Comparative evaluation of the aqueous and ethanolic extracts of Erythrina mulungu: Chemical composition and biological activities
DOI:
https://doi.org/10.33448/rsd-v15i2.50640Keywords:
Erythrina mulungu, Plant extracts, Bioactive compounds.Abstract
Plant extracts are widely used as sources of bioactive compounds, especially phenols, flavonoids, and alkaloids, whose extraction depends directly on the type of solvent employed. This study aimed to compare the aqueous and ethanolic extracts obtained from the bark of Erythrina mulungu, evaluating their chemical and functional characteristics through High-Performance Liquid Chromatography (HPLC), Fourier Transform Infrared Spectroscopy (FTIR), total phenolic content, antimicrobial activity, and antioxidant activity analyses. The aqueous extract showed lower chemical diversity and reduced bioactivity, with a phenolic content of 7.64 mg GAE/g, antimicrobial halos smaller than 14 mm, and 14.70% inhibition in the DPPH assay. In contrast, the ethanolic extract exhibited a more complex composition, with chromatographic peaks associated with erythrinic alkaloids and lipophilic phenols, as well as additional FTIR bands consistent with carbonyls, ethers, and amines. This extract presented a phenolic content of 729.68 mg GAE/g, halos of 21.50 mm (E. coli) and 35.75 mm (S. aureus), and high antioxidant activity (92.82%). The results demonstrate that ethanol promotes a more efficient extraction of bioactive metabolites, whereas the aqueous extract shows a more restricted composition. Thus, solvent selection directly influences the chemical profile and biological performance of Erythrina mulungu extracts, reinforcing the importance of choosing an appropriate extraction method in phytochemical studies.
References
Alara, O. R., Abdurahman, N. H., & Ukaegbu, C. I. (2021). Extraction of phenolic compounds: A review. Current Research in Food Science, 4, 200–214.
Alves, V. F., Figueiredo, R. D., Cavalcanti, Y. W., & Padilha, W. W. N. (2019). Atividade antimicrobiana de plantas medicinais indicadas para uso no Sistema Único de Saúde. Revista Cubana de Estomatología, 56(4), 1–16.
Amorim, J., Borges, M. C., Fabro, A. T., Contini, S. H. T., Valdevite, M., Pereira, A. M. S., & Carmona, F. (2019). The ethanolic extract from Erythrina mulungu Benth. flowers attenuates allergic airway inflammation and hyperresponsiveness in a murine model of asthma. Journal of Ethnopharmacology, 243, 112108.
Andrews, J. M. (2001). Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy, 48(Suppl. 1), 5–16.
Awad, A. M., Kumar, P., Ismail-Fitry, M. R., Jusoh, S., Aziz, M. F. A., & Sazili, A. Q. (2022). Overview of plant extracts as natural preservatives in meat. Institute of Food Science & Technology.
Aryal, S., Baniya, M. K., Danekhu, K., Kunwar, P., Gurung, R., & Koirala, N. (2019). Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants, 8(96), 1–13.
Bezerra, J. W. A., Coronel, C., Gomez, M. C. V., Rolón, M., Nunez, C. V., Silva, D. R., Silva, L. A., Rodrigues, F. C., Boligon, A. A., Souza, M. A., Linhares, K. V., Silva, M. A. P., & Morais-Braga, M. F. B. (2019). Evaluation of antiparasitary, cytotoxic and antioxidant activity and chemical analysis of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae). South African Journal of Botany, 124, 546–555.
BrCAST–EUCAST. (2021). Guia de leitura: Método de disco-difusão para teste de sensibilidade aos antimicrobianos (Versão 8.0).
Cardoso, A. P. M. N. (2021). Uso do bioestimulante à base de algas marinhas e água residuária de piscicultura na produção de mudas de mulungu (Erythrina velutina Willd) em solo de área degradada (Dissertação de Mestrado). Instituto Federal do Rio Grande do Norte.
Cavalcante, P. V. D. (2018). Desenvolvimento e caracterização de filme ativo antimicrobiano de amido de araruta/quitosana incorporado com óleo essencial de Thymus vulgaris *(Dissertação de Mestrado). Universidade Federal de Pernambuco.
Cavallieri, K. N. (2019). Estudo químico e avaliação biológica dos alcaloides presentes em Erythrina mulungu (Fabaceae) [Dissertação de Mestrado, Universidade Federal de Uberlândia].
Clinical and Laboratory Standards Institute. (2015). M02-A12: Performance standards for antimicrobial disk susceptibility tests. CLSI.
Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582.
Da Silva Mota, G., Nery, F. C., da Silva Araujo, E., et al. (2025). Characterization of cork features of Erythrina mulungu and Enterolobium gummiferum in view of a potential valorization and conservation of forest species in the Cerrado bioma. European Journal of Wood and Wood Products, 83(2), 1–12.
De Lima, A. C. A., Brito, A. M. S. S., Caetano, V. F., & Vinhas, G. M. (2025). Incorporation of cashew bark extract and gamma irradiation effects on biodegradable bacterial cellulose/kappa-carrageenan film. Express Polymer Letters, 19, 610–627.
Do Nascimento, H. A. (2022). Produção de biopolímero de celulose bacteriana aditivado com antioxidante natural (Tese de Doutorado). Universidade Federal de Pernambuco.
