Interaction between bark extract of Anadenanthera colubrina var. cebil (Griseb.) Altschul with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA)

Authors

DOI:

https://doi.org/10.33448/rsd-v10i6.15469

Keywords:

Fabaceae; Staphylococcus aureus; Angico; Caatinga; MRSA.

Abstract

Anadenanthera colubrina var. cebil (Griseb.) Altschul, a plant often found in areas of the Caatinga in northeastern Brazil, is widely used in unconventional medicine for the treatment of infections and inflammations. Thus, the aim of the present study was to evaluate the antibacterial activity of A. colubrina bark extracts against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates, to investigate if intact and regenerated bark extracts have the same effect against MRSA and to determine the interaction between these extracts and antibiotics. The antibacterial activity was performed by the determination of the minimum inhibitory concentration (MIC) according to the Clinical and Laboratory Standards Institute (CLSI) and the interaction assay was performed by the checkerboard method. A. colubrina extracts showed bacteriostatic activity (MIC = 8-32 mg/L) against MRSA clinical strains and no difference was found in antibacterial activity between intact and regenerated barks, suggesting that even after regeneration, the barks of this species have the same antibacterial activity. Moreover, the in vitro interaction of A. colubrina extracts with ciprofloxacin or erythromycin was additive (FICI = 0.52). Thus, the bark extracts of Anadenanthera colubrina exhibit antibacterial activity and can be used alone or in combination with antibiotics against MRSA clinical isolates.

Author Biographies

Pérola Paloma Silva do Nascimento , Centro Acadêmico de Vitória

Graduada em Ciências Biológicas pela Universidade Federal de Pernambuco (UFPE). Especialista em Ensino de Ciências Biológicas pelo Centro Universitário da Vitória de Santo Antão (UNIVISA). Mestranda pelo Programa de Pós-Graduação em Morfotecnologia pela UFPE.

Rafaela de Siqueira Ferraz-Carvalho, Universidade Federal de Pernambuco

Graduada em Ciências Biológicas pela Universidade Federal de Pernambuco (UFPE). Mestre e doutora pelo Programa de Pós-graduação em Ciências Biológicas da UFPE (PPGCB/UFPE). Pós-doutora pelo programa PNPD CAPES/FACEPE.

Thaís Soares da Silva, Centro Acadêmico de Vitória

Graduada em Ciências Biológicas pela Universidade Federal de Pernambuco (UFPE). Mestre em Educação pela UFPE. Doutoranda no Programa de Pós-Graduação em Ensino da Ciências pela Universidade Federal Rural de Pernambuco (PPGEC/UFRPE).

Thiago Antônio de Sousa Araújo, Universidade Federal de Pernambuco

Graduado em Ciências Biológicas pela Universidade de Pernambuco (UPE). Mestre e Doutor em Ciências Farmacêuticas pela Universidade Federal de Pernambuco (PPGCF/UFPE).

Elba Lúcia Cavalcanti de Amorim, Universidade Federal de Pernambuco

Graduada em Farmácia pela Universidade Federal de Pernambuco (UFPE) e Graduada em Engenharia Química pela Universidade Católica de Pernambuco (UNICAP). Mestre em Ciências Farmacêuticas pela UFPE. Doutorado em Química Orgânica pela Université Pierre et Marie Curie - Paris VI. Professora associada 2 da UFPE.

Marcela Araújo Pereira, Universidade Federal de Pernambuco

Graduada em Biomedicina na Universidade Federal de Pernambuco. Mestre em Ciências Biológicas pela Pós-Graduação de Ciências Biológicas na Universidade Federal de Pernambuco (PPGCB/UFPE). Doutora em Biologia Aplicada à Saúde, pela Universidade Federal de Pernambuco (PPGBAS/UFPE), com doutorado sanduíche (período de 1 ano) na Universidade de Navarra, Espanha.

References

Agra, M. F., Baracho, G. S., Nurit, K., Basílio, I. J. L. D., & Coelho, V. P. M. (2007). Medicinal and poisonous diversity of the flora of “Cariri Paraibano”, Brazil. Journal of Ethnopharmacology, 111(2), 383–395.

Akio Tanaka, J. C., Da Silva, C. C., Dias Filho, B. P., Nakamura, C. V., De Carvalho, J. E., & Foglio, M. A. (2005). Constituintes químicos de Luehea divaricata Mart. (Tiliaceae). Quimica Nova, 28(5), 834–837.

