Ratas hipertensas ovariectomizadas submetidas a exercício e terapia estrogênica apresentam níveis melhorados de receptores de angiotensina na aorta
DOI:
https://doi.org/10.33448/rsd-v14i12.50241Palavras-chave:
Sistema renina-angiotensina (SRA), Óxido nítrico sintase endotelial (eNOS), Superóxido Dismutase 2 (SOD 2).Resumo
O sistema renina-angiotensina (SRA) e o sistema antioxidante exercem funções integradas na regulação cardiovascular, especialmente diante do treinamento físico e de alterações nos níveis de estrogênio. Este estudo teve como objetivo investigar se o treinamento modula receptores do SRA e proteínas antioxidantes de forma semelhante à terapia com 17β-estradiol (E2) na aorta de ratas espontaneamente hipertensas ovariectomizadas (SHR). Os animais foram distribuídos em cinco grupos: Sham (SH), Ovariectomizado (OVX), OVX+E2 (OE2), OVX+natação (OSW) e OVX+E2+natação (OE2+SW). A terapia hormonal consistiu em 5 µg de E2, três vezes por semana; o treinamento em natação foi realizado uma hora por dia, cinco vezes por semana. Dois dias após o último procedimento, avaliou-se a pressão arterial sistólica. Amostras de aorta foram analisadas por western blot para os receptores AT1R, AT2R e Mas, além de eNOS, proteína quinase B total e fosforilada (p-AktSer473), SOD2 e β-actina. Observou-se aumento de AT1R apenas no grupo OVX, enquanto AT2R e Mas aumentaram nos grupos OSW e OE2+SW. A terapia com E2 elevou eNOS e SOD2 nos grupos SH, OE2 e OE2+SW, padrão também observado para p-AktSer473. Os achados sugerem que intervenções físicas e hormonais estimulam vias protetoras do SRA, especialmente p-AktSer473-eNOS e SOD2, favorecendo vasodilatação e capacidade antioxidante em condições de deficiência estrogênica.
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