Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes

Authors

DOI:

https://doi.org/10.33448/rsd-v10i3.13038

Keywords:

Bacterium; Diagnostics; Infection diseases; Impact on reproduction; Zoonosis.

Abstract

The present work aimed to evaluate the macro and microscopic lesions and the distribution of Brucella sp. in the reproductive tract of seropositive female buffaloes to brucellosis. Nineteen genital tracts and eighteen peri-vaginal lymph nodes from previous serum reagent animals to brucella were collected from slaughterhouse, processed and submitted to Fisher's exact test (5%). It was not observed uterine macroscopic lesions from non-pregnant and pregnant until the four month, as well as are infrequent in the peri-vaginal lymph nodes. Microscopical lesions were found as lymphocyte clustering in caruncle in the placentome, necrosis and desquamation of chorionic cells, and in the peri-vaginal lymph nodes may occur oedema, increased neutrophil frequency, lymphocyte depletion, lymphoid hyperplasia, congestion, haemorrhage, and vasculitis. Thus, the IHC displayed great sensitivity to identify the presence of the bacterial antigen in tissues of seropositive animals of this species.

References

Ahmed, Y. F., Sokkar, S. M., Ghazi, Y. A., Amin, A. S., & Masboly, A. A. (2010). Pathological and molecular studies on mammary glands and supramammary lymph node of naturally brucella infected buffalo-cows. J Reprod Infertil, 1 (2), 33-40.

Ahmed, Y. F., Sokkar, S. M., Desouky, M., & Madbouly, A. A. (2012). Pathological studies on buffalo-cows naturally infected with Brucella melitensis. Glob Vet, 9 (6), 663-668. 10.5829/idosi.gv.2012.9.6.66168

Baldi, P. C., & Giambartolomei, G. H. (2013). Pathogenesis and pathobiology of zoonotic brucellosis in humans. Rev Sci Tech, 32 (1), 117-125. 10.20506/rst.32.1.2192

Beytut, E., Şahin, M., Erginsoy, S., & Sözmen, M. (2009). Pathological, immunohistochemical, and bacteriological findings in the mammary glands and supramammary lymph nodes of cows with a history of abortion due to Brucella abortus. Turkish J Vet Anim Sci, 33 (1), 37-43. 10.3906/vet-0705-12

Campos, D. I., Coelho, H. E., Kamimura, R., & Arantes, V. M. (2009). Alterações microscópicas em linfonodos de bovinos sorologicamente positivos para brucelose. Arq Ciênc Vet Zool, 12 (2), 123-127. 10.25110/arqvet.v12i2.2009.2965

Corbel, M. J. (2006). Brucellosis in humans and animals. In: Food and Agriculture Organization of the United Nations, World Health Organization & World Organization for Animal Health. ‎Geneva.

Dey, S. K., Rahman, M. S., Rima, U. K., Hossain, M. Z., Chowdhury, G. A., Pervin, M., Habib, M. A. & Khan, M. A. H. N. A. (2013). Serological and pathological investigation of brucellosis in dairy cows of Mymensingh district, Bangladesh. Bangl J Vet Med, 11 (2), 107-112. 10.3329/bjvm.v11i2.19124

Jang, J. S., Do, S. H., Ki, M. R., Hong, I. H., Park, J. K., Cho, Y. J., Park, S. J., Kim, T. H., Kwak, D. M., & Jeong, K. S. (2010). Characterization of bovine Brucellosis in Korean native cattle by means of immunohistochemistry and proteomics. J Life Sci, 20 (2), 153-160. 10.5352/JLS.2010.20.2.153

Le Flèche, P., Jacques, I., Grayon, M., Dahouk, S. A., Bouchon, P., Denoeud, F., Nöckler, K., Neubauer, H., Guilloteau, L. A., & Vergnaud, G. (2006). Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiol, 6 (9). 10.1186/1471-2180-6-9

Meador, V. P., & Deyoe, B. L. (1989a). Intracellular localization of Brucella abortus in bovine placenta. Vet Pathol, 26 (6), 513-515, 10.1177/030098588902600609

Meador, V. P., Deyoe, B. L., & Cheville, N. F. (1989b). Pathogenesis of Brucella abortus infection of the mammary gland and supramammary lymph node of the goat. Vet Pathol, 26 (5), 357-368. 10.1177/030098588902600501

Meirelles-Bartoli, R. B., & Mathias, L. A. (2009). Uso da reação de fixação de complemento na confirmação de resultados positivos do teste do antígeno acidificado tamponado para o diagnóstico sorológico da brucelose bovina. Ars Veter, 25 (2), 68-71. 10.15361/2175-0106.2009v25n2p068-071

Meirelles-Bartoli, R. B., Cruz, C. A., Moraes, R. S., Oliveira, R. A., Paula, E. M. N., Sousa, D. B., Assis, N. A., & Mathias, L. A. (2020). Comparison of four serological tests for the diagnosis of swine brucellosis. Research, Society and Development, 9(7): 1-13, e493974418.