Albano, M., Alves, F. C. B., Andrade, B. F. M. T., Barbosa, L. N., Pereira, A. F. M., de Souza, M. de L. R., Rall, V. L. M., et al. (2016). Antibacterial and anti-staphylococcal enterotoxin activities of phenolic compounds. Innovative Food Science & Emerging Technologies, 38, 83–90. Elsevier.

Araújo, R. M. S. De, Ferreira, R. D. S., Napoleão, T. H., Carneiro-da-Cunha, M. D. G., Coelho, L. C. B. B., Correia, M. T. dos S., Oliva, M. L. V., et al. (2012). Crataeva tapia bark lectin is an affinity adsorbent and insecticidal agent. Plant Science, 183, 20–26.

Araújo, T. A. de S., Alencar, N. L., Amorim, E. L. C. de, & Albuquerque, U. P. de. (2008). A new approach to study medicinal plants with tannins and flavonoids contents from the local knowledge. Journal of Ethnopharmacology, 120(1), 72–80.

Araújo, T. A. de S., Almeida e Castro, V. T. N. de, Solon, L. G. da S., Silva, G. A. da, Almeida, M. G., Costa, J. G. M. da, Amorim, E. L. C. de, et al. (2015). Does rainfall affect the antioxidant capacity and production of phenolic compounds of an important medicinal species? Industrial Crops and Products, 76, 550–556.

Braga, L. C., Leite, A. A. M., Xavier, K. G. S., Takahashi, J. A., Bemquerer, M. P., Chartone-Souza, E., & Nascimento, A. M. A. (2005). Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus. Canadian Journal of Microbiology, 51(7), 541–547.

Cavalcanti, I. M. F., Menezes, T. G. C., Campos, L. A. de A., Ferraz, M. S., Maciel, M. A. V., Caetano, M. N. P., & Santos-Magalhães, N. S. (2018). Interaction study between vancomycin and liposomes containing natural compounds against methicillin-resistant staphylococcus aureus clinical isolates. Brazilian Journal of Pharmaceutical Sciences, 54(2).

Clinical And Laboratory Standards Institute (CLSI). (2020). Performance Standards for Antimicrobial Susceptibility Testing. Clinical And Laboratory Standards Institute (CLSI). (30th ed.). Wayne, PA.

Faria, N. A., Oliveira, D. C., Westh, H., Monnet, D. L., Larsen, A. R., Skov, R., & de Lencastre, H. (2005). Epidemiology of emerging methicillin-resistant Staphylococcus aureus (MRSA) in Denmark: a nationwide study in a country with low prevalence of MRSA infection. Journal of Clinical Microbiology, 43(4), 1836–1842. Am Soc Microbiol.

Figueredo, Fernando G., Ferreira, E. O., Lucena, B. F. F., Torres, C. M. G., Lucetti, D. L., Lucetti, E. C. P., Silva, J. M. F. L., et al. (2013). Modulation of the antibiotic activity by extracts from amburana cearensis A. C. Smith and anadenanthera macrocarpa (Benth.) Brenan. BioMed Research International, 2013.

Figueredo, Fernando Gomes, Ramos, I. T. L., Paz, J. A., Silva, T. M. S., Câmara, C. A., de Morais Oliveira-Tintino, C. D., Tintino, S. R., et al. (2020). Effect of hydroxyamines derived from lapachol and norlachol against Staphylococcus aureus strains carrying the NorA efflux pump. Infection, Genetics and Evolution, 84, 104370. Elsevier B.V. Retrieved from https://doi.org/10.1016/j.meegid.2020.104370

Gibbons, S. (2004). Anti-staphylococcal plant natural products. Natural Product Reports, 21(2), 263–277.

Hiramatsu, K. (2001). Vancomycin-resistant Staphylococcus aureus: A new model of antibiotic resistance. Lancet Infectious Diseases, 1(3), 147–155.

Holetz, F. B., Pessini, G. L., Sanches, N. R., Cortez, A. G., Nakamura, C. V., Prado, B., & Filho, D. (2002). Screening of Some Plants Used in the Brazilian Folk Medicine for the Treatment of Infectious Diseases. Mem Inst Oswaldo Cruz, 97(October), 1027–1031.

Jackson, C., Agboke, A., & Nwoke, V. (2009). In vitro evaluation of antimicrobial activity of combinations of nystatin and Euphorbia hirta leaf extract against Candida albicans by the checkerboard method. Journal of Medicinal Plants Research, 3(9), 666–669.

