Immunoxpression of MMPs -9, -13 and TIMP-3 after Using Etch-and-rinse Adhesive
DOI:
https://doi.org/10.33448/rsd-v10i9.17315Keywords:
Dentin; Matrix Metalloproteinase 9; Matrix Metalloproteinase 13; Tissue Inhibitor of Metalloproteinase-3; Dentin-bonding agents.Abstract
The aim of the present study was to determine the expression of MMP-9, MMP-13 and TIMP-3 after using the XP Bond ™ (Dentsply) dentin-bonding agent on 21 human teeth. Class I deep cavities were prepared and restored. After 01, 07, 14, 21, 30, 90 and 120 days, the teeth were extracted and processed for an immunohistochemical assessment (n=3). Immunohistochemical staining was performed using the monoclonal antibody anti-MMP-9, and polyclonal antibodies anti-MMP-13 and anti-TIMP-3. The immunoreactivity of metalloproteinases 9 and 13 was considered intense and moderate in the first storage intervals, before decreasing over time. In contrast, the immunoreactivity of TIMP-3 was considered absent in dentin and weak in pulp in the first time intervals, and completely absent in the subsequent intervals. MMP-9 expression in dentin and in pulp was prevalent in the dentinal tubules and odontoblastic layer, respectively. MMP-13 expression in dentin and in pulp was predominant in the pre-dentine region and odontoblastic layer, respectively. TIMP-3 expression was absent in dentin and predominantly located in the odontoblastic layer for pulp. In conclusion, the expression patterns of MMP-9, MMP-13 and TIMP-3 altered in the different time intervals studied. The increased expression of TIMP-3 in the first time intervals could suggest a synchronous response to the increased expression of MMPs in the same periods. The results confirmed the low performance of TIMP-3 in the physiological processes of the pulp-dentin complex for the conditions and materials tested herein.
References
Accorsi-Mendonça, T., Silva, E.J.N.L., Marcaccini, A.M., Gerlach, R.F., Duarte, K.M.R., Pardo, A.P.S., Line, S.R.P., Zaia, A.A. (2013). Evaluation of gelatinases, tissue inhibitor of matrix metalloproteinase-2, and myeloperoxidase protein in healthy and inflamed human dental pulp tissue. J Endod, 39, 879–882.
Brew, K., Dinakarpandian, D., Nagase, H. (2000). Tissue inhibitors of metalloproteinases: evolution, structure and function. Biochim Biophys Acta, 1477 (1-2), 267–283.
Brew, K., Nagase, H. (2010). The tissue inhibitors of metalloproteinases (TIMPs): An ancient family with structural and functional diversity. Biochim Biophys Acta, 1803(1), 55–71.
De Souza Costa, C.A., Hebling, J., Scheffel, D.L., Soares, D.G., Basso, F.G., Ribeiro, A.P. (2014). Methods to evaluate and strategies to improve the biocompatibility of dental materials and operative techniques. Dent Mater, 30(7), 769–784.
Estrela, C. (2001). Metodologia científica: ensino e pesquisa em odontologia. In Metodologia científica: ensino e pesquisa em odontologia (pp. 483-483).
Franco, C.F., Souza, A.C.D., Costa, J.E., D‘Almeida, P.M. (2009). MMP-8 and periodontal disease. R. Periodontia, 19(4), 51–60.
Gomis-Ruth, F.X., Maskos, K., Betz, M., Bergner, A., Huber, R., Suzuki, K., Yoshida, N., Nagase, H., Brew, K., Bourenkovk, G.P., Bartunikk, H., Bode, W. (1997). Mechanism of inhibition of the human matrix metalloproteinase stromelysin-1 by TIMP-1. Nature, 389(6646), 77–81.
Hebling, J., Ribeiro, A.P.D., Costa, C.A.S. (2010). Relationship between dental materials and the dentin-pulp complex. Rev Odontol Bras Central, 18(48), 1–9.
Hemmann, S., Graf, J., Roderfeld, M., Roeb, E. (2007). Expression of MMPs and TIMPs in liver fibrosis – a systematic review with special emphasis on anti-fibrotic strategies. J Hepatol, 46(5), 955–975.
Ishiguro, K., Yamashita, K., Nakagaki, H., Iwata, K., Hayakawa, T. (1994). Identification of tissue inhibitor of metalloproteinases-1 (TIMP-1) in human teeth and its distribution in cementum and dentine. Arch Oral Biol, 39(4), 345–349.
Khokha, R., Murthy, A., Weiss, A. (2013). Metalloproteinases and their natural inhibitors in inflammation and immunity. Nature, 13(9), 649–665.
Knauper, V., Cowell, S., Smith, B., Lopez-Otin, C., O'shea, M., Morris, H., Zardi, L., Murphy, G. (1997). The Role of the C-terminal Domain of Human Collagenase-3 (MMP-13) in the Activation of Procollagenase-3, Substrate Specificity, and Tissue Inhibitor of Metalloproteinase Interaction. J. Biol. Chem, 272(12), 7608–7616.
