Can daily consumption of a nephroprotective homeopathic product protect the kidneys from gentamicin toxicity?
DOI:
https://doi.org/10.33448/rsd-v10i7.16592Keywords:
Gentamicin; Homeopathic; Renal failure.Abstract
The objective of this work was to determine whether preventive consumption of a homeopathic product via drinking water would protect mouse kidneys from a challenge with the nephrotoxic antibiotic gentamicin. We used 40 Swiss mice divided into four groups with ten animals each. The homeopathic product was supplied through water for 30 days in a preventive manner and gentamicin for 10 days to induce an experimental renal failure. The groups were as follows: Negative-CT (homeopathic and gentamicin was not provided), Positive-CT (did not receive homeopathic, but received gentamicin 40 mg/kg), T2 (received 0.002 ml of the product per animal/day, and received gentamicin 40 mg/kg), and T4 (0.004 ml of the product per animal/day and received gentamicin 40 mg/kg). On days 12 and 20, blood and tissue samples were collected from five animals in each group. No histopathological lesions were found in mouse kidneys. However, levels of thiobarbituric acid reactive substances, reactive oxygen species, and nitrite/nitrate ratios in the kidney of the Positive-CT group were higher compared to the other groups. As for glutathione S-transferase, on the 20th day, the groups that used the homeopathic product (T2 and T4) had higher activities than the positive TC. Therefore, the results suggest that prophylactic consumption of the hepatoprotective homeopathic product can decrease lipid peroxidation, nitrous stress, and oxidative stress at the renal level when consecutive doses of gentamicin induce insufficiency.
References
Birben, E., Sahiner, U. M., Sackesen, C. et al. (2012). Estresse Oxidativo e Defesa Antioxidante. World Allergy Organ J 5,9–19.
De Paula Coelho, C., Motta, P. D., Petrillo, M., de Oliveira I. R., Dalboni, L. C., Santana, F. R. & Bonamin, L. V. (2017). O medicamento homeopático Cantharis modula a cistite induzida por E. coli uropatogênica (UPEC) em camundongos suscetíveis. Cytokine, 92, 103-109.
Duarte, F. Pessoa, E. A., Reis, L. A., et al. (2016) Priming previne an insuficiência renal aguda nefrotóxica através da estimulação do mecanismo de defesa antioxidante. J Bras Nefrol. 161-172.
Fatemi, F., Allameh, A., Dadkhah A., Forouzandeh, M., Kazemnejad, S., & Sharif, R. (2006). Changes in hepatic cytosolic glutathione-S-transferase activity and expression of its class-P during prenatal and postnatal period in rats treated with aflatoxin B1. Arch. Toxicol. 80:572–579.
Gius, D., Botero, A., Shah, S., & Curry, H. A. (1999). Intracellular oxidation/reduction status in the regulation of transcription factors NF-κB and AP-1. Toxicol Lett. 106: 93–106.
Gutteridge, J. M. C., & Halliwell, B. (2018). Mini-Review: Oxidative stress, redox stress or redox success? Biochemical and Biophysical Research Communications, 502, 183-186.
Habig, W. H., Pabst, M. J., & Jakoby, W. B. (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130–7139.
Halliwell, B., Gutteridge, J. M. C. (2007). Free radicals in biology and medicine, (4th ed.), Oxford University Press,
Jaguezeski, A. M., Glombowsky, P., Galli, G. M., da Rosa, G., Araújo, D. N., Campigotto, G., Horn, V. W., Sareta, L., Mendes, R. E., & Da Silva, A.S. (2020). Daily consumption of a homeopathic product decreases intestinal damage and stool bacterial counts in mice challenged with Escherichia coli, Microbial Pathogenesis.
Jentzsch, A. M., Bachmann, H., Fürst, P., & Biesalski, H. K. (1996). Improved analysis of malondialdehyde in human body fluids. Free Radical Bio Med. 20:251- 256.
Jyothilakshmi, V., Thellamudhu, G., Chinta, R., Alok, K., Anil, K., Debadatta, N., & Kalaiselvi, P. (2014). Efeito antioxidante benéfico da preparação homeopática de Berberis vulgarisina no alívio do estresse oxidativo em urolitíase experimental. Forschende Komplementärmedizin/Research in Complementary Medicine, 21 (1), 7–12.
Lopes, C. R., Falkowski, G. J. S., Brustolin, C. F., Massini, P. F., Ferreira, É. C., Moreira, N. M., & de Araújo, S. M. (2016). A medicação altamente diluída reduz o parasitismo e a inflamação dos tecidos em camundongos infectados pelo Trypanosoma cruzi. Homeopatia, 105 (2), 186–193.
Nagajyothi, F., Zhao, D.; Weiss, L. M., & Tanowitz, H. B. (2012). Curcumin treatment provides protection against Trypanosoma cruzi infection. Parasitology Research, 110, 2491-2499.
Miranda, K. M., Espey, M. G., & Wink, D. A. (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide, 5, 62-71.
Motta, T. V. (2003). Bioquímica Clínica para laboratório. Editora Robe.
Nelson, D. P., & Kiesow, L. A. (1972). Enthalpy of decomposition of hydrogen peroxide by catalase at 25° C (with molar extinction coefficients of H2O2 solutions in the UV). Analytical Biochemistry, 49(2), 474–478.
Ohkawa, H., Ohishi, N., & Yagi, K. (1978). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry. 95: 351-358.
Oliveira, V., & Cunha R. et al. (2001). Interferência do intervalo de administração da droga sobre a nefrotoxicidade da gentamicina em ratos. Revista da Escola de Enfermagem da Usp.
Pöppl, Á. G., González, F. H. D., & da Silva, S. C. (2004). Alterações Clínico-laboratoriais em Transtornos Renais de Cães (Canis familiaris). Medvep - Revista Científica de Medicina Veterinária - Pequenos Animais e Animais de Estimação, p. 1-7.
Prado Neto, J. A., Perazzo, F. F., Cardoso, L. G. V., Bonamin, L. V., & Carvalho, J. C. T. (2004). Action of Causticum in inflammatory models. Homeopathy, 93, 12-16.
Rufato, F. H. F., de Rezende Lago, N. C. M., & de Marchi, P. G. F. (2011). Insuficiência renal em cães e gatos. Interdisciplinar. Revista Eletrônica da Univar, Mato Grosso, p. 167-173.
Sakamoto, M. I., Murakami, A. E., Fernandes, A. M., Ospina-Rojas, I. C., Nunes, K. C., & Hirata, A. K. (2018). Performance and serum biochemical profile of Japanese quail supplemented with silymarin and contaminated with aflatoxin B1. Poultry Science 97(1):159–166.
Scanes, C. G. (2015). Sturkie's avian physiology. Elsevier.
Stevens, L. A., & Levey, A. S. (2005). Measurement of kidney function. Med Clin North Am. 89(3):457-73.
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Copyright (c) 2021 Giovana Carolina Machado; Patricia Glombowsky; Gabriela Miotto Galli; Bianca Fagan Bissacotti; Priscila Marquezan Copetti; Leticia Druzian; Vera Maria Morsch; Ricardo Evandro Mendes; Aleksandro Schafer da Silva
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