Jandú, J. J. B., Silva, L. C. N., Silva, M. V, & Correia, M. T. S. (2015). Antimicrobial activity and synergistic effects of an ethyl acetate fraction from methanol extract of Myracrodruon urundeuva bark. J of Medicinal Plants Research, 9, 641–646.

Katalinic, V., Mozina, S. S., Generalic, I., Skroza, D., Ljubenkov, I., & Klancnik, A. (2013). Phenolic profile, antioxidant capacity, and antimicrobial activity of leaf extracts from six vitis vinifera L. Varieties. International Journal of Food Properties, 16(1), 45–60.

Kluczynik, C. E. N., Souza, J. H. de, Palmeira, J. D., Ferreira, S. B., Antunes, R. M. P., Arruda, T. A. de, Morais, M. R., et al. (2010). Perfil de sensibilidade de Salmonella sp. de ambiente aquático a antimicrobianos comerciais e a extratos hidroalcoólicos de plantas medicinais. Rev. bras. anal. clin, 42(2), 141–144.

Lima, A. R. N., Macedo, R. G., Batista, G. G., Câmara, G. B., Lima, R. de F., & Oliveira, T. K. B. de. (2020). Atividade antimicrobiana e anti-inflamatória da Anadenanthera colubrina (Vell.) Brenan. Research, Society and Development, 9(1), e121911770. Retrieved from http://dx.doi.org/10.33448/rsd-v9i1.1770

Lima, R. D. F., Alves, É. P., Rosalen, P. L., Ruiz, A. L. T. G., Teixeira Duarte, M. C., Góes, V. F. F., De Medeiros, A. C. D., et al. (2014). Antimicrobial and antiproliferative potential of anadenanthera colubrina (Vell.) brenan. Evidence-based Complementary and Alternative Medicine, 2014.

Macêdo, L., Fernandes, T., Silveira, L., Mesquita, A., Franchitti, A. A., & Ximenes, E. A. (2013). β-Lapachone activity in synergy with conventional antimicrobials against methicillin resistant Staphylococcus aureus strains. Phytomedicine, 21(1), 25–29. Elsevier GmbH. Retrieved from http://dx.doi.org/10.1016/j.phymed.2013.08.010

Machado, T. B., Pinto, A. V., Pinto, M. C. F. R., Leal, I. C. R., Silva, M. G., Amaral, A. C. F., Kuster, R. M., et al. (2003). In vitro activity of Brazilian medicinal plants, naturally occurring naphthoquinones and their analogues, against methicillin-resistant Staphylococcus aureus. International Journal of Antimicrobial Agents, 21(3), 279–284.

Mairi, A., Touati, A., & Lavigne, J. P. (2020). Methicillin-Resistant Staphylococcus aureus ST80 Clone: A systematic review. Toxins, 12(2), 119. Multidisciplinary Digital Publishing Institute.

Manekeng, H. T., Mbaveng, A. T., Nguenang, G. S., Seukep, J. A., Wamba, B. E. N., Nayim, P., Yinkfu, N. R., et al. (2018). Anti-staphylococcal and antibiotic-potentiating activities of seven Cameroonian edible plants against resistant phenotypes. Investigational Medicinal Chemistry and Pharmacology, 1(1).

Marques, D. N., Almeida, A. S. de, Sousa, A. R. de O., Pereira, R., Andrade, A. L., Chaves, R. P., Carneiro, R. F., et al. (2018). Antibacterial activity of a new lectin isolated from the marine sponge Chondrilla caribensis. International Journal of Biological Macromolecules, 109, 1292–1301. Elsevier B.V. Retrieved from http://dx.doi.org/10.1016/j.ijbiomac.2017.11.140

Matias, E. F. F., Alves, E. F., Silva, M. K. do N., Carvalho, V. R. de A., Medeiros, C. R., dos Santos, F. A. V., Bitu, V. de C. N., et al. (2015). Phytochemical characterization by HPLC and evaluation of antibacterial and aminoglycoside resistance-modifying activity of chloroform fractions of Cordia verbenacea DC leaf extracts. European Journal of Integrative Medicine, 7(3), 251–257. Elsevier GmbH. Retrieved from http://dx.doi.org/10.1016/j.eujim.2015.03.007

Menezes, I. R. A., Santana, T. I., Varela, V. J. C., Saraiva, R. A., Matias, E. F. F., Boligon, A. A., Athayde, M. L., et al. (2015). Chemical composition and evaluation of acute toxicological, antimicrobial and modulatory resistance of the extract of Murraya paniculata. Pharmaceutical Biology, 53(2), 185–191.

