Development, characterization and application of silver nanoparticles stabilized with sunflower (Helianthus annuus) extract

Authors

DOI:

https://doi.org/10.33448/rsd-v10i6.15533

Keywords:

Nanoparticles silver; Antimicrobial; Helianthus annuus.

Abstract

The use of nanoparticles has been applied in several areas of pharmacology, due to its advantages before absorption, distribution and its effect on disease, especially when it is compose of natural products due to its ability to reduce possible toxic effects of the final product. The study aimed to analyze the effect of Helianthus Annuus extract (AgNP@Ha) in the synthesis of a silver nanoparticle and to evaluate its antimicrobial action against gram-positive, gram-negative bacteria and fungi. The production of the nanoparticle was carried out by stirring over a heated magnetic plate until the complete configuration of the nanoparticle was formed, then, the extract was used to stabilize the nanoparticle, its size was verified by means of UV-VIS. Microbiological analysis was performed on a plate, measuring the size of the inhibitory halo formed. An AgNP@Ha 414 nm that were stabilized using sunflower was obtained with halo formation effect on S. aureus (16 mm), E. coli (12 mm) and P. aeruginosa (10 mm) strains, while AgNPs that were not stabilized with sunflower did not show the inhibitory halo. Through this research it was possible to prove the improvement of pharmacological effect and production efficiency of silver nanoparticles when using sunflower seed extract as a stabilizer The synthesis of silver nanoparticles using the extract of Helianhtus annuus seeds promoted a more environmentally friendly, simple, efficient and low cost approach to obtain nanoparticles, becoming an excellent product with antimicrobial potential to be marketed in addition to serving as a subsidy for further research.

Author Biographies

Taciany Alves Batista Lemos, Centro Universitário UNIFACID/WYDEN

Enfermeira, Mestre em Terapia Intensiva pela SOBRAT.

Deuzuita dos Santos Freitas Viana, Centro Universitário UNIFACID/WYDEN

Dr. Materials Engineering Graduate Program, Federal Institute of Education, Science and Technology of Piauí, Central Campus

Vicente Galber Freitas Viana, Universidade Federal do Piauí

Doutora em Ciências, Mestre em Engenharia Mecânica, Coordenadora das Engenharias da UNIFACID.

Matheus Henrique da Silva Lemos, Universidade Federal do Piauí

Enfermeiro. Mestrando em Ciências e Saúde pela Universidade Federal do Piauí (UFPI)

Girlene Soares de Figueirêdo, Universidade Federal do Piauí

Doutora em Biotecnologia com ênfase em Microbiologia do Centro de Ciências da Saúde da Universidade Federal do Piauí (UFPI)

References

Al-Shabib, N. A, Husain, F. M, Nadeem, M, Khan, M. S, Al-Qurainy, F., Alyousef, A. A., Arshad, M., Khan, A., Khan, J. M., Alam, P., Albalawi, T & Shahzad. (2020). Bio-inspired facile fabrication of silver nanoparticles from in vitro grown shoots of Tamarix nilotica: explication of its potential in impeding growth and biofilms of Listeria monocytogenes and assessment of wound healing ability. RSC Advances, 10(50), 30139-30149.

Atiyeh, B.S., Costagliola, M & Hayek, S. (2007). Effect of silver on burn wound infection control and healing: review of the literature. Burns, 33(2): 139–48.

Bauer, A.W., Kirby, W.M., Sherris, J. C & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45, 493-496.

Brinch, A., Hansen, S. F., Hartmann, N. B, Baun, A. (2016). EU regulation of nanobiocides: challenges in implementing the biocidal product regulation (BPR). Nanomaterials, 6(2), 33.

CLSI. Clinical and Laboratory Standards Institute. (2009). Performance standards for antimicrobial disk susceptibility tests; approved standard-tenth edition M02-A10, 29(1).

Gil, A. C. (2012). Como elaborar projetos de pesquisa. 5. ed. São Paulo: Atlas.

Guo, S, Ge, Y & Jom, K. (2017). A review of phytochemistry, metabolite changes, and medicinal uses of the common sunflower seed and sprouts (Helianthus annuus L.). Chemistry Central Journal, 11(1), 1-10.

Hong, H. R, Kim, J. & Park, C. H. (2018). Facile fabrication of multifunctional fabrics: use of copper and silver nanoparticles for antibacterial, superhydrophobic, conductive fabrics. RSC advances, 8(73), 41782-41794.

Ibrahim, T. A, Ajongbolo, K. F & Aladekoyi, G. (2014). Phytochemical screening and antimicrobial activity of crude extracts of Basella albaand Helianthus annuuson selected food pathogens. Journal of Microbiology and Biotechnology, 3(2), 27–31.

