Interaction between bark extract of Anadenanthera colubrina var. cebil (Griseb.) Altschul with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA)




Fabaceae; Staphylococcus aureus; Angico; Caatinga; MRSA.


Anadenanthera colubrina var. cebil (Griseb.) Altschul, a plant often found in areas of the Caatinga in northeastern Brazil, is widely used in unconventional medicine for the treatment of infections and inflammations. Thus, the aim of the present study was to evaluate the antibacterial activity of A. colubrina bark extracts against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates, to investigate if intact and regenerated bark extracts have the same effect against MRSA and to determine the interaction between these extracts and antibiotics. The antibacterial activity was performed by the determination of the minimum inhibitory concentration (MIC) according to the Clinical and Laboratory Standards Institute (CLSI) and the interaction assay was performed by the checkerboard method. A. colubrina extracts showed bacteriostatic activity (MIC = 8-32 mg/L) against MRSA clinical strains and no difference was found in antibacterial activity between intact and regenerated barks, suggesting that even after regeneration, the barks of this species have the same antibacterial activity. Moreover, the in vitro interaction of A. colubrina extracts with ciprofloxacin or erythromycin was additive (FICI = 0.52). Thus, the bark extracts of Anadenanthera colubrina exhibit antibacterial activity and can be used alone or in combination with antibiotics against MRSA clinical isolates.

Author Biographies

Pérola Paloma Silva do Nascimento , Centro Acadêmico de Vitória

Graduada em Ciências Biológicas pela Universidade Federal de Pernambuco (UFPE). Especialista em Ensino de Ciências Biológicas pelo Centro Universitário da Vitória de Santo Antão (UNIVISA). Mestranda pelo Programa de Pós-Graduação em Morfotecnologia pela UFPE.

Rafaela de Siqueira Ferraz-Carvalho, Universidade Federal de Pernambuco

Graduada em Ciências Biológicas pela Universidade Federal de Pernambuco (UFPE). Mestre e doutora pelo Programa de Pós-graduação em Ciências Biológicas da UFPE (PPGCB/UFPE). Pós-doutora pelo programa PNPD CAPES/FACEPE.

Thaís Soares da Silva, Centro Acadêmico de Vitória

Graduada em Ciências Biológicas pela Universidade Federal de Pernambuco (UFPE). Mestre em Educação pela UFPE. Doutoranda no Programa de Pós-Graduação em Ensino da Ciências pela Universidade Federal Rural de Pernambuco (PPGEC/UFRPE).

Thiago Antônio de Sousa Araújo, Universidade Federal de Pernambuco

Graduado em Ciências Biológicas pela Universidade de Pernambuco (UPE). Mestre e Doutor em Ciências Farmacêuticas pela Universidade Federal de Pernambuco (PPGCF/UFPE).

Elba Lúcia Cavalcanti de Amorim, Universidade Federal de Pernambuco

Graduada em Farmácia pela Universidade Federal de Pernambuco (UFPE) e Graduada em Engenharia Química pela Universidade Católica de Pernambuco (UNICAP). Mestre em Ciências Farmacêuticas pela UFPE. Doutorado em Química Orgânica pela Université Pierre et Marie Curie - Paris VI. Professora associada 2 da UFPE.

Marcela Araújo Pereira, Universidade Federal de Pernambuco

Graduada em Biomedicina na Universidade Federal de Pernambuco. Mestre em Ciências Biológicas pela Pós-Graduação de Ciências Biológicas na Universidade Federal de Pernambuco (PPGCB/UFPE). Doutora em Biologia Aplicada à Saúde, pela Universidade Federal de Pernambuco (PPGBAS/UFPE), com doutorado sanduíche (período de 1 ano) na Universidade de Navarra, Espanha.


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How to Cite

NASCIMENTO , P. P. S. do .; FERRAZ-CARVALHO, R. de S. .; SILVA, T. S. da .; ARAÚJO, T. A. de S. .; AMORIM, E. L. C. de .; PEREIRA, . M. A. .; CAVALCANTI, I. M. F. Interaction between bark extract of Anadenanthera colubrina var. cebil (Griseb.) Altschul with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). Research, Society and Development, [S. l.], v. 10, n. 6, p. e14210615469, 2021. DOI: 10.33448/rsd-v10i6.15469. Disponível em: Acesso em: 26 jul. 2021.



Agrarian and Biological Sciences