Palmeira, J., Ferreira, S., Souza, J., Almeida, J., Pequeno, A., Figueiredo, M., Arruda, T., et al. (2010). Evaluation of the antimicrobial activity in vitro and determination of minimum the inhibitory concentration (MIC) of hidroalcoholicoc extracts of angico in strains Staphylococcus aureus. Brazilian Journal of Clinical Analyses, 42, 33–37.

Pessoa, W. S., Estevão, L. R. de M., Simões, R. S., Mendonça, F. de S., Evêncio-Luz, L., Baratella-Evêncio, L., Florencio-Silva, R., et al. (2015). Fibrogenesis and epithelial coating of skin wounds in rats treated with angico extract (Anadenanthera colubrina var. cebil). Acta cirurgica brasileira, 30(5), 353–358. SciELO Brasil.

Rocha, E. A. L. de S. S., de Carvalho, A. V. O. R., de Andrade, S. R. A., de Medeiros, A. C. D., Trovão, D. M. de B. M., & Costa, E. M. M. de B. (2013). Potencial antimicrobiano de seis plantas do semiárido paraibano contra bactérias relacionadas à infecção endodôntica. Revista de Ciencias Farmaceuticas Basica e Aplicada, 34(3), 351–355.

Santos, J. S., Marinho, R. R., Ekundi-Valentim, E., Rodrigues, L., Yamamoto, M. H., Teixeira, S. A., Muscara, M. N., et al. (2013). Beneficial effects of Anadenanthera colubrina (Vell.) Brenan extract on the inflammatory and nociceptive responses in rodent models. Journal of ethnopharmacology, 148(1), 218–222. Elsevier.

Segatore, B., Bellio, P., Setacci, D., Brisdelli, F., Piovano, M., Garbarino, J. A., Nicoletti, M., et al. (2012). In vitro interaction of usnic acid in combination with antimicrobial agents against methicillin-resistant Staphylococcus aureus clinical isolates determined by FICI and Δe model methods. Phytomedicine, 19(3–4), 341–347. Elsevier GmbH. Retrieved from http://dx.doi.org/10.1016/j.phymed.2011.10.012

Silva, J. L. da, Mesquita, A. R. C., & Ximenes, E. A. (2009). In vitro synergic effect of β-lapachone and isoniazid on the growth of Mycobacterium fortuitum and Mycobacterium smegmatis. Memorias do Instituto Oswaldo Cruz, 104(4), 580–582.

Silva, S. W. C., Monção, N. B. N., Araújo, B. Q., Arcanjo, D. D. R., Ferreira, J. H. L., Lima Neto, J. S., Citó, A. M. G. L., et al. (2019). Antimicrobial activity of Mimosa caesalpiniifolia Benth and its interaction with antibiotics against Staphylococcus aureus strains overexpressing efflux pump genes. Letters in Applied Microbiology, 69(1), 57–63.

SOUSA, A. K. A. de. (2019). Quaternização Da Goma Do Angico (Anadenanthera Colubrina Var. Cebil (GRISEB.) ALTSCHUL: avaliação da atividade antimicrobiana.

Vieira Pereira, A., Santana, G. M., Góis, M. B., & Sant ’ana, D. M. G. (2015). Tannins obtained from medicinal plants extracts against pathogens: antimicrobial potential. Formatex, 1(December), 228–235.

Weber, C. R., Soares, C. M. L., Lopes, A. B. . D., Silva, T. S., Nascimento, M. S., & Ximenes, E. C. P. A. (2011). Anadenanthera colubrina : A therapeutic potential study. Rev. Bras. Farm., 92(4), 235–244.

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Published

23/05/2021

How to Cite

NASCIMENTO , P. P. S. do .; FERRAZ-CARVALHO, R. de S. .; SILVA, T. S. da .; ARAÚJO, T. A. de S. .; AMORIM, E. L. C. de .; PEREIRA, . M. A. .; CAVALCANTI, I. M. F. Interaction between bark extract of Anadenanthera colubrina var. cebil (Griseb.) Altschul with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). Research, Society and Development, [S. l.], v. 10, n. 6, p. e14210615469, 2021. DOI: 10.33448/rsd-v10i6.15469. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/15469. Acesso em: 26 jul. 2021.

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Agrarian and Biological Sciences