In vitro influence of amazonian substances on the dental bleaching




Dental bleaching with açaí; Food pigments; Amazon substances.


Due to the wide scientific divergence of results among in vitro investigations about the influence of food pigmentation on teeth staining during bleaching, this study aims to evaluate in the laboratory the effectiveness of at-home bleaching using carbamide peroxide (CP), and in-office bleaching using hydrogen peroxide (HP) applied to bovine teeth exposed to açaí and guarana energy drink during the dental whitening process. Extracted bovine incisors were randomly separated into six groups as follows: two control groups (at-home bleaching [HB] and in-office bleaching [OB] and immersed in artificial saliva), two groups following the immersion in açaí (HBa and OBa), and two groups following the immersion in guarana (HBg and OBg). The immersion cycle was conducted three times a day for five minutes. After the initial reading, the color was registered subsequently to the third (and last) week of whitening process. This procedure was carried out using Easyshade spectrophotometer and Vita shade guide. The data were submitted to a two-way ANOVA test (p<0.05). The results showed that after three weeks of bleaching, the whitening rate for the OBa group decreased significantly when compared to the other groups, also those submitted to at-home bleaching. After the third week of the whitening process, it was observed that the groups submitted to at-home bleaching presented higher whitening rates than the ones submitted to in-office bleaching. Therefore, the contact with açaí during whitening period may reduce the in-office bleaching efficacy.


Attin, T., Manolakis, A., Buchalla, W., & Hannig, C. (2003). Influence of tea on intrinsic colour of previously bleached enamel. J Oral Rehabil, 30(5), 488-494.

Araujo, J., Dobrovolski, M., Kozlowski Junior, V. A., & Rezende, M. (2021). Use of Plantago major solution for the control of dental biofilm: case report. Research, Society and Development, v. 10, 29610111491-12.

Azer, S.S., Hague, A. L., & Johnston, W. M. (2010). Effect of pH on tooth discoloration from food colorant in vitro. J Dent, 38(2), 106-109.

Berger, S. B., Coelho, A. S., Oliveira, V. A. P., Cavalli, V., & Giannini, M. (2008). Enamel susceptibility to red wine staining after 35% hydrogen peroxide bleaching. J Appl Oral Sci, 16(3), 201-204.

Bernardon, J., Sartori, N., Ballarin, A., Perdigão, J., Lopes, G., & Baratieri, L. (2010). Clinical performance of vital bleaching techniques. Oper Dent, 35(1), 3-10.

Briso, A. L., Fagundes, T. C., Gallinari, M. O., Moreira, J., de Almeida, L., Rahal, V., Gonçalves, R. S., & Santos, P. D. (2016). An in Situ Study of the Influence of Staining Beverages on Color Alteration of Bleached Teeth. Oper Dent, 41(6), 627-633.

Caneppele, T. M. F., Souza, A. C., Valera, M. C., & Pagani, C. (2009). Influence of tooth soaking in substances with dyes regarding the effectiveness of tooth whitening using 16% carbamide peroxide. Arq. Odontol, 45(4), 171-177.

Carlos, N. R., Pinto, A., Amaral, F. D., França, F., Turssi, C. P., & Basting, R. T. (2019). Influence of Staining Solutions on Color Change and Enamel Surface Properties During At-home and In-office Dental Bleaching: An in-Situ Study. Oper Dent, 10.2341/18-236-C.

Cavalli, V., Silva, B. G. D., Berger, S. B., Marson, F. C., Tabchoury, C. P. M., & Giannini, M. (2019). Decomposition Rate, pH, and Enamel Color Alteration of At-Home and In-Office Bleaching Agents. Braz Dent J, 30(4), 385-396.

Costa e Silva, D., Tiradentes, S. B. S. P., Parente, R. C. P., & Bandeira, M. F. C. L. (2009). Color change using HSB color system of dental resin composites immersed in different common Amazon region beverages. Acta Amaz, 39(4), 961-968.

Liporoni, P. C., Souto, C. M., Pazinatto, R. B., Cesar, I. C., de Rego, M. A., Mathias, P., & Cavalli, V. (2010). Enamel susceptibility to coffee and red wine staining at different intervals elapsed from bleaching: a photoreflectance spectrophotometry analysis. Photomed Laser Surg, 28(2), S105-9.

