Validation of the determination of β-hydroxybutyrate on dipstick using a portable glucometer in sheep of Dorper and White Dorper

Authors

DOI:

https://doi.org/10.33448/rsd-v10i10.17736

Keywords:

Ketone bodies; Sheep; Diagnosis.

Abstract

Due to the growth of sheep breeding and the increase in demand for their products, there is the need to invest in the health of these animals. Thus, it becomes essential to use diagnostic tools for metabolic diseases like toxemia of pregnancy. This research aims to validate the diagnostic technique in reagent strip for β-hydroxybutyrate (BHB) in human portable glucose in sheep of Dorper and White Dorper. 111 otherwise healthy sheep, 79 of 32 Dorper and White Dorper were used in different production phases, which were empty (n=44), patients (n=37) and recently calved (n=30). Blood collection was performed by puncturing the jugular vein, with vacuum collection system, without anticoagulant tube. Instantly, was performed to determine the BHB test strip by the method of using the portable glucose meter. The determination of BHB in serum was performed in an automatic biochemical analyzer. Analysis, using the Kolmogorov-Smirnov test (p<0.05), there was a non-parametric distribution of the data, they are arranged in a paired manner, to which the same individual was examined by two techniques, the test was chosen McNemar to verify the difference between hypothesis testing. In addition, we performed the calculation of Kappa coefficient (95%) to check the reproducibility of the tests, combined with sensitivity and specificity calculations, considering the laboratory test as the gold standard. There was no significant difference between the results of dipstick to ketone bodies and biochemistry laboratory analysis, considering the total of 111 animals. In this analysis, the statistical resulted in a Kappa Index of 85%, with a sensitivity of the reagent strip 93% and specificity of 96%. In animals divided into different breeding groups, because there is no disagreement results, it is considered the Kappa index 100% and thus no possibility of calculating the sensitivity and specificity. This was only possible in newly calved classification of animals. In this category, the McNemar test (p=0.3173) with 95% demonstrated a Kappa coefficient 80.6%, with 93% sensitivity and 87% specificity. The recently calved sheep in this category groups have paired results and thus presented a Kappa coefficient of 80.6%, with 93% sensitivity and 87% specificity. The test reagent strip for β-hydroxybutyrate in human portable blood glucose meter is considered excellent and reliable and can be used as a diagnostic tool in sheep.

References

Araújo, C. A. S. C., Minervino, A. H. A., Sousa, R. S., Oliveira, F. L. C., Rodrigues, F. A. M. L., Mori, C. S. & Ortolani, E. L. (2020). Validation of a handheld β-hydroxybutyrate acid meter to identify hyperketonaemia in ewes. Biochemistry, Biophusics and Molecular Biology, 8:8933-8942.

Chagas, A. C. S., Oliveira, M. C. S., Carvalho, C. O. & Molento, M. B. (2007). Método Famacha©: Um recurso para o controle de verminose em ovinos. Circular técnica 52, 8p. Embrapa Pecuária Sudeste.

Crilly, J. P., Phythian, C. & Evans, M. (2021). Advances in managing pregnancy toxaemia in sheep. In Practice, 43:79-94.

Contreras, P., Wittwer, F. & Böhmwald H. (2000). Uso dos perfis metabólicos no monitoramento nutricional de ovinos. In: González F.H.D., Barcellos J.O., Ospina H., Ribeiro L.A.O. Perfil metabólico em ruminantes: seu uso em nutrição e doenças nutricionais. Gráfica da Universidade Federal do Rio Grande do Sul, Porto Alegre. 75-88.

Doré, V., Dubuc, J., Bélanger A. M. & Buczinski S. (2013). Short communication: Evaluation of the accuracy of na eletronic on-farm test to quantify blood β-hydroxybutyrate concentration in dairy goats. Journal Dairy Sciencex, 96(7), 4505-4507.

Duffield, T. (2000). Subclinical ketosis in lactating dairy cattle. Veterinary Clinics of North America. Food Animal Practice, 16:231-253.

Eurodiagnóstico (2015). Beta-Hydroxybutyrate (beta-HB) Assay Kit.

Hernández, J., Benedito, J. L., & Castillo, C. (2020). Relevance of the study of metabolic profiles in sheep and goat flock. Spanish Journal of Agricultural Research, 18:1-14.