Mense, M. G., Borschel, R. H., Wilhelmsen, C. L., Pitt, M. L., & Hoover, D. L. (2004). Pathologic changes associated with brucellosis experimentally induced by aerosol exposure in rhesus macaques (Macaca mulatta). Am J Vet Res, 65 (5), 644-652. 10.2460/ajvr.2004.65.644

Orlando, D. R., Costa, R. C., Abreu, R. V. S., Abreu, C. C., Nakagaki, K. Y. R., Wouters, A. T. B., Raymundo, D. L., & Varaschin, M. S. (2014). Caracterização morfológica e imuno-histoquímica de lesões em casos de aborto bovino bacteriano e viral no sul de Minas Gerais. Pesq Vet Bras, 34 (10), 974-980. 10.1590/S0100-736X2014001000009.

Paulin, L. M. S., & Ferreira Neto, J. S. (2008). Brucelose em búfalos. Arq Inst Biol, 75 (3), 389-401.

Pereira, A.S., Shitsuka, D.M., Parreira, F.J., Shitsuka, R. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Pérez, J., Quezada, M., López, J., Casquet, O., Sierra, M. A., & Martín de las Mulas, J. (1998). Immunohistochemical detection of Brucella abortus antigens in tissues from aborted bovine fetuses using a commercially available polyclonal antibody. J Vet Diagn Invest, 10 (1), 17-21. 10.1177/104063879801000104

Pranav, P., Sugat, S., & Satadal, D. (2013). Histological changes in mammalian uterus in brucellosis. Asian J Biom Pharm Sci, 3 (22), 58-61.

Sousa, M. G. S., Salvarani, F. M., Bomjardim, H. A., Fonseca Jr, A. A., Preis, I. S., Brito, M. F., Leite, R. C., & Barbosa, J. D. (2015ª). Infecção transplacentária e intrauterina por Brucella abortus em búfalos (Bubalus bubalis). Pesqui Vet Bras, 35 (11), 882-888. 10.1590/S0100-736X2015001100002

Sousa, M. G. S., Brito, M. F., Ubiali, D. G., Fonseca Jr, A. A., Silva, J. B., Belo Reis, A. S., Oliveira, Carlos, M. C., & Barbosa, J. D. (2015b). Detecção de Brucella abortus em linfonodos de búfalas (Bubalus bubalis) em diferentes fases da gestação. Pesqui Vet Bras, 35 (12), 951-955. 10.1590/S0100-736X2015001200003

Sozmen, M., Erginsoy, S. D., Genc, O., Beytut, E., & Özcan, K. (2004). Immunohistochemical and microbiological detection of Brucella abortus antigens in aborted bovine fetuses. Acta Vet Brno, 73, 465-472. 10.2754/avb200473040465

Wang, X., Wang, Y., Ma, L., Zhang, R., De, Y., Yang, X., Wang, C., & Wu, Q. (2015). Development of an improved competitive ELISA based on a monoclonal antibody against lipopolysaccharide for the detection of bovine brucellosis. BMC Vet Res, 11, 118. 10.1186/s12917-015-0436-3

Xavier, M. N., Paixão, T. A., Poester, F. P., Lage, A. P., & Santos, R. L. (2009). Pathological, immunohistochemical and bacteriological study of tissues and milk of cows and fetuses experimentally infected with Brucella abortus. J Comp Pathol, 140 (2-3), 149-157. 10.1016/j.jcpa.2008.10.004

Xavier, M. N., Paixão, T. A., Hartigh, A. B., Tsolis, R. M., & Santos, R. L. (2010). Pathogenesis of Brucella spp. The Open Vet Sci J, 4, 109-118. 10.2174/1874318801004010109

Downloads

Published

06/03/2021

How to Cite

MANRIQUE AYALA, H. D.; SILVA FILHO, E. .; SOUZA , A. J. S. de .; ROLIM FILHO , S. T. .; GARCIA, O. S.; VALE, W. G.; PEREIRA, W. L. A. Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes. Research, Society and Development, [S. l.], v. 10, n. 3, p. e6210313038, 2021. DOI: 10.33448/rsd-v10i3.13038. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/13038. Acesso em: 25 nov. 2024.

Issue

Section

Agrarian and Biological Sciences