Evaluation of the in vitro susceptibility of melanized fungi from carnauba (Copernicia prunifera) to fluconazole
DOI:
https://doi.org/10.33448/rsd-v14i8.49461Keywords:
Broth Microdilution, Fungi, Mycoses, Fungal Pharmacoresistance.Abstract
Objective: To investigate the in vitro susceptibility of melanized fungal isolates from carnauba to fluconazole. Methods: Microdilution techniques were used in sterile 96-well plates, standardized by document M38-A3 from the Clinical and Laboratory Standards Institute, in order to determine the Minimum Inhibitory Concentration (MIC) of the samples. This evaluation is necessary to determine the lowest concentration capable of inhibiting the growth of the fungus, thus verifying whether the fungus is sensitive to the drug tested. 7 isolates of black fungi were evaluated, 5 coming from the trunk and 2 from carnauba leaves. They were considered sensitive to fluconazole when the MIC was ≤ 1 μg/mL. Results: Fluconazole inhibited the growth of 4 fungi tested, presenting a variable MIC in the range of 1 μg/mL to 32 μg/mL, although only one of them was sensitive with an MIC of 1 μg/mL. Conclusion: Although fluconazole inhibited the majority of tested isolates (57.14%), only one was sensitive, while the other 6 were resistant to the drug in vitro. This medication can be used in the treatment of black fungus infections in an ineffective way and not as a first-line treatment, and can be used in combination therapy with other antifungals. The importance of raising awareness among workers involved in Carnauba extraction is highlighted regarding the use of personal protective equipment, given the risk of exposure to microorganisms resistant to drugs such as fluconazole, which can worsen the treatment of melanized fungal infections.
References
Almeida, J. A. S., Feitosa, N. A., Sousa, L. C., Silva, R. N. O., Morais, R. F. & Monteiro, J. M. (2021). Use, perception, and local management of Copernicia prunifera (Miller) H. E. Moore in rural communities in the Brazilian Savanna. J Ethnobiol and Ethnomed, 17, 1. doi: 10.1186/s13002-021-00440-5.
Andrade, T. S. (1999). Susceptibilidade in vitro de agentes causadores de cromoblastomicose frente a diferentes antifúngicos. f. Dissertação (Mestrado) – Curso de Pós Graduação em Farmácia, Universidade de São Paulo. DOI: https://doi.org/10.11606/D.9.2019.tde-14102019-114206.
Badali, H., Najafzadeh, M. J., Esbroeck, M. V., Enden, E. V. D., Tarazooie, B. & Meis, J. F. G. M. V. (2010). The clinical spectrum of Exophiala jeanselmei, with a case report and in vitro antifungal susceptibility of the species. Med Mycol., 48(2), 318-327. doi: 10.1080/13693780903148353.
Belda, W. J., Passero, L. F. D, Carvalho, C. H. C, Mojica P. C. R. & Vale, P. A. (2023). Chromoblastomycosis: New Perspective on Adjuvant Treatment with Acitretin. Diseases, 11(4), 162. doi: 10.3390/diseases11040162.
Campos, T., Cosentino, C., Simioni, P. U. & Ugrinovich, L. (2020). Avaliação do Comportamento de Leveduras do gênero Candida a fármacos antifúngicos. RCI, 5:1. https://faculdadedeamericana.com.br/ojs/index.php/Ciencia_Inovacao/article/view/461.
CLSI - Clinical and Laboratory Standards Institute. (2017). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi. (3ed. CLSI standard M38. Wayne, PA: Clinica. https://clsi.org/standards/products/microbiology/documents/m38/.
Coelho, R. A, Brito-Santos, F., Figueiredo-Carvalho, M. H. G., Silva, J. V. D. S., Gutierrez-Galhardo, M. C. & Valle, A. C. F.(2018). .Molecular identification and antifungal susceptibility profiles of clinical strains Fonsecea spp. Isolated from patients with cromoblastomycosis in Rio de Janeiro, Brazil. Plos Negl Trop Dis. 212(7), e0006675. DOI: doi: 10.1371/journal.pntd.0006675.
Costa, F. F, Silva, N. M,. Voidaleski, M. F., Weiss, V. A., Moreno, L. F., & Schneider, G. X. (2020), Environmental prospecting of black yeast-like agents of human disease using culture-independent methodology. Sci Rep., 10 (1), 1-1. doi: 10.1038/s41598-020-70915-0.
Daboit, T. C., Magagnin, C. M., Heidrich, D., Antochevis, L.C, Vigolo, S. & Meirelles L. C. (2014). In vitro susceptibility of chromoblastomycosis agents to five antifungal drugs and to the combination of terbinafine and amphotericin B. Mycoses. 57(2), 116-20. doi: 10.1111/myc.12111.
Ferreira-Paim, K., Andrade-Silva, L., Mora, D. J., Lages-Silva, L., Pedrosa, A. L., & Silva, P. R. (2012). Antifungal susceptibility, enzymatic activity, PCR-fingerprinting and ITS sequencing of environmental Cryptococcus laurentii isolates from Uberaba, Minas Gerais, Brazil. Mycopathologia, 174(1), 41-52. doi: 10.1007/s11046-011-9500-0.
