Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade

Authors

DOI:

https://doi.org/10.33448/rsd-v10i1.11845

Keywords:

Medicinal plant; Vascular smooth muscle; Solanaceae; L-type calcium channels.

Abstract

Previous results showed the ethyl acetate phase (SP-AcOEt), obtained from aerial parts of Solanum paludosum, relaxed the aorta isolated in the endothelium-dependent and -independent manner. The vasorelaxant effects of SP-AcOEt was not characterized on aorta rings endothelium-denuded, thus this work aimed to elucidate the mechanisms endothelium-independent vasorelaxation on rat isolated aorta. The aorta was isolated from Wistar rats and mounted in glass baths containing 6 mL of normal Krebs physiological solution with pH at 7.4. The preparation was maintained at 37ºC and bubbled continuously with a mixture of 95% O2 and 5% CO2. Aortic rings were maintained for 1 hour by a resting tension of 1g and next were contracted with phenylephrine after the sustained contraction ACh was added to access the integrity of the endothelium. SP-AcOEt relaxed pre-contracted aorta by KCl-30mM or -80mM in a similar manner, suggesting blockade CaV, but not channel-K+ participating. SP-AcOEt also inhibited the contraction induced by CaCl2 and relaxed pre-contracted aorta by (±)-BayK8644 (EC50 = 16.9±1.3 μg/mL), which confirms the involvement of L-type CaV blockade. SP-AcOEt presented vasorelaxation endothelium-independent that involves L-type Cablockade.

Author Biographies

Joelmir Lucena Veiga da Silva, Medical School of Olinda

Professor of Functional Pratices Laboratory;

Coordenator of Study Group on Interactive and Complementary Practices.

Fabiana de Andrade Cavalcante, Federal University of Paraíba

Professor of Patology and Physiology Departament/UFPB

Tania Maria Sarmento da Silva, Rural Federal University of Pernambuco

Professor of Chemical Departament

Alessandro Elery Ramos, Santa Isabel Hospital

Dental Surgeon

Thárcia Kiara Beserra Oliveira, Medical School of Olinda

Professor of the Surgical Skills Laboratory

Schirley Cristina Almeida Pereira, Medical School of Olinda

Teacher of Academy-Community Service Integration

Bagnólia Araújo da Silva, Federal University of Paraíba

Professor of Pharmaceutical Sciences Departament

References

Ajay, M., Gilani, A.H. & Mustafaa, M. R. (2003) Effects of flavonoids on vascular smooth muscle of the isolated rat thoracic aorta. Life Science, 74(5), 603-612.

Bhattacharyya, J., Basilio, I. J. L. D., Morais, L. C. S. L., Agra, M. F. & Majetich G. (2009). Alkaloids of the root-bark of Solanum paludosum Moric. Biochemical Systematics and Ecology, 37, 228–229.

Catterall, W. A., Lenaeus, M. J. & Gamal El-Din, T. M. (2020). Structure and Pharmacology of Voltage-Gated Sodium and Calcium Channels. Annual Review of Pharmacology and Toxicology, 60, 133-154.

De Rojas, V. R. S., Somoza, B., Ortega, T., Villar, A. M. & Tejerina, R. (1999). Vasodilatory effect in rat aorta of eriodictyol obtained from Satureja obovata. Planta Medica, 65(3), 234-238.

Furchgott, R. F. & Zawadki, J. V. (1980). The obligatory role of endothelium cells in the relaxation of arterial smooth muscle by acetylcholine. Nature, 288(5789), 373-376.

Fusi, F., Spiga, O., Trezza, A., Sgaragli, G. & Saponara S. (2017). The surge of flavonoids as novel, fine regulators of cardiovascular Cav channels. European Journal of Pharmacology, 796, 158-174.

GBD 2016 Causes of Death Collaborators. Global, regional, and national age-sex specific mortality for 264 causes of death, 1980- 2016: a systematic analysis for the Global Burden of Disease Study. (2017). Lancet, 390(10100), 1151-210.

Ghosh, D., Syed, A. U., Prada, M. P., Nystoriak, M. A., Santana, L. F., Nieves-Cintrón M. & Navedo, M. F. (2017). Calcium Channels in Vascular Smooth Muscle. Advances in Pharmacology, 78, 49-87

.

Gurney, A. M. (1994). Mechanisms of drug-induced vasodilation. Journal of Pharmacy and Pharmacology, 46(4), 242-251.

Kochegarov, A. A. (2003). Pharmacological modulators of voltage-gated calcium channels and their therapeutical application. Cell Calcium, 33(3), 145-162.

Lin, C. N., Kuo, S. H., Chung, M. I., Ko, F. N. & Teng, C. M. (1997). A new flavone c-glycoside and antiplatelet and vasorelaxing flavones from Gentiana arisanensis. Journal of Natural Products, 60(8), 851-853.

Migkos, T., Pourová, J., Vopršalová, M., Auger, C., Schini-Kerth, V. & Mladěnka, P. (2020). Biochanin A, the Most Potent of 16 Isoflavones, Induces Relaxation of the Coronary Artery Through the Calcium Channel and cGMP-dependent Pathway. Planta Medica, 86(10), 708-716.

