Comparison of general pathological processes in the pulmonary epithelium of spontaneously hypertensive rats submitted to high intensity interval training

Authors

DOI:

https://doi.org/10.33448/rsd-v10i6.15602

Keywords:

High Intensity Interval Training; Hypertension ; Rats ; Lung.

Abstract

The aim was to compare and morphologically quantify the pulmonary epithelium of hypertensive rats exposed to high intensity interval training (HIIT). This is an experimental study, in which male Wistar Kyoto rats of the spontaneously hypertensive strain (SHR) (n = 16) were divided into Group 01 (Spontaneously hypertensive sedentary controls (SHR, n = 8)) and Group 02 (Spontaneously trained hypertensive patients (SHR + T, n = 8)). The exercise was performed on a mat adapted for rodents, for 50 minutes / day, 5 days / week for a total of 8 weeks. The intervals between running shots were 3 minutes with 60% of the exhaustion speed. After 8 weeks of training, the rats were euthanized and performed an autopsy to remove the lungs. The tissues, after making the slides, were stained with Hematoxylin and Eosin (HE) to assess the inflammatory infiltrate by quantifying the peribronchial region using a semi-quantitative method. A significant reduction in the weight of SHR + T rats was observed when compared to SHR without intervention. In the morphometric comparison between the areas of the airway, pulmonary artery, pulmonary artery lumen and the alveolar area, no statistically significant differences were observed. Through this study it was possible to prove the effectiveness of HIIT training in physical conditioning, weight loss and with a positive impact on the control of arterial hypertension, however in relation to the improvement of the lung parenchyma observed a difference in the results. We think that a longer period of exercise is necessary for there to be such a change in epithelial and parenchymal changes.

References

Adolfo, J. R., Dhein, W., & Sbruzzi, G. (2019). Diferentes intensidades de exercício físico e capacidade funcional na DPOC: revisão sistemática e meta-análise. Jornal Brasileiro de Pneumologia, 45(6).

Brasil. Ministério da Saúde. Departamento de Informática do Sistema Único de Saúde (DATASUS) [online]. Brasília [s.d.]. Disponível em: http:// www.datasus.gov.br. Acessado em 25 de março de 2021.

Blowey, D. L. (2016). Diuretics in the treatment of hypertension. Pediatric nephrology, 31(12), 2223-2233.

Buchheit, M., & Laursen, P. B. (2013). High-intensity interval training, solutions to the programming puzzle. Sports medicine, 43(5), 313-338.

Camillo, C. A., de Moraes Laburu, V., Gonçalves, N. S., Cavalheri, V., Tomasi, F. P., Hernandes, N. A., ... & Pitta, F. (2011). Improvement of heart rate variability after exercise training and its predictors in COPD. Respiratory medicine, 105(7), 1054-1062.

Carnieli, D. S. (2010). Inflamação e remodelamento pulmonar em camundongos com sensibilização alérgica em diferentes idades: um estudo comparativo (Doctoral dissertation, Universidade de São Paulo).

Christensen, P. M., Jacobs, R. A., Bonne, T., Flück, D., Bangsbo, J., & Lundby, C. (2016). A short period of high-intensity interval training improves skeletal muscle mitochondrial function and pulmonary oxygen uptake kinetics. Journal of Applied Physiology, 120(11), 1319-1327.

Costa, E. C., Hay, J. L., Kehler, D. S., Boreskie, K. F., Arora, R. C., Umpierre, D., ... & Duhamel, T. A. (2018). Effects of high-intensity interval training versus moderate-intensity continuous training on blood pressure in adults with pre-to established hypertension: a systematic review and meta-analysis of randomized trials. Sports Medicine, 48(9), 2127-2142.

Dalpiaz, M. R., Dorneles, G. P., Souza, M. P., Peres, A., Monteiro, M. B., & Souza, M. P. (2016). Treinamento Intervalado de Alta Intensidade: quebrando paradigmas na reabilitação cardiovascular. Revista Brasileira de Prescrição e Fisiologia do Exercício (RBPFEX), 10(57), 16-28.

de Vargas, L. D. S., das Neves, B. H. S., Roehrs, R., Izquierdo, I., & Mello-Carpes, P. (2017). One-single physical exercise session after object recognition learning promotes memory persistence through hippocampal noradrenergic mechanisms. Behavioural brain research, 329, 120-126.

Elmi-Sarabi, M., Deschamps, A., Delisle, S., Ased, H., Haddad, F., Lamarche, Y., ... & Denault, A. Y. (2017). Aerosolized vasodilators for the treatment of pulmonary hypertension in cardiac surgical patients: a systematic review and meta-analysis. Anesthesia & Analgesia, 125(2), 393-402.

Ferreira, N. Z., de Souza, F. L. A., Mariano, T. B., Carrara, B., Collegio, G., Molinari, A. O., ... & Pacagnelli, F. L. (2017). EFEITO HIPOTENSOR DO EXERCÍCIO INTERVALADO DE ALTA INTENSIDADE EM ANIMAIS ESPONTANEAMENTE HIPERTENSOS. In Colloquium Vitae. ISSN: 1984-6436 (Vol. 9, No. 3, pp. 31-35).

Garcia-Lunar, I., Blanco, I., Fernández-Friera, L., Prat-Gonzàlez, S., Jordà, P., Sánchez, J., ... & García-Álvarez, A. (2020). Design of the β3-adrenergic agonist treatment in chronic pulmonary hypertension secondary to heart failure trial. Basic to Translational Science, 5(4), 317-327.