Molina, G. F., Almeida, C. G. R., Guerra, S. C., Cury, J. A., de Almeida, A. P., Barroso, R. C., & Gerlach, R. F. (2011). Lead deposition in bovine enamel during a pH-cycling regimen simulating the caries process. Caries Res, 45(5),469-74.

Mondelli, R. F. L., Azevedo, J. F. D. G., Francisconi, A. C., Almeida, C. M., & Ishikiriama, S. K. (2012). Comparative clinical study of the effectiveness of different dental bleaching methods - two-year follow-up. J Appl Oral Sci, 20(4), 435-443.

Mori, A. A., Lima, F. F., Benetti, A. R., Terada, R. S., Fujimaki, M., & Pascotto, R.C. (2016). Susceptibility to Coffee Staining during Enamel Remineralization Following the In-Office Bleaching Technique: An in-Situ Assessment. J Esthet Restor Dent, 1, S23-31.

Nakonieczna-Rudnicka, M., Bachanek, T., Madejczyki, M., Grajewskai, I., & Kobyłecka, E. (2015). Teeth whitening versus the influence of extrinsic factors on teeth stains. Przegl Lek, 72(3), 126-30.

Nucci, C., Marchionni, S., Piana, G., Mazzoni, A., & Prati C. (2004). Morphological evaluation of enamel surface after application of two “home” whitening products. Oral Health Prev Dent, 2(3), 221-229.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. UFSM. eam/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Pinto, C. F., Oliveira, R., Cavalli, V., & Giannini, M. (2004). Peroxide bleaching agent effects on enamel surface microhardness, roughness and morphology. Pesqui Odontol Bras, 18(4), 306-311.

Rezende, M., Loguercio, A.D., Reis, A., & Kossatz, S. (2013). Clinical effects of exposure to coffee during at-home vital bleaching. Oper Dent, 38(6), 229-236.

Ribeiro, J.C. (2010). Avaliação do potencial antimutagênico da polpa de açaí (Euterpe oleracea Mart) e do óleo de buriti (Mauritia flexuosa) in vivo. Tese de Doutorado da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, São Paulo.

Rodrigues, J. L., Rocha, P. S., Pardim, S. L. S., Machado, A. C. V., Faria-e-Silva, A. L., & Seraidarian, P. I. (2018). Association Between In-Office and At-Home Tooth Bleaching: A Single Blind Randomized Clinical Trial. Braz Dent J, 29(2), 133-139.

Samorodnitzki-Naveh, G. R., Geiger, S. B., & Levin, L. (2007). Patients’ satisfaction with dental esthetics. J Am Dent Assoc, 138(6), 805-8.

Spalding, M., Taveira, L. A., & de Assis, G. F. (2003). Scanning electron microscopy study of dental enamel surface exposed to 35% hydrogen peroxide: alone, with saliva, and with 10% carbamide peroxide. Journal of Esthetic and Restorative Dentistry, 15(3), 154-164.

Sulieman, M. A. (2008). An overview of tooth-bleaching techniques: chemistry, safety and efficacy. Periodontol 2000(48), 148-169.

Zhao, X., Zanetti, F., Wang, L., Pan, J., Majeed, S., Malmstrom, H., Peitsch, M. C., Hoeng, J., Ren, Y. (2019). Effects of different discoloration challenges and whitening treatments on dental hard tissues and composite resin restorations. J Dent.




How to Cite

TAPETY, C. M. C. .; SILVESTRE, F. A. .; MOTA, M. N. G. .; VILAROUCA, L. M. C. .; CARNEIRO, Y. K. P. .; MOREIRA, M. M.; LOGUERCIO, A. D.; FEITOSA, V. P. . In vitro influence of amazonian substances on the dental bleaching. Research, Society and Development, [S. l.], v. 10, n. 3, p. e14010313133, 2021. DOI: 10.33448/rsd-v10i3.13133. Disponível em: Acesso em: 13 may. 2021.



Health Sciences