Comparative theoretical-experimental study applied to the analysis of the gene expression of DNA forms in arthropods and mammalian cells infected by Dengue Vírus
DOI:
https://doi.org/10.33448/rsd-v9i11.10687Keywords:
DNA forms; Dengue vírus; Persistent infection.Abstract
Objective: A study with a theoretical-experimental approach applied to the gene expression of DENV DNA forms in mammalian cells, compared to the presence of fragments originating from viral RNA described in the literature in arthropod cells, experimentally infected by DENV. Methodology: This is a bilateral study, of a theoretical-experimental nature, with a primary intervention basis, carried out in vitro, under a comparative technical approach. Laboratory techniques for gene expression by conventional PCR, verification procedures for analysis and literature data records were obtained and processed at the Viral Biogenesis Laboratory, linked to the Arbovirology and Hemorrhagic Fevers Sector of the Evandro Chagas Institute. Results: Experimental analysis indicated the presence of DENV-4 DNA forms in cells persistently infected by inoculation at 72 hpi. As a comparative function, C6/36 cells presented viral DNA sequences incorporated in regions of LTR retrotransposons, thus allowing to suggest that most integration events are mediated by reverse transcriptases derived from retrotransposons. Conclusion: Given the results demonstrated, it becomes evident the need for studies that characterize the ability of viral RNA to be converted into DNA forms, as well as the influence of this characteristic on the permanence of these fragments after infection, to which proteins these genes belongs, how these genes modify and interact in viral replication and the relationship of pathogenicity and tropism associated with post-mutation protein molecular plasticity, during viral replication.
References
Brazil. Ministry of Health. Health Surveillance Secretaria (2010). Dengue Epidemiological Report - Analysis of situations and trends.
Bäck, A. T & Lundkvist, A. (2013). Dengue viruses - an overview. Infection ecology and epidemiology. Virus research.
Bhatt, S., Gething, P. W., Brady, A. C., Messina, J. C. (2013). The global distribution and burden of dengue. Nature (496), 504-507.
Blair, C. D., Adelman, Z. N., Olson, K. E. (2000) Molecular strategies for interrupting arthropod borne virus transmission by mosquitoes, Clin. microbiol. rev., 651-61.
Casseb, A. R., Casseb, L. M. N., Silva, S. P., Vasconcelos, P. F. C. (2013). Arbovirus: important zoonosis in the Brazilian Amazon. Veterinary and zootechnics, 20 (3), 9-21.
Chang, G. J. (1997). Molecular biology of dengue virus: dengue and dengue hemorrhagic fever. New York: Cab international, 175-97.
Chambers, T. J. (1990). Flavivirus genome organization, expression, and replication. Annual review of microbiology, Palo alto, (44): 649-88.
Dias, L., Almeida, S., Haes, T., Mota, L., Roriz-filho, J. (2010). Dengue: transmission, clinical aspects, diagnosis and treatment. Medicine, 43, 143-52.
Donalisio, M. R., Freitas. A. R. R., Zuben, A. P. B. V. (2017). Emerging arboviruses in Brazil: Challenges for the clinic and implications for public health, Revista de saúde Pública. 51, 30-40.
Geuking, M. B., Weber, J., Dewannieux, M., Gorelik, E., Heidmann, T., Hengartner, H., Zinkernagel, R. M., Hengartner, J. (2009) The recombination of the retrotransposon virus and exogenous rna results in the integration of the non-retroviral science cdna, 323, 393 - 396.
Guy, B., Barrere, B., Mallinowski, C., Saville, M., Teyssou, R., Lang, J. (2011), Fram research to phase iii: pre-clinical, industrial and and clinical development of the sanofi pasteur tetravalent dengue vaccine, vaccine, (29), 7229 -41.
Higgs, S. (2016). Zika virus: emergence and emergency. vector-borne zoonotic dis. 16: 75-76.
Horie, M., Honda, H., Suzuki, Y., Kobayashi, Y., Daito, T., Oshida, K., Ikuta, P., Jern, T. Gojobori, J. M., Coffin, K. Tomonaga (2010). endogenous non-retroviral rna virus elements in mammalian genomes, nature, 463, 84-88.
Hapuarachci, C., Helen, O., Thein, T. L., Tan, K., Lee, K S., Leo, Y. S. (1997). clinical-genetic evaluation of an encephalitic dengue virus 4 associated with multiple organ development, journal of clinical virology, 57 (9) 91-94.
Klenerman, P., hengartner, H., zinkernagel, R. M. (1997) a non-retroviral rna virus persist in dna form, nature, 104 (390) 298-301.
Melo, K. F L. (2018). expression profile of microwaves 15 and 16 in human cells during experimental dengue virus infection, master's thesis, Graduate Program in Virology, Instituto Evandro Chagas.
Modis, Y., Ogata, S., Clements, D., Harrison, S. C. (2004), Structure of the dengue virus envelope protein after membrane fusion. Nature, 22 (18) 427-37
Moya, A., & Holmes, E. C., González-candelas, F. (2004). The population genetics and evolutionary epidemiology of RNA viruses. nature reviews microbiology, 2 (4), 279–288.
Nag, D., K., Brecher, M., Kramer, L. D. (2016). DNA forms of arboviral RNA genomes are generated following infection in mosquito cell cultures. virology, 498, 164–171.
Nag, D. K., & Kramer, L. D. (2017), Patchy DNA forms of the Zika Virus RNA genome are generated following infection in mosquito cell cultures and in mosquitoes, journal of general virology. 98.
Pereira, A. S. (2018). Metodologia da pesquisa científica. Santa Maria. Ed. UAB. Disponível em:https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.
Sohler, M., & Rosadas, C. (2008), Advances and new insights in the neuropathogenesis of dengue infection, arq. neurophysic. 4(16), 698-703.
Weaver, S., & Reisen, W. (2010). Present and future arboviral threats, antivir, res., 85 (11) 328-345.
Xavier, A., Freitas, M., Loureiro, F., Borghi, D., Kanaan, S. (2014). Clinical manifestations of dengue: laboratory diagnosis, JBM, 2 (6) 102-08
Zhao, B., Prince, G., Horswood, R., Eckells, K., Summers, P., Chanock, R., Lai, C. J. (1987), Expression of dengue virus structural proteins and nonstructural protein NS1 by a recombinant vaccinia virus, j. virol. 4 (17) 4019-4022.
Zhdanov, M. (1985). Integration of viral genomes, Nature, 256 (16), 471-473.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Murilo Tavares Amorim; Jardel Fábio Lopes Ferreira; Francisco Canindé Ferreira de Luna; Walter Félix Franco Neto; Karla Fabiane Lopes de Melo; Ana Paula Sousa Araújo; Gleissy Adriane Lima Borges; Samir Mansour Moraes Casseb; Ana Cecília Ribeiro Cruz; Gustavo Moraes Holanda
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
