Gene expression in implant surgery patients: a description of bone and inflammation markers

Authors

DOI:

https://doi.org/10.33448/rsd-v10i3.13650

Keywords:

Ex-smoker; Genes; Hypertension; Osseointegration; Smoking.

Abstract

This research describes the gene expression of molecules related to inflammation and bone metabolism in all patients submitted to dental implant surgery attended at a Brazilian institution. A convenience sample was collected over 2 years. The bone samples were processed for extraction of total RNA and reverse transcription of cDNA. Relative quantification by real-time polymerase chain reaction of target genes was performed. The sample was characterized demographically and according heath conditions. The patients were grouped. The control group was composed of 17 healthy patients, 6 patients were included in the antihypertensive therapy group, 3 were smokers, another 3 were smokers and were under antihypertensive treatment, and 3 were ex-smokers (smoked for 10 years but had quit for at least 5 years). The ex-smoker group presented the highest values, and the Hypertensive group presented the lowest values of Macrophage colony-stimulating factor, Osteonectin, Bone Morphogenetic Protein II, and Integrin. All smokers demonstrated expression values comparable to the Control group. The highest expression values of Osteopontin, RUNX, and Alkaline Phosphatase were observed in the Hypertensive and the Smoker group, while the lowest values were found in the Hypertensive group. Smokers, Ex-smokers, and the Control group demonstrated similar values. As conclusion, gene downregulation was observed in patients under anti-hypertensive therapy. Smoke seems to reduce expression of genes involve in bone formation and increase genes responsible for resorptive activity. This pattern of genic expression may be involved in higher implant loss, observed in smokers. Stop smoke seems to return expression of some of these genes.

Author Biographies

Alessandra Manchini Cardoso Tarallo, São Paulo State University

  •  

Luana Marotta Reis de Vasconcellos, São Paulo State University

Department of Bioscience and Oral Diagnosis

References

Aydoğan, B., Erarslan, E., Ünlütürk, U., & Güllü, S. (2019). Effects of telmisartan and losartan treatments on bone turnover markers in patients with newly diagnosed stage I hypertension. JRAAS - Journal of the Renin-Angiotensin-Aldosterone System, 20(3). https://doi.org/10.1177/1470320319862741

Becker, S. T., Beck-Broichsitter, B. E., Rossmann, C. M., Behrens, E., Jochens, A., & Wiltfang, J. (2016). Long-term Survival of Straumann Dental Implants with TPS Surfaces: A Retrospective Study with a Follow-up of 12 to 23 Years. Clinical Implant Dentistry and Related Research, 18(3), 480–488. https://doi.org/10.1111/cid.12334

Broker, R. de C., Doetzer, A. D., de Souza, C. M., Alvim-Pereira, F., Alvim-Pereira, C. C., & Trevilatto, P. C. (2018). Clinical aspects and polymorphisms in the LTA, TNFA, LTB genes and association with dental implant loss. Clinical Implant Dentistry and Related Research, 20(6), 954–961. https://doi.org/10.1111/cid.12677

Campos, Maria I.G., Santos, M. C. L. G., Trevilatto, P. C., Scarel-Caminaga, R. M., Bezerra, F. J. B., & Line, S. R. P. (2005). Evaluation of the relationship between interleukin-I gene cluster polymorphisms and early implant failure in non-smoking patients. Clinical Oral Implants Research, 16(2), 194–201. https://doi.org/10.1111/j.1600-0501.2004.01091.x

Campos, Maria Isabela Guimarães, Godoy Dos Santos, M. C. L., Trevilatto, P. C., Scarel-Caminaga, R. M., Bezerra, F. J., & Line, S. R. P. (2005). Interleukin-2 and interleukin-6 gene promoter polymorphisms, and early failure of dental implants. Implant Dentistry, 14(4), 391–398. https://doi.org/10.1097/01.id.0000188470.54417.98

Chambrone, L., Preshaw, P. M., Ferreira, J. D., Rodrigues, J. A., Cassoni, A., & Shibli, J. A. (2014). Effects of tobacco smoking on the survival rate of dental implants placed in areas of maxillary sinus floor augmentation: A systematic review. Clinical Oral Implants Research, 25(4), 408–416. https://doi.org/10.1111/clr.12186

Corboz, V. A., Cecchini, M. G., Felix, R., Fleisch, H., van der Pluijm, G., & Löwik, C. W. (1992). Effect of macrophage colony-stimulating factor on in vitro osteoclast generation and bone resorption. Endocrinology, 130(1), 437–442. https://doi.org/10.1210/endo.130.1.1727717

