Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivars
DOI:
https://doi.org/10.33448/rsd-v10i2.12554Keywords:
Agricultural biothecnology; Criollo; Fuerte; Fluorescense microscopy; Hygromycin B; Rootstock.Abstract
Avocado (Persea americana) is a fruit crop of economic importance in Ecuador. Currently, a low incidence of Phytophthora cinnamomi has been reported, however, there are other soil pathogens that can affect this crop, even at the initial stages of plant multiplication (nursery), for this reason the use of rootstocks that tolerate these biotic adversities is recommended. In this research, the fungus Fusarium oxysporum was isolated from roots of nursery seedlings with symptoms of necrosis. In addition, an isolate of this pathogenic fungus modified with a strain of Agrobacterium tumefaciens was used to determine the infection of F. oxysporum in the roots of the Fuerte (commercial) and Criollo (local) cultivars. The results allowed to infer that the cultivar Criollo presented a greater tolerance to F. oxysporum than the cultivar Fuerte, which corroborates its use as a rootstock for commercial avocado varieties. Furthermore, to our knowledge, this is the first report of F. oxysporum affecting avocado nursery seedlings in Ecuador.
References
Álvarez, S. P., Quezada, G. Á. & Arbelo, O. C. (2015). El aguacatero (Persea americana MIill). Cultivos Tropicales, 36(2), 111–123.
Barnett, H. L. & Hunter, B. B. (1998). Illustrated genera of imperfect fungi. APS Press.
Bergkessel, M. & Guthrie, C. (2013). Colony PCR. Methods in enzymology, 529: 299-309.
Bruna, A. (1991). Marchitez y pudrición de corona y raíces de espárrago (Asparagus officinalis L.) causado por Fusarium oxysporum Schlechtf. sp. asparagi Cohen. Agricultura Técnica, 51, 52–54.
Bundock, P., den Dulk-Ras, A., Beijersbergen, A. & Hooykaas, P. J. J. (1995). Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. The EMBO Journal, 14(13), 3206-3214.
Caasi, O. C., Walker, N. R., Marek, S. M., Enis, J. N. & Mitchell, T. K. (2010). Infection and colonization of turf-type bermudagrass by Ophiosphaerella herpotricha expressing green or red fluorescent proteins. Phytopathology, 100(5), 415–423.
Cañas-Gutiérrez, G. P., Galindo-López, L. F., Arango-Isaza, R. & Saldamando-Benjumea, C. I. (2015). Diversidad genética de cultivares de aguacate (Persea americana) en Antioquia, Colombia. Agronomía Mesoamericana, 26(1), 129-143.
Corona-Jácome, E. C., Galindo-Tovar, M. E., Lee-Espinosa, H. E. & Landero-Torres, I. (2016). Diversidad genética del aguacate (Persea americana Mill.) en cuatro zonas de su área de dispersión natural. Agroproductividad, 9(6), 80-86.
Cepeda, I. D. B. & Giraldo, A. C. (2011). Concentración mínima inhibitoria de higromicina B en callos embriogénicos de arroz (Oryza sativa L.). Revista Colombiana de Biotecnología, 13(2), 193–198.
Cruz-Martín, M., Acosta-Suárez, M., Rodríguez, E. A., Leiva, M. & Capó, Y. A. (2001). Determinación de la mínima concentración inhibitoria (MCI) de Higromicina B para su utilización como agente de selección en la transformación de Mycosphaerella fijiensis. Biotecnología Vegetal, 1(2), 121-123.
De Oliveira, C. V. S., Gasparotto, L., da SILVA, G. F., Sousa, N. R. & RODRIGUES, S. (2012). O uso de higromicina B, geneticina (G418) e nourseotricina como agentes seletivos visando transformação genética de Fusarium solani f. sp. piperis. Manaus: Embrapa.
Dunn, A. R., Fry, B. A., Lee, T. Y., Conley, K. D., Balaji, V., Fry, W. E., McLeod, A. & Smart, C. D. (2013). Transformation of Phytophthora capsici with genes for green and red fluorescent protein for use in visualizing plant-pathogen interactions. Australasian Plant Pathology, 42(5), 583–593.
Edgar, R. C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32(5), 1792-1797.
Agronomía Colombiana, 38(1), 85-100.
Flores, F. J., Marek, S. M., Anderson, J. A., Mitchell, T. K. & Walker, N. R. (2015). Infection and Colonization of Several Bermudagrasses by Ophiosphaerella korrae. Phytopathology, 105(5), 656-661.
