In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin




Embryos; IGF-I; IGF-II; IVP; Nelore; rbST.


The present work aimed of studying the influence of recombinant bovine somatotropin (rbST) on quantity and quality of in vivo aspirated oocytes, embryo production and gene expression of insulin-like growth factors (IGFs) types I and II in in vitro produced embryos from bovine females of Nelore breed. Five cows were treated with two administrations of 2mL of saline solution as placebo, with an interval of 14 days between them, being the first administration held 19 and the second 5 days before follicular aspirations; within 30 days after these aspirations, in a crossover design, the same protocol was used in these five animals being, in this case, the saline solution replaced by 500mg of rbST in order to perform comparisons between treatments. From retrieved oocytes, in vitro production (IVP) was performed, being all stages involving this process evaluated with subsequent achievement of polymerase chain reactions in real time (qPCRs) for gene expression of IGF-I and IGF-II in produced blastocysts. It was observed that the rbST, although having a numerical increase, did not show significant influence, on the parameters studied, on the quantity and quality of in vivo aspirated oocytes, embryo production and gene expression of IGF-I and IGF-II in in vitro produced embryos from bovine females of Nelore breed.

Author Biography

Emanuel Felipe de Oliveira Filho, Universidade Federal Rural de Pernambuco

Graduated in Veterinary Medicine from UFRPE (2013), Graduated in Agricultural Sciences (2015) by UFRPE. Master in Veterinary Science in the area of nutritional, metabolic diseases and intoxications of ruminants by UFRPE (2016). Doctor in Veterinary Medicine from UFRPE and the University of Santiago de Compostela-Campus Lugo (Spain). PhD student in USC. He acts in the laboratory of Nutritional, Metabolic Diseases and Poisoning of Ruminants; Heavy metals in ruminants and wild animal.


ABIEC. (2020). Associação Brasileira das Indústrias Exportadoras de Carnes. Rebanho Bovino Brasileiro. <>.

Bertolini, M., Beam, S. W., Shim, H., Bertolini, L. R., Moyer, A. L., Famula, T. R., & Anderson, G. B. (2002). Growth, development, and gene expression by in vivo- and in vitro-produced day 7 and 16 bovine embryos. Molecular Reproduction and Development. 63(3), 318-328.

Buratini Jr., J. (2006). Foliculogênese em bovinos; In: 2º Simpósio Internacional de Reprodução Animal Aplicada, 2, 2006, Londrina – PR. Anais... Londrina – PR, 55-62.

IBGE. (2019). Instituto Brasileiro de Geografia e Estatística. Banco de Dados. <>.

Kozicki, L. E., Segui, M. S., Fantini Filho, J. C., Prado, F. R. A., Matté, F., Glaser Jr., P., & Weiss, R. R. (2005). A somatotropina bovina (bST) e sua relação com o recrutamento folicular ovariano durante o ciclo estral de vacas. Archives of Veterinary Science, 10(1), 35-44.

Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT Method. Methods, 25(4), 402-408.

Lonergan, P., Rizos, D., Gutierrez-Adan, A., Moreira, P. M., Pintado, B., & Boland, M. P. (2003). Temporal divergence in the pattern of messenger RNA expression in bovine embryos cultured from the zygote to blastocyst stage in vitro or in vivo. Biology of Reproduction. 69, 1424-1431.

Lucy, M. C. (2000). Regulation of follicular growth by somatotropin and insulin-like growth factors in cattle. Journal of Dairy Science, 83, 1635-1647.

McHughes, C. E., Springer, G. K., Spate, L. D., Li, R., Woods, R., Green, M. P., Korte, S. W., Murphy, C. N., Green, J. A., & Prather, R. S. (2009). Identification and quantification of differentially represented transcripts in in vitro and in vivo derived preimplantation bovine embryos. Molecular Reproduction and Development, 76, 48-60.

Moreira, F., Badingal, L., Burnley, C., & Thatcher, W. W. (2002). Bovine somatotropin increases embryonic development in superovulated cows and improves post-transfer pregnancy rates when given to lactaning recipient cows. Theriogenology, 57, 1371-1387.

Neves, E. F., Ramos, A. F., & Marques Júnior, A. P. (2005). Pré-tratamento com somatotropina bovina (rbST) na superovulação de doadoras da raça Holandesa. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 57, 205-209.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. UFSM. <>.

Ramos, A. A., Ferreira, A. M., Sá, W. F., Viana, J. H. M., Camargo, L. S. A., Polisseni, J., & Henry, M. (2007). Efeito da somatotropina na população folicular, recuperação de oócitos e produção in vitro de embriões em vacas Gir. Revista Brasileira de Zootecnia, 36(2), 380-386.

Ravedutti, G. C., & Vechiato, T. A. F. (2010). Transferência de embriões: aspectos econômicos. Revista Ruminantes, Nugon, 2(13).

Riley, J. K. & Moley, K. H. (2006). Glucose utilization and the PI3-K pathway: mechanisms for cell survival in preimplantation embryos. Reproduction, 131, 823-835.

Roth, Z., Arav, A., Braw-Tal, R., Bor, A., & Wolfenson, A. (2002). Effect of treatment with follicle-stimulating hormone or bovine somatotropin on the quality of oocytes aspirated in the autumn from previously heat-stressed cows. Journal of Dairy Science, 85(6), 1398-1405.

Stringfellow, D. A., & Seidel, S. M. (1998). Manual da Sociedade Internacional de Transferência de Embriões. IETS. 3.

Tripp, M. W., Ju, J. C., Hoagland, T. A., Riesen, J. W., Yang, X., & Zinn, S. A. (2000). Influence of somatotropin and nutrition on bovine oocyte retrievel and in vitro development. Theriogenology, 53(8), 1581-1590.



How to Cite

ANJOS, R. S. dos .; TAVARES , K. C. S. .; MARTINS , L. T. .; AGUIAR, L. H. de . .; BERTOLINI , M. .; OLIVEIRA FILHO, E. F. de .; CARNEIRO, G. F. . In vitro production and gene expression of IGF-I and IGF-II in embryos from Nelore females submitted to administrations of recombinant bovine somatotropin. Research, Society and Development, [S. l.], v. 10, n. 3, p. e27010313263, 2021. DOI: 10.33448/rsd-v10i3.13263. Disponível em: Acesso em: 18 apr. 2021.



Agrarian and Biological Sciences