Costa-Junior, F. R., Alvim-Pereira, C. C., Alvim-Pereira, F., Trevilatto, P. C., de Souza, A. P., & Santos, M. C. L. G. (2013). Influence of MMP-8 promoter polymorphism in early osseointegrated implant failure. Clinical Oral Investigations, 17(1), 311–316. https://doi.org/10.1007/s00784-012-0699-z

Dermit, M., Dodel, M., & Mardakheh, F. K. (2017). Methods for monitoring and measurement of protein translation in time and space. Molecular BioSystems, 13, 2477–2488. https://doi.org/10.1039/c7mb00476a

Dirschnabel, A. J., Alvim-Pereira, F., Alvim-Pereira, C. C., Bernardino, J. F., Rosa, E. A. R., & Trevilatto, P. C. (2011). Analysis of the association of IL1B(C-511T) polymorphism with dental implant loss and the clusterization phenomenon. Clinical Oral Implants Research, 22(11), 1235–1241. https://doi.org/10.1111/j.1600-0501.2010.02080.x

do Prado, R. F., Rabêlo, S. B., de Andrade, D. P., Nascimento, R. D., Henriques, V. A. R., Carvalho, Y. R., & de Vasconcellos, L. M. R. (2015). Porous titanium and Ti–35Nb alloy: effects on gene expression of osteoblastic cells derived from human alveolar bone. Journal of Materials Science: Materials in Medicine, 26(11). https://doi.org/10.1007/s10856-015-5594-0

Dole, N. S., Mazur, C. M., Acevedo, C., Lopez, J. P., Monteiro, D. A., Fowler, T. W., & Alliston, T. (2017). Osteocyte-Intrinsic TGF-β Signaling Regulates Bone Quality through Perilacunar/Canalicular Remodeling. Cell Reports, 21(9), 2585–2596. https://doi.org/10.1016/j.celrep.2017.10.115

Gagliardi, C. F., de VasConCellos, L. M. R., Cairo, C. A. A., Rabelo, S. B., & Do Prado, R. F. (2018). Expression of BMP ii by human osteoblasts cultivated on dense or porous titanium. Brazilian Dental Science, 21(3), 275–281. https://doi.org/10.14295/bds.2018.v21i3.1586

Gagné, T., Omorou, A. Y., Kivits, J., Alla, F., & Minary, L. (2018). Socioeconomic profile and smoking among adolescents in vocational training. Revue d’Epidemiologie et de Sante Publique, 66(6), 375–383. https://doi.org/10.1016/j.respe.2018.09.005

Helfrich, M. H., Nesbitt, S. A., Lakkakorpi, P. T., Barnes, M. J., Bodary, S. C., Shankar, G., & Horton, M. A. (1996). β1 integrins and osteoclast function: Involvement in collagen recognition and bone resorption. Bone, 19(4), 317–328. https://doi.org/10.1016/S8756-3282(96)00223-2

Kim, D.-J., Kim, S.-K., Cha, J.-K., Lee, J.-S., & Kim, C.-S. (2019). Clinical Factors and Cellular Responses of In Situ Human Alveolar Bone-Derived Mesenchymal Stromal Cells Associated With Early Periimplant Marginal Bone Loss: A Prospective Cohort Pilot Study. Implant Dentistry, 28(5), 421–429. https://doi.org/10.1097/ID.0000000000000904

Livak, K. J., & Schmittgen, T. D. (2001). Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods, 25(4), 402–408. https://doi.org/10.1006/meth.2001.1262

Maheaswari, R., Kshirsagar, J. T., & Lavanya, N. (2016). Polymerase chain reaction: A molecular diagnostic tool in periodontology. Journal of Indian Society of Periodontology, Vol. 20, pp. 128–135. https://doi.org/10.4103/0972-124X.176391

Montes, C. C., Alvim-Pereira, F., de Castilhos, B. B., Sakurai, M. L. L., Olandoski, M., & Trevilatto, P. C. (2009). Analysis of the association of IL1B (C+3954T) and IL1RN (intron 2) polymorphisms with dental implant loss in a Brazilian population. Clinical Oral Implants Research, 20(2), 208–217. https://doi.org/10.1111/j.1600-0501.2008.01629.x

Montes, C. C., Pereira, F. A., Thomé, G., Alves, E. D. M., Acedo, R. V., de Souza, J. R., & Trevilatto, P. C. (2007). Failing factors associated with osseointegrated dental implant loss. Implant Dentistry, 16(4), 404–412. https://doi.org/10.1097/ID.0b013e31815c8d31

