Accuracy of ultrasound for detecting liver metastasis xenografts in nude mice




Ultrasound; nude mice; preclinical model; liver metastasis; orthotopic xenograft.


Nude mice are the usual animal model for studying orthotopically implanted human tumors, and ultrasound imaging has emerged as a viable method for measuring tumors implanted orthotopically. The aim of this study was to evaluate ultrasound findings of liver tumors implanted into mice using a 13-MHz transducer and to correlate these findings with gross pathology and histological examinations. Tumor samples from liver metastases were obtained surgically from patients, and 1-mm³ fragments were implanted into the liver parenchyma of 38 nude mice. Mice were imaged monthly by ultrasound until sacrifice. Of the 38 mice implanted with tumor fragments, 11 developed tumors. Ultrasound detected nodular lesions in the 11 macroscopically positive animals and was able to identify the features of the engrafted tumors. Ultrasound imaging is a viable and noninvasive method for evaluating the liver parenchyma of nude mice, and showed 100% sensitivity and specificity in detecting and characterizing lesions.


Ayers GD, McKinley ET, Zhao P, et al. (2010). Volume of preclinical xenograft tumors is more accurately assessed by ultrasound imaging than manual caliper measurements. J Ultrasound Med. 29(6):891-901.

Cheung AM, Brown AS, Hastie LA, et al. (2005). Three-dimensional ultrasound biomicroscopy for xenograft growth analysis. Ultrasound Med Biol. 31(6):865-870.

Coatney RW. (2001). Ultrasound imaging: principles and applications in rodent research. ILAR J. 42(2):233-247.

Czarnota GJ, Kolios MC, Abrahan J, et al. (1999). Ultrasound imaging of apoptosis: high-resolution non-invasive monitoring of programmed cell death in vitro, in sito and in vivo. Br J Cancer. 81(3):520-527.

Frankel, J.C. (2014). The littlest patient. Science. 346(6205):24-27.

Graham KC, Wirtzfeld LA, MacKenzie LT, et al. (2005). Three-dimensional high-frequency ultrasound imaging for longitudinal evaluation of liver metastases in preclinical models. Cancer Res. 65(12):5231-5237.

Hidalgo M, Amant F, Biankin AV et al. (2014). Patient-derived xenograft models: an emerging platform for translational cancer research. Cancer Discov. 4(9):998-1013.

Hoffman RM. (1999). Orthotopic metastatic mouse models for anticancer drug discovery and evaluation: a bridge to the clinic. Invest New Drugs.17(4):343-359.

Hoffman RM. (2015). Patient-derived orthotopic xenografts: better mimic of metastasis than subcutaneous xenografts. Nat Rev Cancer. 15(8):451-452.

Kubota T. (1994). Metastatic models of human cancer xenografted in the nude mouse: the importance of orthotopic transplantation. J Cell Biochem. 56(1):4-8.

Murakami T, Zhang Y, Wang X, et al. (2016). Orthotopic implantation of intact tumor tissue leads to metastasis of OCUM-2MD3 human gastric cancer in nude mice visualized in real time by intravital fluorescence imaging. Anticancer Res. 36(5):2125-2130.

Oh, B.Y.; Hong, H.K., Lee, W.Y. & Cho, Y.B. (2017). Animal models of colorectal cancer with liver metastasis. Cancer Lett. 387:114-120.

Pereira, A.S.; Shitsuka, D.M.; Parreira, F.J. & Shitsuka, R. (2018). Metodologia da pesquisa científica. [e-book]. Ed. UAB/NTE/UFSM, Santa Maria/RS. Available from:

Tanaka N, Dalton N, Mao L, et al. (1996). Transthoracic echocardiography in models of cardiac disease in the mouse. Circulation. 94(5):1109-1117.

Turnbull, D.H., Bloomfield, T.S., Foster, F.S. & Joyner, A.L. (1995). Ultrasound backscatter microscope analysis of early mouse embryonic brain development. Proc Natl Acad Sci. 92:2239-2243.

Turnbull DH, Ramsay JA, Shivji GS, et al. (1996). Ultrasound backscatter microscope analysis of mouse melanoma progression. Ultrasound Med Biol. 22(7):845-853.




How to Cite

ROQUE, C. C. de T. A.; LIMA, E. N. P.; CHOJNIAK, R.; LIMA, B. de T. A. R.; SANTOS, T. G. dos. Accuracy of ultrasound for detecting liver metastasis xenografts in nude mice. Research, Society and Development, [S. l.], v. 8, n. 8, p. e20881181, 2019. DOI: 10.33448/rsd-v8i8.1181. Disponível em: Acesso em: 23 feb. 2024.



Agrarian and Biological Sciences