Granada, D., López-Lujan, L., Ramirez-Restrepo, S., Morales, J., Peláez-Jaramillo, C., Andrade, G. & Bedoya-
Pérez, J. C. (2020). Bacterial extracts and bioformulates as a promising control of fruit body rot and root rot in avocado cv. Hass. Journal of Integrative Agriculture, 19(3), 748–758.
Hooykaas, P. J. J., Roobol, C. & Schilperoort, R. A. (1979). Regulation of the transfer of Ti-plasmids of Agrobacterium tumefaciens. Journal of General Microbiology, 110, 99-109.
Kado, C., Heskett, M. & Langley, R. (1972). Studies on Agrobacterium tumefaciens: characterization of strains ID135 and B6, and analysis of the bacterial chromosome, transfer RNA and ribosomes for tumor-inducing ability. Physiological Plant Pathology, 2(1), 47-52.
Molano, P. J. T. (2007). Enfermedades del aguacate. Revista Politécnica, 3(4), 51–70.
Mullins, E. D., Chen, X., Romaine, P., Raina, R., Geiser, D. M. & Kang, S. (2001). Agrobacterium-mediated transformation of Fusarium oxysporum: an efficient tool for insertional mutagenesis and gene transfer. Phytopathology, 91(2), 173–180.
Olalde, G., Raya, Y., Apáez, P., Vargas, M., Pedraza, M., Raymundo, T., Valenzuela, R. & Lara, M. (2020). Characterization of Fusarium spp., a phytopathogen of avocado (Persea americana Miller var. drymifolia (Schltdl. and Cham.) in Michoacán, México. Revista de La Facultad de Ciencias Agrarias. 52(2): 301-316.
Ramírez, J., Catañeda, D. & Morales, J. (2014). Estudios etiológicos de la marchitez del aguacate en Antioquia-Colombia. Revista Ceres, 61(1), 50–61.
Ramírez, J. & Morales, J. (2020). Development and validation of severity scales of avocado wilt complex caused by Phytophthora cinnamomi, Verticillium dahliae and hypoxiaanoxia disorder and their physiological responses in avocado plants.
Rincón-Hernández, C. A., Sánchez Pérez, J. D. L. & Espinosa-García, F. J. (2011). Caracterización química foliar de los árboles de aguacate criollo (Persea americana var. drymifolia) en los bancos de germoplasma de Michoacán, México. Revista Mexicana de biodiversidad, 82(2), 395-412.
Sánchez-González, E. I., Gutiérrez-Soto, J. G., Olivares-Sáenz, E., Gutiérrez-Díaz, A., Barrientos-Priego, A. F. & Ochoa-Ascencio, S. (2019). Screening progenies of mexican race avocado genotypes for resistance to Phytophthora cinnamomi Rands. HortScience, 54(5), 809–813.
Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E. & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology, 22(12), 1567-1572.
Smith, S. A., Beaulieu, J. M. & Donoghue, M. J. (2009). Mega-phylogeny approach for comparative biology: an alternative to supertree and supermatrix approaches. BMC Evolutionary Biology, 9(1), 37.
Toapanta-Gallegos, D. E., Morillo-Velastegui, L. E. & Viera-Arroyo, W. F. (2017). Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador. Ciencia & Tecnología Agropecuaria, 18(2), 285–294.
Tronsmo, A. M., Munk, L., Djurle, A, Yuen, J. & Collinge, D. B. (2020). What is a Plant Disease?. In A.M. Tronsmo, D.B. Collinge, A. Djurle, L. Munk, J. Yuen & A. Tronsmo (Eds.), Plant Pathology and Plant Diseases (pp. 3-10). London, UK: CABI.
Weising, K., Nybom H., Wolff K. & Meyer, W. (1995). DNA fingerprinting in plants and fungi: principles, methods, and applications. Boca Ratón: CRC Press.
Viera, A., Sotomayor, A. & Viera, W. (2016). Potential of avocado cultivation (persea americana mill) in ecuador as an alternative of commercialization in the local and international market. Revista Científica y Tecnológica UPSE, 3(3), 1-9.
Viera, W., Sotomayor, A., Viteri, P., Ushiña, R. & Cho, K. (septiembre de 2017). Germoplasma local de aguacate (Persea americana Mill.) tipo 'Criollo' para la producción de portainjertos en el Ecuador. En J. Cortéz, V Congreso Latinoamericano del Aguacate. Ciudad Guzmán, México.
White, T. J., Bruns, T., Lee, S. J. & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Cambridge: Academic Press, Inc.
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Copyright (c) 2021 Grace Estefanía Uribe Hurtado ; William Fernando Viera Arroyo; Álvaro Xavier Sampedro Lema; Kang Jin Cho; Alicia Beatriz Villavicencio Pazos; Francisco Javier Flores Flor
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