Essential Oil of Lippia origanoides (Verbenaceae) in cleaning of teats dairy cows

Authors

DOI:

https://doi.org/10.33448/rsd-v10i4.13808

Keywords:

Antiseptic; Pepper rosemary; Bovine mastitis; Pre-immersion.

Abstract

The present study aimed with the objective evaluate the effectiveness of a new product made with essential oil of pepper rosemary under the reduction of the count of microorganisms present in the skin of teats of dairy cows. For the in vivo test, the effectiveness of the new product under negative antisepsis for clinical mastitis (192 teats), was evaluated in comparison with the treatment containing commercial products (192 teats), in a period of 42 days. The teats were monitored clinically daily, with a weekly assessment of the microbiota present on their skin as to a reduction in the count of Staphylococcus aureus, coagulase negative staphylococci (SNC), Echerichia coli and mesophilic aerobes. Both products maintained the integrity of the teats, with the absence of clinical mastitis observed during the experimental period. There was no significant difference in the occurrence of subclinical mastitis and microorganisms on the teats of the two groups under study by Fisher’s test at a significance level of 5%. When companing the log reduction of the count in UFC.mL-1 of the microorganisms for the group treated with conventional product and with experimental product for aerobic mesophiles, E. coli, S. aureus and coagulase negative staphylococci, observed with the cleaning process of the teats, a Statistical difference was observed (p>0,05), in the counting of microorganisms, showing that both effect similar to the control of microorganisms present in the skin of the teats. Thus, the new product containing essential oil of pepper rosemary showed similar efficacy to the convencional product chlorhexidine and iodine for antisepsis of dairy cow teats without promoting local reactions on the skin, and can be used as an alternative product in the handling of milking.

References

Adams, R. B. (2012). Identification of essential oil components by gas chromatogrophy/mass spectrometry. Carol Stream: Allured Publishing Corporation, 804 p.

Aguiar, M. C. S., Silvério, F. O., Pinho, G. P., Lopes, P. S. N. L., Fidêncio, P. H., & Ventura S. J. (2014). Volatile compounds from fruits of Butia capitata at different stages of maturity and storage. Food Research International, 62, 1095-1099. 10.1016/j.foodres.2014.05.039.

Ahvanooei, M. R., Norouzian, M., & Hedayati, M. (2020). Iodine Concentration in Iranian Dairy Milk Products and Its Contribution to the Consumer’s Iodine Intake. Iranian Journal of Veterinary Medicine, 14(2), 159-165. 10.22059/ijvm.2019.280325.1004984.

Ahvanouei, M. R. R., Norouzian, M. A., Hedayati, M., & Vahmani, P. (2021). Effect of potassium iodide supplementation and teat-dipping on iodine status in dairy cows and milk iodine levels. Domestic Animal Endocrinology, 74, 106504. 10.1016/j.domaniend.2020.106504.

Alawneh, J. I., James, A. S., Phillips, N., Fraser, B., Jury, K., Soust, M., & Olchowy, T. (2020). Efficacy of a Lactobacillus-Based Teat Spray on Udder Health in Lactating Dairy Cows. Frontiers in veterinary science, 7, 584436. 10.3389/fvets.2020.584436.

Amaral, L. A., Isa, H., Dias, L. T., Rossi Jr, O. D., & Filho, A. N. (2004). Avaliação da eficiência da desinfecção de teteiras e dos tetos no processo de ordenha mecânica de vacas. Pesquisa Veterinária Brasileira, 24(4), 173-177, 2004. https://www.scielo.br/pdf/pvb/v24n4/a01v24n4.

Apha, American public health association (2001). Compendium of methods for the microbiological examination of foods. (4th ed.) APHA. 25- 35. https://ajph.aphapublications.org/doi/book/10.2105/MBEF.0222.

Barbosa, L. N., Almada, A. F. B., Schmitz Junior, J. A., Vechio, M. A. D., Bezerra, K., Espolador, G. F., Santos, M. C. Dos., Moretto, L. De F., Santos, I. C., Martins, L. De A., &Gonçalves, D. D Bacteriophages’ action against mastitis-causing bactéria. Research, Society and Development, 9,10, e1849108541, 2020. 10.33448/rsd-v9i10.8541.

Baumberger C., Guarin J. F., & Ruegg P. L. (2016). Effect of 2 Different Premilking Teat Sanitation Routines on Reduction of Bacterial Counts on Teat Skin of Cows on Commercial Dairy Farms. Journal of dairy Science, 99(4), 2915–2929. 10.3168/jds.2015-10003.