Iwersen, M., Kklein-Jobstl, D., Pichler, M., Roland, L., Fildlschuster, B., Scchwendenwein, I. & Drillich, M. (2013). Comparason of 2 electronic cowside tests to detect subclinical ketosis in dairy cows and the influence of the temperature and type of blood sample on the tests results. American Dairy Science Association, 96(12), 7719-7730.

Karagiannis, I., Panousis, N., Kiossis, E., Tsakmakidis, I., Lafi, S., Arsenos, G., Boscos, S.C. & Brozos, C. (2014). Associations of pre-lambing body condition score and sérum β-hydroxybutyric acid and non-esterified fatty acids concentration with peroparturient health of chios dairy ewes. Small Ruminant Research, 120(1), 164-173.

Nasciutti, N. R., Tsuruta, S. A., Oliveira, R. S. B. R., Bisinoto, M., Headley, S. A., Mundim, A. V., Noleto, P. G. & Sault, J. P. E. (2012). Perfil metabólico em ovelha Santa Inês, com baixo escore de condição corporal, no periparto. Boletim de Indústria Animal, 69(2), 137-145.

Oliveira, R. P. M., Maduro, A. H. P., Oliveira, F. F. & Lima, E. S. (2014). Perfil metabólico de ovelhas Santa Inês em diferentes fases de gestação criadas em sistema semi-intensivo no estado do Amazonas. Ciência Animal Brasileira, 15(1), 81-86.

Panousis, N., Kritsepi, M., Karagiannis, I., Kalaitzakis, E., Lafi, S. & Brozos, C. (2011). Evaluation of precision Xceed for on-site monitoring of blood β-hydroxybutyric acid and glucose in dairy cows. Jounal of the Ellenic Veterinary Medical Society, 62(2), 109-117.

Panousis, N., Brozos, C., Karagiannis, I., Giadinis, N. D., Lafi, S. & Kritsepi-Konstantinou, M. (2012). Evaluation of Precision Xceed® meter for on-site monitoring of blood β-hydroxybutyric acid and glucse concentration in dairy sheep. Research in Veterinary Science, 93, 435-439.

Pereira M. G. (1995). Epidemiologia: teoria e prática. Rio de Janeiro. 363p. Editora Guanabara Koogan.

Pichler, M., Damberger, A., Arnholdt, T., Schwendenwein, J., Gasteiner, J., Drillich, M. & Iwersen, M. (2014). Evaluation of 2 eletronic handheld devices of diagnosis of cetonemia and glycemia and dairy goats. Journal Dairy Science, 97(12), 7538-7546.

Santos, F. C. O., Mendonça, C. L., Silva-Filho, A. P., Carvalho, C. C., Soares, P. C. & Afonso J. A. B. (2011). Indicadores bioquímicos e hormonais de casos naturais de toxemia da prenhez em ovelhas. Pesquisa Veterinária Brasileira, 31(11), 974-980.

Silva, N. C. D., Bonfá, H. C. & Borges, I. (2011). Cetose em Pequenos Ruminantes. Revista Eletrônica Nutritime, 8(6), 1647-1657.

Simões, P. B. A., Bexiga, R., Lamas, L. P., & Lima, M. S. (2020). Pregnancy Toxaemia in Small Ruminants. In Advances in Animal Health. Medicine and Production, pp. 541-556. Springer, Cham.

Voyvoda, H. & Erdogan H. (2010). Use of hand-held meter for detecting subclinical ketosis in dairy cows. Research in Veterinary Science, 89(3), 344-351.

Published

07/08/2021

How to Cite

RIBEIRO, F. A. .; ALMEIDA, I. C. de .; SANTOS, T. Ávila dos .; DENADAI, L. de B. .; ALMEIDA, S. L. H. de .; SILVA, I. J. F.; GUERSON, Y. B. .; MADUREIRA, A. P.; PAULA, H. de .; SIQUEIRA, J. B. .; BARIONI, G. Validation of the determination of β-hydroxybutyrate on dipstick using a portable glucometer in sheep of Dorper and White Dorper . Research, Society and Development, [S. l.], v. 10, n. 10, p. e157101017736, 2021. DOI: 10.33448/rsd-v10i10.17736. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/17736. Acesso em: 20 oct. 2021.

Issue

Section

Agrarian and Biological Sciences