Deficiência de tiamina induzida por amprólio em camundongos fêmeas: Papel do estresse oxidativo e da inflamação
DOI:
https://doi.org/10.33448/rsd-v14i6.49065Palavras-chave:
Sinalização celular, MAPK, Comportamento, Diferenças sexuais, Trolox, Dimetilsulfóxido.Resumo
Modelos experimentais de deficiência de tiamina (DT) têm se desenvolvido principalmente em roedores machos, fazendo com que os efeitos da DT em fêmeas e a patogênese associada a distúrbios neurológicos permanecem desconhecidos. O objetivo deste artigo foi apresentar uma investigação sobre os efeitos da DT com amprólio em camundongos fêmeas, avaliando efeitos metabólicos e comportamentais, bem como a modulação da fosforilação de ERK1/2 no córtex cerebral e no tálamo. Além disso, utilizamos o antioxidante Trolox e o anti-inflamatório dimetilsulfóxido para investigar o papel do estresse oxidativo e da neuroinflamação nesse processo. Os animais foram expostos a uma dieta deficiente em tiamina com a administração adicional de amprólio (60 mg/kg) por 20 dias. Após o tratamento, observamos redução no consumo alimentar e no peso corporal dos animais, com diminuição da coordenação motora e da atividade exploratória e, paralelamente, aumento da fosforilação de ERK1/2, tanto no córtex cerebral quanto no tálamo dos animais deficientes. Animais deficientes que receberam Trolox ou dimetilsulfóxido apresentaram atenuação desses efeitos, com manutenção da coordenação motora e bloqueio total da ativação de ERK1/2. Os resultados mostram que camundongos fêmeas podem ser usados como um modelo válido de DT, compatível com outros métodos, apresentando alterações neurológicas importantes. Este estudo evidencia que, em fêmeas, a DT também envolve mecanismos de estresse oxidativo e inflamação, respondem positivamente e podem ser usadas como modelo animal.
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Copyright (c) 2025 Mirian Pereira da Silva; Francisco Wanderson Bizerra Lima; Adha Gabriela Santos Moura; Julia Paiva Nunes; Clarissa Amorim Silva de Cordova; Fabiano Mendes de Cordova
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Autores que publicam nesta revista concordam com os seguintes termos:
1) Autores mantém os direitos autorais e concedem à revista o direito de primeira publicação, com o trabalho simultaneamente licenciado sob a Licença Creative Commons Attribution que permite o compartilhamento do trabalho com reconhecimento da autoria e publicação inicial nesta revista.
2) Autores têm autorização para assumir contratos adicionais separadamente, para distribuição não-exclusiva da versão do trabalho publicada nesta revista (ex.: publicar em repositório institucional ou como capítulo de livro), com reconhecimento de autoria e publicação inicial nesta revista.
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