Mulinari-Santos, G., de Souza Batista, F. R., Kirchweger, F., Tangl, S., Gruber, R., & Okamoto, R. (2018). Losartan reverses impaired osseointegration in spontaneously hypertensive rats. Clinical Oral Implants Research, 29(11), 1126–1134. https://doi.org/10.1111/clr.13376

Oliveira, R. M. de, Santos, J. L. F., & Furegato, A. R. F. (2019). Prevalence and smokers’ profile: Comparisons between the psychiatric population and the general population. Revista Latino-Americana de Enfermagem, 27. https://doi.org/10.1590/1518-8345.2976.3149

Opal, S. M., & DePalo, V. A. (2000). Anti-inflammatory cytokines. Chest, 117(4), 1162–1172. https://doi.org/10.1378/chest.117.4.1162

Pereira, T. M., Alvim-Pereira, F., Alvim-Pereira, C. C., Ignácio, S. A., de Souza, C. M., & Trevilatto, P. C. (2019). A complete physical mapping of the VDR gene for dental implant loss: a pilot study. Clinical Oral Implants Research. https://doi.org/10.1111/clr.13529

Pigossi, S. C., Alvim-Pereira, F., Alvim-Pereira, C. C. K., Trevilatto, P. C., & Scarel-Caminaga, R. M. (2014). Association of Interleukin 4 gene polymorphisms with dental implant loss. Implant Dentistry, 23(6), 723–731. https://doi.org/10.1097/ID.0000000000000157

Pigossi, S. C., Alvim-Pereira, F., Montes, C. C., Finoti, L. S., Secolin, R., Trevilatto, P. C., & Scarel-Caminaga, R. M. (2012). Genetic association study between Interleukin 10 gene and dental implant loss. Archives of Oral Biology, 57(9), 1256–1263. https://doi.org/10.1016/j.archoralbio.2012.02.020

Querques, F., Cantilena, B., Cozzolino, C., Esposito, M. T., Passaro, F., Parisi, S., & Pastore, L. (2015). Angiotensin receptor I stimulates osteoprogenitor proliferation through TGFβ-mediated signaling. Journal of Cellular Physiology, 230(7), 1466–1474. https://doi.org/10.1002/jcp.24887

Rodan, G. A., & Noda, M. (1991). Gene expression in osteoblastic cells. Critical Reviews in Eukaryotic Gene Expression, 1(2), 85–98. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/1802105

Saiganesh, S., Saathvika, R., Arumugam, B., Vishal, M., Udhaya, V., Ilangovan, R., & Selvamurugan, N. (2019). TGF-β1-stimulation of matrix metalloproteinase-13 expression by down-regulation of miR-203a-5p in rat osteoblasts. International Journal of Biological Macromolecules, 132, 541–549. https://doi.org/10.1016/j.ijbiomac.2019.04.003

Santos, M. C. L., Campos, M. I. G., Souza, A. P., Trevilatto, P. C., & Line, S. R. P. (n.d.). Analysis of MMP-1 and MMP-9 promoter polymorphisms in early osseointegrated implant failure. The International Journal of Oral & Maxillofacial Implants, 19(1), 38–43. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/14982353

Seki, K., Hasuike, A., Iwano, Y., & Hagiwara, Y. (2020). Influence of antihypertensive medications on the clinical parameters of anodized dental implants: a retrospective cohort study. International Journal of Implant Dentistry, 6(1). https://doi.org/10.1186/s40729-020-00231-9

Shimizu, Y., Nakazato, M., Sekita, T., Kadota, K., Yamasaki, H., Takamura, N., & Maeda, T. (2013). Association between alkaline phosphatase and hypertension in a rural Japanese population: The Nagasaki Islands study. Journal of Physiological Anthropology, 32(1), 10. https://doi.org/10.1186/1880-6805-32-10

Stpień, E., Fedak, D., Klimeczek, P., Wilkosz, T., Banyś, R. P., Starzyk, K., & Pasowicz, M. (2012). Osteoprotegerin, but not osteopontin, as a potential predictor of vascular calcification in normotensive subjects. Hypertension Research, 35(5), 531–538. https://doi.org/10.1038/hr.2011.231

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Published

22/03/2021

How to Cite

PRADO, R. F. do .; TARALLO, A. M. C. .; VASCONCELLOS, L. M. R. de .; SILVA, A. M. .; NOGUEIRA JUNIOR, L. .; SAAVEDRA, G. de S. F. A. .; NISHIOKA, R. S.; BORGES, A. L. S. . Gene expression in implant surgery patients: a description of bone and inflammation markers. Research, Society and Development, [S. l.], v. 10, n. 3, p. e46910313650, 2021. DOI: 10.33448/rsd-v10i3.13650. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/13650. Acesso em: 12 apr. 2021.

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Health Sciences