El-Saadony, M. T., Saad, A. M., Mohammed, D. M., Alkafaas, S. S., Abd El-Mageed, T. A., Ahmed, A. E., Algopishi, U. B., Abu-Elsaoud, A. M., Mosa, W. F. A., El-Tarabily, K. A., & AbuQamar, S. F. (2025). Plant bioactive compounds: Extraction, biological activities, immunological, nutritional aspects, food application, and human health benefits—A comprehensive review. Frontiers in Nutrition, 12, 1659743.
Ferreira Neto, J. P. (2017). Atividade citotóxica, avaliação fitoquímica e atividade antimicrobiana de plantas medicinais de Pernambuco [Dissertação de Mestrado, Universidade Federal de Pernambuco].
Herlina, T., Akili, A. W. R., Nishinarizki, V., Hardianto, A., & Latip, J. B. (2025). Review on antibacterial flavonoids from genus Erythrina: Structure–activity relationship and mode of action. Heliyon, 11(1).
Jafari, M. S., Ghadami, S., Mantripragada, S., Dellinger, K., Alston, J. R., & Lajeunesse, D. R. (2025). Facile and green carboxylation of never-dried bacterial cellulose produced from low-cost substrates: Structural characterization and copper binding performance. Materials Advances, 6(1), 8936–8951.
Joselin, J., Benila, B. S., Brintha, T. S. S., & Jeeva, S. (2025). Phytochemical profiling, FT-IR spectroscopy, and antioxidant evaluation of select Lamiaceae species. Intelligent Pharmacy, 3(2), 111–117.
Lima, N. F., Maciel, G. M., Fernandes, I. A. A., & Haminiuk, C. W. I. (2022). Adsorção de fenólicos em celulose bacteriana otimizada. Revista Brasileira de Pesquisa em Alimentos, 13(2), 45–58.
McDonnell, G., & Russell, A. D. (1999). Antiseptics and disinfectants: Activity, action, and resistance. Clinical Microbiology Reviews, 12(1), 147–179.
Mungwari, C. P., King'ondu, C. K., Sigauke, P., & Obadele, B. A. (2025). Conventional and modern techniques for bioactive compounds recovery from plants: Review. Scientific African, 27, e02509. https://doi.org/10.1016/j.sciaf.2024.e02509
Pereira, A. S. et al. (2018). Metodologia da pesquisa científica. (Free ebook). Santa Maria. Editora da UFSM.
Poffo Spézia, F., Siebert, D., Tenfen, A., Cordova, C. M. M., Alberton, M. D., & Guedes, A. (2020). Avaliação da atividade antibacteriana de plantas medicinais de uso popular: Alternanthera brasiliana, Plantago major, Arctostaphylos uva-ursi e Phyllanthus niruri. Revista Pan-Amazônica de Saúde, 11.
Rambo, D. F. (2011). Determinação de parâmetros de controle de qualidade para Erythrina verna Vell. (Dissertação de Mestrado). Universidade Federal do Rio Grande do Sul.
Ramos, R. M., Feitosa, J. M., Da Silva Lima, V., Da Conceição Santos, R., De Oliveira, A. M., Soares, L. A. L., & Da Silva, G. C. (2020). Estudo comparativo da composição fitoquímica, citotoxicidade e potenciais antioxidante e fotoprotetor da casca e folha de Erythrina velutina. Brazilian Journal of Development, 6(6), 33140–33158.
Risemberg, R. I. C., Wakin, M., & Shitsuka, R. (2026). A importância da metodologia científica no desenvolvimento de artigos científicos. E-Acadêmica. 7(1), e0171675.
Santiago, I. F., Damasceno, H. C. M. C., & Cavelet, L. C. (2022). Perfil farmacológico da Erythrina velutina no transtorno de ansiedade. Revista Ibero-Americana de Humanidades, Ciências e Educação, 8(10), 1711–1720.
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144–158.
Silverstein, R. M., Webster, F. X., Kiemle, D. J., & Lipkins, K. (2014). Spectrometric identification of organic compounds (8ª ed.). Wiley.
Soneguete, G. C., Gonçalves, M. C., Silva, R. A., Lima, A. M. F., & Pereira, A. C. S. (2019). Avaliação da atividade antioxidante de extratos de Protium spp. pelo método DPPH. Anais do Congresso Brasileiro de Química, 62.
Tondi, G., Link, M., Oo, C. W., & Petutschnigg, A. (2015). A simple approach to distinguish classic and formaldehyde-free tannin-based rigid foams by ATR FT-IR. Journal of Spectroscopy, 2015(1), 1–8.
Valgas, C., Souza, S. M., Smânia, E. F., & Smânia, A. (2007). Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology, 38(2), 369–380.
Veber, J., Petrini, L. A., Andrade, L. B., & Siviero, J. (2019). Determinação dos compostos fenólicos e da capacidade antioxidante de extratos aquosos e etanólicos de Jambolão (Syzygium cumini). Revista Brasileira de Plantas Medicinais.
Wang, J., Tavakoli, J., & Tang, Y. (2019). Bacterial cellulose production, properties and applications with different culture methods – A review. Carbohydrate Polymers, 219, 63–76. https://doi.org/10.1016/j.carbpol.2019.05.008
Xing, T.-Q., Zhao, T.-F., Hu, C.-S., Cao, X.-F., Sun, S.-N., Shen, X.-J., Wen, J.-L., Yuan, T.-Q., & Sun, R.-C. (2024). Ultrafast surface esterification of cellulosic materials in aqueous media at room temperature. Cellulose, 31(1), 10241–10252.
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