Controle biológico da doença da mancha negra induzida por Alternaria alternata em Dioscorea opposita utilizando Bacillus sp. E-Do8 endofítico
DOI:
https://doi.org/10.33448/rsd-v15i3.50706Palavras-chave:
Dioscorea alternata, Bactéria endofítica, Bacillus, Doença da mancha negra, Alternaria alternata, Surfactina.Resumo
As bactérias endofíticas de plantas desempenham papéis cruciais na prevenção e no controle de doenças em culturas agrícolas. O inhame (Dioscorea opposita), um importante tubérculo e raiz cultivada, é conhecido por seus ricos valores hortícolas e medicinais. No entanto, a mancha-preta é relativamente prevalente durante seu cultivo, e não há relatos sobre o uso de endófitos para controlar essa doença. Neste contexto, este artigo teve como objetivo apresentar um estudo sobre o controle biológico da mancha negra causada por Alternaria alternata em Dioscorea opposita utilizando a bactéria endofítica Bacillus sp. E-Do8. Uma bactéria endofítica que antagonizou fortemente Alternaria alternata, o patógeno causador da mancha-preta do inhame, foi isolada de folhas de inhame Tiegun e identificada como uma cepa de Bacillus, denominada E-Do8. Investigações posteriores revelaram que o caldo de fermentação de E-Do8 inibiu significativamente a germinação de esporos e o crescimento micelial de A. alternata, e o micélio apresentou espessura irregular, inchaço e emaranhamento irregular. Além disso, a capacidade de A. alternata de penetrar celofane foi reduzida quando cultivada com o caldo de fermentação de E-Do8, o que também inibiu significativamente a patogenicidade de A. alternata. Além disso, o sistema UHPLC-Orbitrap Exploris 240 foi empregado para analisar os ingredientes ativos no caldo de fermentação E-Do8 que inibem A. alternata, e constatou-se que E-Do8 produziu apenas compostos do tipo surfactina, surfactina C14 e surfactina.
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Copyright (c) 2026 Louis Antoniel Joseph, Manoucheca Jean, Bento Gil Uane, Frantzdy Luc, Meque Samuel Tivane, Kerley-Vivaldi Jean, Inocêncio Oliveira Mulaveia
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