Guio de Prada, V., Ortega, J. F., Morales-Palomo, F., Ramirez-Jimenez, M., Moreno-Cabañas, A., & Mora-Rodriguez, R. (2019). Women with metabolic syndrome show similar health benefits from high-intensity interval training than men. PloS One, 14(12), e0225893.

Hannan, A. L., Hing, W., Simas, V., Climstein, M., Coombes, J. S., Jayasinghe, R., ... & Furness, J. (2018). High-intensity interval training versus moderate-intensity continuous training within cardiac rehabilitation: a systematic review and meta-analysis. Open access journal of sports medicine, 9, 1.

Haram, P. M., Kemi, O. J., Lee, S. J., Bendheim, M. Ø., Al-Share, Q. Y., Waldum, H. L., ... & Wisløff, U. (2009). Aerobic interval training vs. continuous moderate exercise in the metabolic syndrome of rats artificially selected for low aerobic capacity. Cardiovascular research, 81(4), 723-732.

Issa, A. F. C., Nascimento, B. R., Correa Filho, H., Vieira, M. L. C., Barroso, W. K. S., Rodrigues, C. S., ... & Mota-Gomes, M. A. (2021). Diretrizes Brasileiras de Hipertensão Arterial–2020. Arq Bras Cardiol, 116(3), 516-658.

Ito, S. (2019). High-intensity interval training for health benefits and care of cardiac diseases-the key to an efficient exercise protocol. World Journal of Cardiology, 11(7), 171.

Laverack, G. (2017). The challenge of behaviour change and health promotion. Challenges, 8(2), 25.

Liu, J. X., Zhu, L., & Deng, J. M. (2019). The effects of high-intensity interval training versus moderate-intensity continuous training on fat loss and cardiometabolic health in pediatric obesity: A protocol of systematic review and meta-analysis. Medicine, 98(10).

Malachias, M. V. B., Póvoa, R. M. S., Nogueira, A. R., Souza, D., Costa, L. S., & Magalhães, M. E. (2016). 7ª Diretriz Brasileira de Hipertensão Arterial: Capítulo 3-Avaliação Clínica e Complementar. Arquivos Brasileiros de Cardiologia, 107(3), 14-17.

Maillard, F., Vazeille, E., Sauvanet, P., Sirvent, P., Combaret, L., Sourdrille, A., ... & Boisseau, N. (2019). High intensity interval training promotes total and visceral fat mass loss in obese Zucker rats without modulating gut microbiota. PLoS One, 14(4), e0214660.

Malta, D. C., Moura, L. D., Prado, R. R. D., Escalante, J. C., Schmidt, M. I., & Duncan, B. B. (2014). Mortalidade por doenças crônicas não transmissíveis no Brasil e suas regiões, 2000 a 2011. Epidemiologia e Serviços de Saúde, 23, 599-608.

Mills, K. T., Stefanescu, A., & He, J. (2020). The global epidemiology of hypertension. Nature Reviews Nephrology, 16(4), 223-237.

Mirghani, S. J., Seydyousefi, M., Pekkala, S., Sharifian, S., & Beyshami, G. (2019). Shorter recovery time following high-intensity interval training induced higher body fat loss among overweight women. Sport Sciences for Health, 15(1), 157-165.

Mullick, A. E., Yeh, S. T., Graham, M. J., Engelhardt, J. A., Prakash, T. P., & Crooke, R. M. (2017). Blood pressure lowering and safety improvements with liver angiotensinogen inhibition in models of hypertension and kidney injury. Hypertension, 70(3), 566-576.

Smith-Ryan, A. E., Melvin, M. N., & Wingfield, H. L. (2015). High-intensity interval training: Modulating interval duration in overweight/obese men. The Physician and sportsmedicine, 43(2), 107-113.

Spruit, M. A., Singh, S. J., Garvey, C., ZuWallack, R., Nici, L., Rochester, C., ... & Wouters, E. F. (2013). An official American Thoracic Society/European Respiratory Society statement: key concepts and advances in pulmonary rehabilitation. American journal of respiratory and critical care medicine, 188(8), e13-e64.

Vilela-Martin, J. F., Yugar-Toledo, J. C., Rodrigues, M. D. C., Barroso, W. K. S., Carvalho, L. C. B. S., González, F. J. T., ... & Póvoa, R. M. D. S. (2020). Posicionamento Luso-Brasileiro de Emergências Hipertensivas–2020. Arquivos Brasileiros de Cardiologia, 114(4), 736-751.

Yamada, A. T. T., Lavras, C., & DEMUNER, M. (2011). Manual de orientação clínica: hipertensão arterial sistêmica (HAS). São Paulo: SES.

Published

27/05/2021

How to Cite

PIRES, A. N. S. .; MELLO, F. de A. .; COSTA, S. M.; CHAGAS, D. .; CAVALIERI, G. V. .; SALGE, A. K. M. .; FAVARETO, A. P. A. .; SILVA, D. T. de R. e .; PACAGNELLI, F. L.; ROSSI, R. C. . Comparison of general pathological processes in the pulmonary epithelium of spontaneously hypertensive rats submitted to high intensity interval training. Research, Society and Development, [S. l.], v. 10, n. 6, p. e19310615602, 2021. DOI: 10.33448/rsd-v10i6.15602. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/15602. Acesso em: 24 jun. 2021.

Issue

Section

Health Sciences