Li, H., Li, T., Li, X., Zhang, Z., Li, P., Li, Z. (2015) Morphological effects of MMPs inhibitors on the dentin bonding. Int J Clin Exp Med, 8(7), 10793–10803.
Loreto, C., Galanti, C., Musumeci, G., Rusu, M.C., Leonardi, R. (2014). Immunohistochemical analysis of matrix metalloproteinase-13 in human caries dentin. Eur J Histochem, 58(1), 47–51.
Lovász, B.V., Lempel, E., Szalma, J., Sétáló, G., Vecsernyés, M., & Berta, G. (2021). Influence of TEGDMA monomer on MMP-2, MMP-8, and MMP-9 production and collagenase activity in pulp cells. Clinical Oral Investigations, 25(4), 2269–2279.
Masioli, M.A., Dias, K.R.H.C., Vargas, P.R.M., Rosa, R. (2007). Microscopic evaluation of exposed human pulp, following direct pulp capping with dentine adhesive and calcium hydroxide-based. UFES Rev. Odontol, 9(3), 4–11.
Mazzoni, A., Papa, V., Nato, F., Carrilho, M., Tjäderhane, L., Ruggeri Jr, A., Gobbi, P., Mazzotti, G., Tay, F.R., Pashley, D.H., Breschi, L. (2011). Immunohistochemical and biochemical assay of MMP-3 in human dentine. J Dent, 39, 231–237.
Mazzoni, A., Carrilho, M., Papa, V., Tjäderhane, L., Gobbi, P., Nucci, C., ... & Breschi, L. (2011). MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: biochemical and immunohistochemical analysis. Journal of Dentistry, 39(7), 470–477.
Mazzoni, A., Pashley, D. H., Tay, F. R., Gobbi, P., Orsini, G., Ruggeri Jr, A., ... & Breschi, L. (2009). Immunohistochemical identification of MMP‐2 and MMP‐9 in human dentin: correlative FEI‐SEM/TEM analysis. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials, 88(3), 697–703.
Mazzoni, A., Tjäderhane, L., Checchi, V., Di Lenarda, R., Salo, T., Tay, F. R., ... & Breschi, L. (2015). Role of dentin MMPs in caries progression and bond stability. Journal of dental research, 94(2), 241–251.
Nagase, H., Visse, R., Murphy, G. (2006). Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc Res, 69(3), 562–573.
Nassar, M., Hiraishi, N., Shimokawa, H., Tamura, Y., Otsuki, M., Kasugai, S., Ohya, K., Tagami, J. (2014). The inhibition effect of non-protein thiols on dentinal matrix metalloproteinase activity and HEMA cytotoxicity. J Dent, 42(3), 312–318.
Pashley, D.H., Tay, F.R., Yiu, C., Hashimoto, M., Breschi, L., Carvalho, R.M., Ito, S. (2004). Collagen degradation by host-derived enzymes during aging. J Dent Res, 83(3), 216–221.
Sabatini, C., Pashley, D.H. (2014). Mechanisms Regulating the Degradation of Dentin Matrices by Endogenous Dentin Proteases and their Role in Dental Adhesion. A Review. Am J Dent, 27(4), 203–214.
Sulkala, M., Larmas, M., Sorsa, T., Salo, T., Tjäderhane, L. (2002). The localization of matrix metalloproteinase-20 (MMP-20, enamelysin) in mature human teeth. J Dent Res, 81(9), 603–607.
Tahmiščija, I., Radović, S., Jukić-Krmek, S., Konjhodžić, A., Hasić-Branković, L., Džanković, A., & Korać, S. (2019). Immunohistochemical expression of matrix metalloproteinase MMP-2 and MMP-9 in healthy and inflamed human dental pulp. Folia Medica Facultatis Medicinae Universitatis Saraeviensis, 53(1), 3–9.
Tezvergil-Mutluay, A., Agee, K. A., Hoshika, T., Carrilho, M., Breschi, L., Tjäderhane, L., ... & Pashley, D. H. (2010). The requirement of zinc and calcium ions for functional MMP activity in demineralized dentin matrices. Dental Materials, 26(11), 1059–1067.
Tezvergil-Mutluay, A., Mutluay, M., Seseogullari-Dirihan, R., Agee, K. A., Key, W. O., Scheffel, D. L. S., ... & Pashley, D. H. (2013). Effect of phosphoric acid on the degradation of human dentin matrix. Journal of dental research, 92(1), 87–91.
Visse, R., Nagase, H. (2003). Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry. Circ Res, 92, 827–839.
Zitka, O., Kukacka, J., Krizkov, S., Huska, D., Adam, V., Masarik, M., Prusa, R., Kizek, R. (2010). Matrix Metalloproteinases. Curr Med Chem, 17(31), 3751–3768.
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Copyright (c) 2021 Laís Maciel Costa; Ariela Vilela Rizuto; Luciana Sarmento de Mendonça; Marianne de Vasconcelos Carvalho; Márcia Maria Fonseca da Silveira; Ana Paula Veras Sobral
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