Bhutto, A. L., Murray, R. D., & Woldehiwet, Z. (2010). Udder shape and teat-end lesions as potential risk factors for high somatic cell counts and intra-mammary infections in dairy cows. The Veterinary Journal, 183(3), 63–67. 10.1016/j.tvjl.2008.08.024.

Braem, G., Vliegher, S., Verbist, B., Piessens, B., Van Coillie, E., De Vuyst, L., & Leroy, F. (2013). Unraveling the microbiota of teat apices of clinically healthy lactating dairy cows, with special emphasis on coagulase-negative staphylococci. Journal of Dairy Science, 96(3), 1499-1510. doi:10.3168/jds.2012-5493.

Cavalcante, D. A., Leite Júnior, B. R. C., Tribst, A. A. L., Cristianini, M., & Coelho, V. R. P. (2013). Uso da água ozonizada na sanitização dos tetos de Bovinos e sua influência na qualidade do leite. Revista do Instituto de Laticínios Candido Tostes, 68(392), 33-39. 10.5935/2238-6416.20130026.

Ceballos-Marquez, A., Hemling, T., Rauch, B. J., Lopez-Benavides, M., & Schukken, Y. H. (2013). Noninferiority trial on the efficacy of premilking teat disinfectant against naturally occurring new intramammary infections using a novel 2-step diagnostic process. Journal of Dairy Science, 96(12), 8081-8092. doi:10.3168/jds.2013-7108.

Clinical and Laboratory Standards Institute (CLSI). (2012). Methods for Dilution Antimicrobial Susceptibility Tests f or Bacteria That Grow Aerobically; Approved Standard—Ninth Edition. CLSI document M07-A9. Wayne, PA: Clinical and Laboratory Standards Institute; 2012a. http://antimicrobianos.com.ar/ATB/wp-content/uploads/2012/11/03-CLSI-M07-A9-2012.pdf.

Coneyworth, L. J., Coulthard, L. C. H. A., Elizabeth H., Bailey, E. H., Young, D. S., Stubberfield, J., Parsons, L., Saunders, N., Watson, E., Homer, E. M., & Welham, S. J. M. (2020). Geographical and seasonal variation in iodine content of cow’s milk in the UK and consequences for the consumer´s supply. Journal of Trace Elements in Medicine and Biology. 59, 126453. 10.1016/j.jtemb.2020.126453.

Costa, L. B., Rajala-Schultz, P. J., Hoet, A., Seo, K. S., Fogt, K., & Moon, B. S. (2014). Genetic relatedness and virulence factors of bovine Staphylococcus aureus isolated from teat skin and milk. Journal Dairy Science, 97(11), 6907-6916. 10.3168/jds.2014-7972.

Enger, B. D., Fox, L. K., Gay, J. M., Johnson, K. A. (2015). Reduction of teat skin mastitis pathogen loads: Differences between strains, dips, and contact times. Journal of Dairy Science, 98(2), 1354-1361. 10.3168/jds.2014-8622.

Fitzpatrick S. R., Garvey M., Flynn J., Jordan K., & Gleeson D. (2019). Are some teat disinfectant formulations more effective against specific bacteria isolated on teat skin than others? Acta Veterinaria Scandinavica, 61, 1–5. 10.1186/s13028-019-0455-3.

Fitzpatrick, S. R., Garvey, M., Flynn, J., O’Brien, B., Gleeson, D. (2021). O efeito dos ingredientes desinfetantes nas bactérias da pele do teto associadas à mastite em rebanhos leiteiros irlandeses. Irish Veterinary Journal, 74, 1 (2021). 10.1186/s13620-020-00179-7.

Klostermann, K., Crispie, F., Flynn, J., Meaney, W., Ross, R. P., & Colin, H. (2009). Efficacy of a teat dip containing the bacteriocin lacticin 3147 to eliminate Gram-positive pathogens associated with bovine mastitis. Journal of Dairy Research, 77(2), 231-238, 2009. 10.1017/S0022029909990239.

Klostermann, K., Crispie, F., Flynn, J., Ross, R. P., Hill, C., & Meaney, W. (2008). Intramammary infusion of a live culture of Lactococcus lactis for treatment of bovine mastitis: comparison with antibiotic treatment in field trials. Journal of Dairy Research, 75(3), 365-373. 10.1017/S0022029908003373.

Langoni, H. (2013). Qualidade do leite: utopia sem um programa sério de monitoramento da ocorrência de mastite bovina. Revista Pesquisa Veterinária Brasileira, 33(5), 620-626. 10.1590/S0100-736X2013000500012.

