Optimización de la mezcla de solventes para la extracción de compuestos fenólicos con actividad antioxidante y fitotoxicidad de hojas de Myrcia fallax utilizando el I-optimal design
DOI:
https://doi.org/10.33448/rsd-v14i9.49574Palabras clave:
M.fallax, I-optimal, Actividad antioxidante y fitotoxicidad.Resumen
El objetivo de este estudio fue, a través de un diseño I-óptimo, mejorar la extracción del contenido fenólico total (CPT) y los compuestos flavonoides totales (CFT) de las hojas de Myrcia fallax (A. Rich) DC a partir de una mezcla de tres solventes de etanol, metanol y agua (máximo 50%) para obtener los mejores resultados de rendimiento, antioxidantes y fitotóxicos. El metanol proporcionó mayores rendimientos porcentuales de extracción, mientras que para el contenido fenólico total (CPT) y el contenido flavonoides totales (CFT), se dieron mejores valores al metanol-agua. Además, el etanol puro tuvo mejores resultados con respecto a la actividad antioxidante utilizando los métodos DPPH y ABTS. Para la evaluación de la actividad fitotóxica, se indicaron extractos de metanol-agua debido a su capacidad para inhibir la longitud de la radícula y estimular el crecimiento del hipocótilo en comparación con el control. Luego, el diseño I-óptimo nos permitió correlacionar los extractos de Myrcia fallax con sus actividades biológicas a partir de mezclas de solventes de diferente polaridad.
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