Marques, S. G., Silva C. M. P., Saldanha P. C., Rezende M. A., Vicente V. A. & Queiroz-Telles F. (2006) Isolation of Fonsecaea pedrosoi from the shell of the babassu coconut (Orbignya phalerata Martius) in the Amazon region of Maranhão Brazil. Nippon Ishinkin Gakkai Zasshi, 47(4), 305-311. doi: 10.3314/jjmm.47.305.
Milani, B., Dagne, D. A., Choi, H. L., Schito, M., Stone, H. A. (2023). Diagnostic capacities and treatment practices on implantation mycoses: Results from the 2022 WHO global online survey. Plos Negl Trop Dis., 17, 6. doi: 10.1371/journal.pntd.0011443.
Mouchalouat, M. F. (2008). Cromoblastomicose estudo de uma série de 14 casos atendidos no Instituto de Pesquisa Clínica Evandro Chagas – Fundação Oswaldo Cruz, Rio de Janeiro, de 1994 a 2005. 2008. f. Dissertação (Mestrado) - Curso de Mestrado em Pesquisa Clínica em Doenças Infecciosas, Instituto Nacional de Infectologia Evandro Chagas, Rio de Janeiro. https://www.arca.fiocruz.br/handle/icict/28073.
Najafzadeh, M. J., Sutton, D. A., Keisari, M. S., Zarrinfar, H., Hoog, G. S., & Chowdhary A. (2014). In vitro activities of eight antifungal drugs against 104 environmental and clinical isolates of Aureobasidium pullulans. Antimicrob Agents and Chemother, 58 (9), 5629-5631. doi: 10.1128/AAC.03095-14.
Neto, F. R. G. X., Crispim, F. S. P., & Braga, P. E. T. (2019) Processos produtivos de trabalhadores rurais no extrativismo da palha de carnaúba. Interações, 20(40), 1263-1273. Doi: https://doi.org/10.20435/inter.v20i4.1880.
Oedmann, A. F. B, Moraes, L. G. A., Freitas, M. C., Ferreira, B. A. S., & Mayer, N. (2024). Cromoblastomicose: estudo de 27 casos e revisão da literatura médica. Arch Health, [S. l.], 5, 3, p. e2091. https://doi.org/10.46919/archv5n3espec-404.
Pereira, G. B., Lizardo, C. A. R., Carvalho, L. C. S. & Souza, E. B. A. (2021). Ocorrência das infecções fúngicas subcutâneas em pacientes atendidos no laboratório de micologia médica do centro de pesquisa em medicina tropical. Braz J Dev., 7 (12), 121016–121029. DOI:https://doi.org/10.34117/bjdv7n12-732
Revankar, S.G., & Sutton, D.A. (2010). Melanized Fungi in Human Disease. Clin Microbiol Rev., 23 (4), 884-928. doi: 10.1128/CMR.00019-10.
Pereira, A. S. et al. (2018). Metodologia da pesquisa científica. [free ebook]. Santa Maria. Editora da UFSM.
Santos, A. K. O., Ferreira, C., Quadros, R. M., Miletti, L. C., & Ramos, C. J. R. (2020). Fungos causadores de micoses cutâneas em trabalhadores rurais da região da AMURES, Santa Catarina, Brasil. Rev Bras Hig Sanid Anim., 14, 4, 1-11. 1. http://dx.doi.org/10.5935/1981-2965.20200046.
Shenoy, M. M., Girisha, B. S. & Krishna S. (2023). Chromoblastomycosis: A Case Series and Literature Review. Indian Dermatol Online J., 14(5), 665-669. doi: 10.4103/idoj.idoj_292_23.
Silva, C. M., Rocha, R. M., Moreno, J. S., Branco, M. R., Silva, R. R., & Marques, S. G. (1995). O babaçu (Orbignya phalerata martins) como provável fator de risco de infecção humana pelo agente da cromoblastomicose no Estado do Maranhão, Brasil. Rev Soc Bras Med Trop., 28(1), 49-52. DOI: https://doi.org/10.1590/S0037-86821995000100009.
Smith, D. J., Queiroz-Telles, F., Rabenja, F. R., Hay, R., Bonifaz, A. & Grijsen, M. L. (2024). A global chromoblastomycosis strategy and development of the global chromoblastomucosys working group. Plos Negl Trop Dis, 18, 10. doi: 10.1371/journal.pntd.0012562.
Vitale, R. G., Perez-Blanco, M. & De Hoog, G. S. (2009). In vitro activity of antifungal drugs against Cladophialophora species associated with human chromoblastomycosis. Med Mycol., 47 (1), 35-40. doi: 10.1080/13693780802566333.
Voidaleski, M. F., Gomes, R. R., Azevedo, C. M. P., Lima B. F. S, Costa F. F. & Bombassaro A. (2020). Environmental Screening of Fonsecaea Agents of Chromoblastomycosis Using Rolling Circle Amplification. J Fungi., 6(4):290. doi: 10.3390/jof6040290.
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