Khan, I., Saeed, K., Khan, I. (2017). Nanoparticles: Properties, applications and toxicities. Arabian Journal of Chemistry, 12(7), 908-931.

Kim, T. H., Kim, M., Park, H. S., Shin, S. U., Gong, M. S. & Kim, H. W. (2012). Size‐dependent cellular toxicity of silver nanoparticles. Journal of biomedical materials research Part A, 100(4),1033-1043.

Lemire, J. A., Harrison, J. J. & Turner, R. J. (2013). Antimicrobial activity of metals: mechanisms, molecular targets and applications. Nature Reviews Microbiology, 11(6), 371-384.

Liu, Z., Yan, J., Miao, Y., Huang, Y. & Liu, T. (2015). Catalytic and antibacterial activities of green-synthesized silver nanoparticles on electrospun polystyrene nanofiber membranes using tea polyphenols. Composites Part B: Engineering, 79(15), 217-223.

Lucky, S. S., Soo, K. C. & Zhang, Y. (2015). Nanoparticles in photodynamic therapy. Chemical reviews, 115(4), 1990-2042.

Oliveira, N. M. S., Resende, M. R., Morales, D. A., Umbuzeiro, G. R. & Boriollo, M. F. G. (2016). In vitro mutagenicity assay (Ames test) and phytochemical characterization of seeds oil of Helianthus/ annuus Linn (sunflower). Toxicology Reports, 3, 733–739.

Park, K., Seo, D. & Lee, J. (2008). Conductivity of silver paste prepared from nanoparticles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 313, 351-354.

Pinto, C. P., Rodrigues, V. D., Pinto, F. P., Pinto, R. P, Uetanabaro, A. P. T., Pinheiro, C. S. R., Gadea, S. F. M., Silva, T. R. S. & Lucchese, A.M. (2013). Antimicrobial activity of Lippia species from the Brazilian semiarid region tradionally used as antiseptic and anti-infective agents. Evidence-Based Complementary and Alternative Medicine, 2013, 1-5.

Prabhu, S. & Poulose, E. K. (2012). Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. International nano letters, 2(1), 1-10.

Qasim, M., Udomluck, N., Chang, J., Park, H. & Kim, K. (2018). Antimicrobial activity of silver nanoparticles encapsulated in poly-N-isopropylacrylamide-based polymeric nanoparticles. International journal of nanomedicine, 13, 235.

Qiao, Z., Han, L., Liu, X., Dai, H., Liu, C., Yan, M., Li, W., Han, W., Li, X., Huang, S. & Gao, B. (2021). Extraction, Radical Scavenging Activities, and Chemical Composition Identification of Flavonoids from Sunflower (Helianthus annuus L.) Receptacles. Molecules, 26(2), 403.

Ravindra, S., Mohan, Y. M., Reddy, N. N. & Raju, K. M. (2010). Fabrication of antibacterial cotton fibres loaded with silver nanoparticles via “Green Approach”. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 367(1-3), 31-40.

Subashini, R. & Rakshitha, S. U. (2012). Phytochemical screening, antimicrobial activity and in vitro antioxidant investigation of methanolic extract of seeds from Helianthus annuus L. Chemical science review and letters, 1(1), 30-34.

Swietnicki, W. (2018). Recent advances in antibacterial drug development. International Journal of Recent Scientific Research, 9, 26501-26505.

Taglietti, A., Fernandez, Y. A. D., Amato, E., Cucca, L., Dacarro, G., Grisoli, P., Necchi, V., Pallavicini, P., Pasotti, L. & Patrini, M. (2012). Antibacterial activity of glutathione-coated silver nanoparticles against gram positive and gram negative bacteria. Langmuir, 28(21), 8140-8148.

Tang, D., Dong, Y., Ren, H., Li, L. & He, C. (2014). A review of phytochemistry, metabolite changes, and medicinal uses of the common food mung bean and its sprouts (Vigna radiata). Chemistry Central Journal, 8(1), 1-9.

Tang, S. & Zheng, J. (2018). Antibacterial activity of silver nanoparticles: structural effects. Advanced healthcare materials, 7(13), 1701503.

Thierry, B. (2009). Drug nanocarriers and functional nanoparticles: applications in cancer therapy. Current drug delivery, 6(4), 391-403.

Published

30/05/2021

How to Cite

LEMOS, T. A. B. .; VIANA, D. dos S. F. .; VIANA, V. G. F. .; LEMOS, M. H. da S. .; FIGUEIRÊDO, G. S. de . Development, characterization and application of silver nanoparticles stabilized with sunflower (Helianthus annuus) extract. Research, Society and Development, [S. l.], v. 10, n. 6, p. e11710615533, 2021. DOI: 10.33448/rsd-v10i6.15533. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/15533. Acesso em: 20 jun. 2021.

Issue

Section

Health Sciences