, T.C.P. & Lima, D.L.F. (2010). Grau de conhecimento dos cirurgiões-dentistas na prescrição de colutórios e dentifrícios. R. Periodontia. 20(4): 51-55.

Hamdy, T.M., Abdelnabi, A., Othman, M.S., Bayoumi, R.E., & Abdelraouf, R.M. (2023). Effect of different mouthwashes on the surface microhardness and color stability of dental nanohybrid resin composite. Polymers. 15: 815. doi: 10.3390/polym15040815

Laky, M., Brandl, S., Arslan, M., Boryshchuk, D., Cześnikiewicz-Guzik, M., Rausch-Fan, X., Moritz, A. & Laky, B. (2025). Color stability of frequently used aesthetic restorative materials following in vitro exposure to chlorhexidine- and octenidine-based mouthrinses. BMC Oral Health. 25:450. doi: 10.1186/s12903-025-05811-y

Leite, R.B., Lins, R.D.A.U., Barbosa, D.N., Morais, E.F. & Ramalho, O.C.S. (2018). Efeito de diferentes colutórios sobre a microdureza de resinas compostas fotopolimerizáveis. Rev Odontol UNESP. 47(3): 125-130. doi: https://doi.org/10.1590/1807-2577.07117

Mallmann, A., Barros, G.S., Cavalcanti, N.A., Marchi, G.M., Jacques, L.B. & Mathias, P. (2009). Influência de colutórios bucais na dureza de resinas compostas. RFO. 14(1): 32-36.

Nahsan, F.P.S., Ueda, J.K., Silva, J.O., Schmitt, V.L., Naufel, F.S., Formighieri, L.A. & Baseggio, W. (2009). Estabilidade de cor de resina composta após imersão em café, água e solução de clorexidina. Revista Brasileira de Pesquisa em Saúde. 11(2): 13-17.

Pereira, A. S. et al. (2018). Metodologia da pesquisa científica. [free ebook]. Santa Maria. Editora da UFSM

Pereira, R. & Phad, S.G. (2017). Comparative evaluation of 0.2% chlorhexidine mouth rinse with and without an antidiscoloration system: a clinical study. Journal of Contemporary Dentistry. 7(1): 53-56. doi: 10.5005/jp-journals-10031-1185

Port, E.Z., Zimmer, R., Reston, E.G. & Arossi, G.A. (2024). Avaliação de manchamento de uma resina composta nanohíbrida após acabamento e polimento por diferentes discos de lixa. Rev Odontol Bras Central. 33(92): 1-14. doi: 10.36065/robrac.v33i92.1599

Sampaio, G.A.M., Peixoto, L.R., Neves, G.V. & Barbosa, D.N. (2021). Effect of mouthwashes on color stability of composite resins: a systematic review. J Prosthet Dent. 126(3): 386-392. doi: 10.1016/j.prosdent.2020.08.001

Shitsuka, R. et al. (2014). Matemática fundamental da tecnologia. (2.ed). Editora Érica.

Silva Neto, J.M.A., Silva, L.E.E., Souza, C.C.B., Pereira, N.E.C. & Mendonça, I.C.G. (2021). Utilização de resinas compostas em dentes anteriores. REAS. 13(2). doi: https://doi.org/10.25248/REAS.e6583.2021

Veras, B.M.L., Menezes, G.P.S., Gomes Filho, V.V. & Silva, C.H.V. (2015). Comportamento clínico de resinas compostas em dentes posteriores. Revisão sistematizada da literatura. Odontol. Clín.-Cient. 14(3): 689 – 694.

Vieira, S. (2021). Introdução à bioestatística. Editora GEN/Guanabara Koogan.

Published

2025-09-01

Issue

Section

Health Sciences

How to Cite

Evaluation of staining of light-cured composite resin samples with different surfaces exposed to mouthwashes with and without alcohol. In vitro study. Research, Society and Development, [S. l.], v. 14, n. 9, p. e0214949453, 2025. DOI: 10.33448/rsd-v14i9.49453. Disponível em: https://rsdjournal.org/rsd/article/view/49453. Acesso em: 5 dec. 2025.