Phytochemical screening of cashew leaf extract (Anacardium occidentale L.) and cajui (Anardium microcarpum D.) by means of maceration process with different solvents
DOI:
https://doi.org/10.33448/rsd-v15i4.49218Keywords:
Anacardium occidentale, Anacardium microcarpum, Phytochemistry, Secondary metabolites, Solvents.Abstract
This study aimed to perform a phytochemical screening of extracts from the leaves of Anacardium occidentale L. (cashew) and Anacardium microcarpum D. (cajui), based on the maceration of leaves using different solvents: distilled water, 70% ethanol, PA acetone, and PA hexane. The investigation sought to identify classes of secondary metabolites with therapeutic potential, such as alkaloids, flavonoids, tannins, and saponins, through specific qualitative tests. The use of solvents with varying polarities allowed for the evaluation of each solvent’s extraction efficiency in obtaining bioactive compounds. The results showed that the ethanolic extract presented the highest phytochemical diversity for both species, followed by aqueous and acetone extracts, while the hexane extract was the least efficient. Alkaloids were consistently present in all extracts, whereas flavonoids, tannins, and saponins varied depending on the solvent used. These findings reinforce the importance of solvent selection in phytochemical extraction and highlight the biotechnological and therapeutic potential of the studied species. Furthermore, the study contributes to the valorization of regional biodiversity and phytotherapy, offering a foundation for future research and the development of natural products for pharmaceutical application.
References
Alves, J. K. S. (2016). Análise fitoquímica e avaliação da atividade antimicrobiana de extratos vegetais [Dissertação de mestrado, Universidade Federal de Minas Gerais].
Amorim, L. S., et al. (2021). In vitro antibacterial and anti-inflammatory effects of Anacardium occidentale L. extracts and their toxicity on PBMCs and zebrafish embryos. Drug and Chemical Toxicology, 45(6).
Baptista, A. B. (2018). Extrato de folhas de caju (Anacardium occidentale L.) e de cajuí (Anacardium microcarpum D.): prospecção fitoquímica, atividade antioxidante, antimicrobiana e anti-inflamatória, in vitro e in vivo [Dissertação de mestrado, Universidade Federal de Viçosa]. https://locus.ufv.br/items/c70d9147-2340-45dc-822b-c6601761e47d
Barbosa-Filho, V. M. (2015). Avaliação do potencial antioxidante e antibacteriano de Anacardium microcarpum e sua toxicidade em diferentes modelos biológicos in vitro [Tese de doutorado, Universidade Federal de Santa Maria].
Braga, D. C., et al. (2018). Perfil de compostos fenólicos em pseudofrutos de clones de cajueiro anão-precoce por UPLC-QToF-MS. Anais do 58º Congresso Brasileiro de Química.
Brasil. (2009). MS elabora Relação de Plantas Medicinais de Interesse ao SUS. http://bvsms.saude.gov.br/bvs/sus/pdf/marco/ms_relacao_plantas_medicinais_sus_0603.pdf
Brasil. Ministério da Saúde. (2015). Política Nacional de Práticas Integrativas e Complementares no SUS – PNPIC-SUS (2ª ed., pp. 48–56). Editora MS-OS.
Chen, S., et al. (2023). A review of classification, biosynthesis, biological activities and potential applications of flavonoids. Molecules, 28(13), 4982. https://doi.org/10.3390/molecules28134982
Costa, N. B., et al. (2021). Obtenção do perfil químico de extratos das folhas do cajueiro (Anacardium occidentale) a partir de diferentes solventes. Research, Society and Development, 10(8), e40110817473. https://doi.org/10.33448/rsd-v10i8.17473
Maheshwaran, U., Sultana, S., Yousuf, S., & Rani, M. (2024). Phytochemical testing methodologies and principles for preliminary screening/qualitative testing. Asian Plant Research Journal, 12(5), 11–38.
Pinelo, M., et al. (s.d.). Extraction of antioxidant phenolics from almond hulls (Prunus amygdalus).
Rodrigues, A. R. S. P. (2023). Potencial antioxidante, antimicrobiano, anti-inflamatória e antifúngica da Anacardium occidentale (Linn): Revisão de literatura. Revista Colombiana de Ciencias Químico-Farmacéuticas, 52(1).
Rodrigues Júnior, J. G. (2022). Perfil fitoquímico, atividade antifúngica e toxicidade do extrato de Anacardium occidentale Linn. sobre Candida sp. [Trabalho de conclusão de curso, Universidade Estadual da Paraíba]. https://dspace.bc.uepb.edu.br/jspui/handle/123456789/28904
Santos, J. A. S., et al. (2018). Estudo do potencial antioxidante da Anacardium occidentale L. e determinação de seus compostos fenólicos. Diversitas Journal, 3(2). https://www.diversitasjournal.com.br/diversitas_journal/article/view/637
Santos, F. A. P., et al. (2019). Influência do solvente na extração dos compostos fenólicos e na atividade antioxidante de plantas medicinais. Revista Brasileira de Farmacognosia, 29(4), 519–528. https://doi.org/10.1016/j.bjp.2018.12.003
Santos, R. F., Silva, M. A., & Lima, J. M. (2018). Avaliação qualitativa e quantitativa de saponinas em plantas medicinais por testes preliminares e cromatografia em camada delgada. Revista Brasileira de Farmacognosia, 28(4), 507–513. https://doi.org/10.1016/j.bjp.2017.11.007
Silva, F. A., Bizerra, A. M. C., & Fernandes, P. R. D. (2018). Testes fitoquímicos em extratos orgânicos de Bixa orellana L. (Urucum). Holos, 34(2), 484–491. https://doi.org/10.15628/holos.2018.7063
Silva, A. P., Sousa, M. R., & Almeida, F. R. (2019). Avaliação fitoquímica e atividade antioxidante de extratos vegetais de Anacardium occidentale L. Revista Brasileira de Farmacognosia, 29(2), 236–244.
Souza, F. F., et al. (2016). Estudo fitoquímico e avaliação da atividade antioxidante de extratos de folhas de Anacardium occidentale L. Revista Brasileira de Plantas Medicinais, 18(1), 120–128. https://www.scielo.br/j/rbpm/a/X8fT4G8v9xX7H5yxS8zjN7p/
Souza, N. C., et al. (2017). Antioxidant and anti-inflammatory properties of Anacardium occidentale leaf extract. Evidence-Based Complementary and Alternative Medicine, 2017, 2787308. https://doi.org/10.1155/2017/2787308
Zhang, C., et al. (2023). Comprehensive review of recent advances in chiral A-ring flavonoid containing compounds: structure, bioactivities, and synthesis. Molecules, 28(1), 365. https://doi.org/10.3390/molecules28010365.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 João Luiz Ribeiro, Deny Alves Nogueira

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