Mahmmod, Y. S., Klaas, I. C., Svennesen, L., Pedersen, K., & Ingmer, H. (2018). Communications of Staphylococcus aureus and non-aureus Staphylococcus species from bovine intramammary infections and teat apex colonization. Journal Dairy Science, 101(8), 7322–7333. 10.3168/jds.2017-14311.

Marcelo, N. A., Andrade, V. A., Souza, C. N., Mourão, R. P., Mourthe, M. H. F., Silva, L. M. V., Xavier, A. R. E. O., Xavier, M. A. S., Faraco, A. A. G., & Almeida, A. C. (2020). Efficacy of novel antiseptic product containing essential oil of Lippia origanoides to reduce intramammary infections in cows. Veterinary World, 13(11), 2452-2458. 10.14202%2Fvetworld.2020.2452-2458.

Meins, G. A., Neijenhuis, F., Morgan, W. F., Reinemann, D. J., Hillerton, J. E., Baines, J. R., Ohnstad, I., Rasmussen, M. D., Timms, L., Britt, J. S., Farnsworth, R., Cook, N., & Hemlimg, T. (2001). Evaluation of bovine teat condition in commercial dairy herds: 1.Non-infectious factors.In: Second International Symposium on Mastitis and Milk Quality Proceedings, BC, Canada, 347-351.

Ministério da Agricultura Pecuária e Abastecimento (2008). Instrução Normativa n°64, de 18 de dezembro de 2008. Aprova Regulamento Técnico para os Sistemas Orgânicos de Produção Animal e Vegetal. Diário Oficial da Uniãode 19 de Dezembro de 2008, Seção 1, Página 21, Brasília, DF.

Ministério da Agricultura, Pecuária e Abastecimento (2009). Instrução Normativa MAPA nº 26, de 9 de Julho de 2009. Aprova o Regulamento Técnico para a Fabricação, o Controle de Qualidade, a Comercialização e o Emprego de Produtos Antimicrobianos de Uso Veterinário. Diário Oficial da Uniãode 22 de abril de 2004, Seção 1, Página 211, Brasília, DF.

Morton, J. M., Penry, J. F., Malmo, J., & Mein, G. A. (2014). Premilking teat disinfection: Is it worthwhile in pasture-grazed dairy herds?. Journal of Dairy Science, 97 (12), 7525-7537. 10.3168/jds.2014-8185.

Nickerson, S. C. (2001). Choosing the best teat dip for mastitis control and milk quality. In NMC-Milk quality conference proceedings, 43. http://www.nmconline.org/articles/teatdip.htm.

Nickerson, S. C., Saxon, A., Fox, L. K., Hemling, T., Hogan, J. S., Morelli, J., Oliver, S. P. Owens, W. E., Pawlak, M., & Peterson, L. (2004). Recommended protocols for evaluating efficacy of postmilking teat germicides. NMC Annual Meeting Proceedings, 379-399. https://bit.ly/3puxbfN.

Oliveira, S. P. de, Xavier, A. R. E. de O., Souza, C. N. de, Cunha, G. S. P., Melo Júnior, A. F. de, Xavier, M. A. de S., Sanglard, D. A., & Almeida, A. C. de. (2016). Identificação genotípica de Staphylococcus aureus multiresistentes a drogas isolados de animais de produção. Caderno De Ciências Agrárias, 8(3), 45–51. https://periodicos.ufmg.br/index.php/ccaufmg/article/view/2942.

Paduch, J. H., Mohr, E., & Kromekera, V. (2012). The association between teat end hyperkeratosis and teat canal microbial load in lactating dairy cattle. Veterinary Microbiology, 158(3), 353–359. 10.1016/j.vetmic.2012.02.032.

Pereira, A. S., Shitsuka, F. J. Parreira, F. J., & Shitsuka, R. Metodologia da pesquisa científica. UFSM.

Piessens, V., De Vliegher, S., Verbist, B., Braem, G. Van Nuffel, A., De Vuyst, L., Heyndrickx, M., & Van Coillie, E. (2012). Characterization of coagulase-negative staphylococcus species from cows’ milk and environment based on bap, icaa, and meca genes and phenotypic susceptibility to antimicrobials and teat dips. Journal of Dairy Science, 95(12), 7027-7038. 10.3168/jds.2012-5400.

Piessens, V., Van Coillie, E., Verbist, B., Supré, K., Braem, G., Van Nuffel, A., De Vuyst, Heyndrickx, M., & De Vliegher, S. (2011). Distribution of coagulase-negative Staphylococcus species from milk and environment of dairy cows differs between herds. Journal of Dairy Science, 94(6), 2933-2944. 10.3168/jds.2010-3956.