Monteiro, F. S., Silva, A. C. L., Martins, I. R. R., Correia, A. C. C., Basílio, I. J. L. D., Agra, M. F., Bhattacharyya, J. & Silva, B. A. (2012). Vasorelaxant action of the total alkaloid fraction obtained from Solanum paludosum Moric. (Solanaceae) involves NO/cGMP/PKG pathway and potassium channels. Journal of Ethnopharmacology, 141, 895-900.

Oliveira, T. K. B., Silva-Júnior, P. R. & Andrade-Filho, J. A. (2019). Modelos experimentais: pesquisa com animais de laboratório [recurso eletrônico]. Aracaju: IFS.

Penso, J., Cordeiro, K. C. F. A., Cunha, C.R.M., Castro, P.F.S., Martins, D. R., Lião, L. M., Rocha, M. L. & Oliveira, V. (2014). Vasorelaxantactivityof7-β-O-glycosides biosynthesized from flavonoids. European Journal of Pharmacology, 733, 75-80.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J. & Shitsuka, R. (2018). Metodologia da pesquisa científica [recurso eletrônico]. Santa Maria, RS: UFSM, NTE.

Riet-Correa, F., Pfister, J., Schild, A. L. & Wierenga, T. (2011). Poisoning by Plants, Mycotoxins and Related Toxins. Joao Pessoa: CAB Internacional.

Sakata K & Karaki H. (1991). Effects of a novel smooth muscle relaxant, KT-362, on contraction and cytosolic Ca2+ level in the rat aorta. British Journal of Pharmacology, 102(1), 174-178.

Schmidt, M. I., Duncan, B. B., Azevedo-Silva, G., Menezes, A. M., Monteiro, C. A., Barreto, S. M. et al. (2011). Chronic non-communicable diseases in Brazil: burden and current challenges. Lancet, 377, 1949-1961.

Schramm, M., Thomas, G., Towart, R. & Franckowiak, G. (1983). Novel dihydropyridines with positive inotropic action through activation of Ca2+ channels. Nature, 303(5917), 535-537.

Silva, T. M. S., Batista, M. M., Camara, C. A. & Agra, M. F. (2005). Molluscicidal activity of some Brazilian Solanum spp. (Solanaceae) against Biomphalaria glabrata. Annals of Tropical Medicine and Parasitology, 99(4), 1-7.

Silva, T. M. S., Braz-Filho, R., Carvalho, M. G., Agra, M. F. (2002). Flavonoids and alkamide from Solanum paludosum Moric. Biochemical Systematics and Ecology, 30, 479-481.

Siqueira, S., Falcão-Silva, V. S., Agra, M. F., Dariva, C., Siqueira-Júnior, J. P. & Fonseca, M. J. V. (2011). Biological activities of Solanum paludosum Moric. extracts obtained by maceration and supercritical fluid extraction. The Journal of Supercritical Fluids, 58, 391–397.

Tew, W. Y., Tan, C. S., Asmawi, M. Z. & Yam, M. F. (2020). Underlying mechanism of vasorelaxant effect exerted by 3,5,7,2′,4′-pentahydroxyflavone in rats aortic ring. European Journal of Pharmacology, 880, 173123.

Vuorela, H., Vuorela, P., Törnquist, K. & Alaranta, S. (1997). Calcium channel blocking activity: screening methods for plant derived compounds. Phytomedicine, 4(2), 167-180.

Wang, H. P., Lu, J. F., Zhang, G. L., Li, X. Y., Peng H. Y., Lu, Y., Zhao, L., Ye, Z. G., Bruce, I. C., Xia, Q. & Qian, L. B. (2014). Endothelium-dependent and -independent vasorelaxant actions and mechanisms induced bytotal flavonoids of Elsholtzia splendens in rat aortas. Environmental Toxicology and Pharmacology, 38(2), 453-9.

Zago, P. M. J. J. ., Meotti, F. L. ., Iukava, L. K. ., Coradette, C. D. D. S. ., Oterio , J., Zardeto-Sabec, G. ., & Boleta-Ceranto, D. D. C. F. (2020). Percepção dos pacientes de um consultório de cardiologia acerca da utilização de plantas medicinais no tratamento da hipertensão arterial. Research, Society and Development, 9(11), e78791110312.

Downloads

Published

17/01/2021

How to Cite

SILVA, J. L. V. da; CAVALCANTE, F. de A.; SILVA, T. M. S. da; RAMOS, A. E.; OLIVEIRA, T. K. B.; PEREIRA, S. C. A.; SILVA, B. A. da . Vasorelaxation endothelium-independent of the ethyl acetate phase from aerial parts of Solanum paludosum Moric. envolves channels-calcium L-type blockade. Research, Society and Development, [S. l.], v. 10, n. 1, p. e31710111845, 2021. DOI: 10.33448/rsd-v10i1.11845. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/11845. Acesso em: 28 feb. 2021.

Issue

Section

Health Sciences