Deficiencia de tiamina inducida por amprolio en ratones hembra: Papel del estrés oxidativo y la inflamación
DOI:
https://doi.org/10.33448/rsd-v14i6.49065Palabras clave:
Señalización celular, MAPK, Comportamiento, Diferencias sexuales, Trolox, Dimetilsulfóxido.Resumen
Los modelos experimentales de deficiencia de tiamina (DT) se han centrado principalmente en roedores machos, lo que significa que los efectos de la DT en hembras y la patogénesis asociada con trastornos neurológicos siguen siendo desconocidos. El objetivo de este artículo fue presentar una investigación sobre los efectos de la DT con amprolio en ratones hembra, evaluando los efectos metabólicos y conductuales, así como la modulación de la fosforilación de ERK1/2 en la corteza cerebral y el tálamo. Además, utilizamos el antioxidante Trolox y el antiinflamatorio dimetilsulfóxido para investigar el papel del estrés oxidativo y la neuroinflamación en este proceso. Los animales fueron expuestos a una dieta deficiente en tiamina con la administración adicional de amprolio (60 mg/kg) durante 20 días. Tras el tratamiento, observamos una reducción del consumo de alimento y del peso corporal de los animales, con una disminución de la coordinación motora y la actividad exploratoria, y, paralelamente, un aumento de la fosforilación de ERK1/2, tanto en la corteza cerebral como en el tálamo en los animales deficientes. Los animales deficientes que recibieron Trolox o dimetilsulfóxido presentaron una atenuación de estos efectos, con el mantenimiento de la coordinación motora y un bloqueo total de la activación de ERK1/2. Los resultados mostraron que las hembras de ratón podrían utilizarse como un modelo válido de DT, compatible con otros métodos, mostrando importantes cambios neurológicos. Este estudio demostró que, en las hembras, la DT también implica mecanismos de estrés oxidativo e inflamación, responde positivamente y puede utilizarse como modelo animal.
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Derechos de autor 2025 Mirian Pereira da Silva; Francisco Wanderson Bizerra Lima; Adha Gabriela Santos Moura; Julia Paiva Nunes; Clarissa Amorim Silva de Cordova; Fabiano Mendes de Cordova
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
Los autores que publican en esta revista concuerdan con los siguientes términos:
1) Los autores mantienen los derechos de autor y conceden a la revista el derecho de primera publicación, con el trabajo simultáneamente licenciado bajo la Licencia Creative Commons Attribution que permite el compartir el trabajo con reconocimiento de la autoría y publicación inicial en esta revista.
2) Los autores tienen autorización para asumir contratos adicionales por separado, para distribución no exclusiva de la versión del trabajo publicada en esta revista (por ejemplo, publicar en repositorio institucional o como capítulo de libro), con reconocimiento de autoría y publicación inicial en esta revista.
3) Los autores tienen permiso y son estimulados a publicar y distribuir su trabajo en línea (por ejemplo, en repositorios institucionales o en su página personal) a cualquier punto antes o durante el proceso editorial, ya que esto puede generar cambios productivos, así como aumentar el impacto y la cita del trabajo publicado.