Portaria n° 101, de 17 de agosto de 1993. (1993). Ministério da Agricultura, Pecuária e Abastecimento. Métodos de Análise Microbiológica para Alimentos. Diário Oficial, 11937-11945

Quirk, T., Fox, L. K., Hancock, D. D., Capper, J., Wenz, J., & Park, J. (2012). Intramammary infections and teat canal colonization with coagulase-negative staphylococci after postmilking teat disinfection: Species-specific responses. Journal of Dairy Science, 95(4), 1906–1912. 10.3168/jds.2011-4898.

R Development Core Team. (2013). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0. http://www.R-project.org.

Reyher, K. K., Haine, D., Dohoo, I. R., & Revie, C. W. (2012). Examining the effect of intramammary infections with minor mastitis pathogens on the acquisition of new intramammary infections with major mastitis pathogens—A systematic review and meta-analysis. Journal of Dairy Science, 95(11), 6483–6502. 10.3168/jds.2012-5594.

SAS Institute Inc. SAS® 9.4 System Options: Reference. Cary, NC: SAS Institute Inc, 2013.

Schiavon, D. B. A., Schuch, L. F. D., Oyarzabal, M. E. B., Prestes, L. S., Zani, J. L., & Hartwig, C. A. (2011). Aplicación de plantas medicinales para La antisepsia de pezones de vacas posordeño. Revista Cubana de Plantas Medicinales, 16(6), 253-259. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962011000300005.

Svennesen, L., Mahmmod, Y. S., Skjølstrup, N. K., Mathiasen, L. R., Katholm, J., Pedersen, K., Klaas, I. C., & Nielsen, S. S. (2018). Accuracy of qPCR and bacterial culture for the diagnosis of bovine intramammary infections and teat skin colonisation with Streptococcus agalactiae and Staphylococcus aureus using Bayesian analysis. Preventive Veterinary Medicine, 161, 69–74. 10.1016/j.prevetmed.2018.10.013.

Van der Reijden, O. L., Galetti, V., Herter-Aeberli, I., Zimmermann, M. B., Zeder, C., Krzystek, A., Haldimann, M., Barmaz, A., Kreuzer, M., Berard, J., & Schlegel, P. (2019). Effects of feed iodine concentrations and milk processing on iodine concentrations of cow’s milk and dairy products, andpotential impact oniodine intake in Swiss adults. British Journal of Nutrition, 122(2), 172-185. 10.1017/s0007114519001041.

Van der Reijden, O., Galetti, V., Hulmann, M., Krzystek, A., Haldimann, M., Schlegel, P., Manzocchi, E., Berard, J. J., Kreuzer, M., Zimmermann, M. B., & Herter-Aeberli, I. 2018. The main determinants of iodine in cows’ milk in Switzerland are farm type, season and teat dipping. British Journal of Nutrition, 119(5), 559-569. 10.1017/S0007114517003798.

Verdier-Metz, I., Gagne, G., Bornes, S., Monsallier, F., Veisseire, P., Delbès-Paus, C., & Montel, M.-C. (2011). Cow Teat Skin, a Potential Source of Diverse Microbial Populations for Cheese Production. Applied and Environmental Microbiology, 78(2), 326–333. 10.1128/aem.06229-11.

Yu, J. Yan, R., Xi, X., Huang, W., & Heping, Z. (2017). A Novel Lactobacilli-Based Teat Disinfectant for Improving Bacterial Communities in the Milks of Cow Teats with Subclinical Mastitis.Frontiers in Microbiology, 8, 1782. 10.3389/fmicb.2017.01782.

Zoche-Golob, V., Haverkamp, H., Paduch, J.-H., Klocke, D., Zinke, C., Hoedemaker, M., Heuwieser, W., & Krömker, V. (2015). Longitudinal study of the effects of teat condition on the risk of new intramammary infections in dairy cows. Journal of Dairy Science, 98(2), 910–917. 10.3168/jds.2014-8446.

Published

24/04/2021

How to Cite

ALMEIDA, A. C. de; MOURÃO, R. P. .; MOURTHÉ, M. H. F. .; FARACO, A. A. G. .; FONSECA, F. S. A. .; MARTINS, E. R. .; SILVA, L. M. V. .; SOUZA, C. N. de .; MARCELO, N. A. .; VIEIRA, L. G.; PORTO, R. . R.; LIMA, M. D. de .; LOPES, I. M. G. .; SANTOS, E. M. S. Essential Oil of Lippia origanoides (Verbenaceae) in cleaning of teats dairy cows. Research, Society and Development, [S. l.], v. 10, n. 4, p. e57410413808, 2021. DOI: 10.33448/rsd-v10i4.13808. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/13808. Acesso em: 7 may. 2021.

Issue

Section

Agrarian and Biological Sciences