Araucaria angustifolia (bert.) Otto kuntze): Comparative evaluation of phenolic composition, antioxidant and antimicrobial activities of seeds cooking water




Physicochemical composition; Fluorescence microscopy; Antimicrobial activity; Residue.


Araucaria angustifolia var. angustifolia and Araucaria angustifolia var. indehiscens, seeds (common pinhão and monkey pinhão) are consumed after cooking, coats and water represent waste. In this work, pinhão was submitted to different cooking conditions, the water extracts were analyzed to determine and identify their phenolic compounds content, antioxidant activity and antimicrobial potential. Cooking for 45 minutes without addition of sodium chloride resulted in residual water with highest content of phenolic compounds and antioxidant activity (DPPH and FRAP), both for the common pinhão and for the monkey pinhão. Protocatecuic acid, vanillin and coniferaldehyde were identified and quantified by HPLC-ESI-MS/MS as the most prevalent phenolic compounds. No antimicrobial potential was observed against Salmonella enterica Typhimurium (ATCC 14028), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) and Bacillus cereus (ATCC 11778).


AOAC International. Official Methods of Analysis. Gaithersburg. (18a ed.).

Arriola, N. A., dos Santos, G. D., Prudêncio, E. S., Vitali, L., Petrus, J. C. C., & Amboni, R. D. M. (2014). Potential of nanofiltration for the concentration of bioactive compounds from watermelon juice. International Journal of Food Science and Technology, 49, 2052–2060.

Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239, 70-76.

Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.

Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Food Science and Technology, 28, 25-30.

Cunha, I. P., Melo, D. W., Verruck, S., Maran, B. M., Prudencio, E. S., & Amante, E. R. (2018). Bioaccessibility of phenolic compounds of Araucaria angustifolia from seed water extracts during in vitro simulated gastrointestinal conditions. Food and Nutrition Sciences, 9, 1137-1146.

Freitas, T. B. de, Santos, C. H. K., Silva, M. V. da, Shirai, M. A., Dias, M. I., Barros, L., Barreiro, M. V., Ferreira, I. C. F. R., Gonçalves, O. H., & Leimann, F. V. (2018). Antioxidants extraction from Pinhão (Araucaria angustifolia (Bertol.) Kuntze) coats and application to zein films. Food Packaging and Shelf Life, 15, 28-34.

D’Archivio, M., Scazzocchio, B., Silenzi, A., Giovannini, C., & Masella, R. (2018). Role of Protocatechuic Acid in Obesity-Related Pathologies: An Update. In: Polyphenols: Mechanisms of Action in Human Health and Disease, pp.181-192. 10.1016/B978-0-12-813006-3.00014-3.

Koehnlein, E. A., Carvajal, A. E. S., Koehnlein, E. M., Coelho-Moreira, J. Das, Inácio, F. D., & Castoldi, R. (2012). Antioxidant Activities and phenolic compounds of raw and cooked Brazilian pinho (Araucaria angustifolia) seeds. African Journal of Food Science, 6, 512-518.

Lima, E.C., Royer, B., Vaghetti, J. C. P., Brasil, J. L., Simon, N. M., & dos Santos, A. A. (2007). Adsorption of Cu(II) on Araucaria angustifolia wastes: determination of the optimal conditions by statistic design of experiments. Journal of Hazardous Materials, 140, 211-220.

Macedo, L. D., Ávila, B. P., Saraiva, J. F. R., Lacerda, M. da S., Domingues, B. P., Pereira, A. M., Guarino, E. de S. G., & Goularte, M. A. (2020). Caracterização química e sensorial de cookies formulados com grãos crioulos e amido de pinhão (Araucaria angustifolia) starch. Research, Society and Development, 9, e3491210677.

National Comittee for Clinical Laboratory Standards. M2-A8: padronização dos testes de sensibilidade a antimicrobianos por disco-difusão: norma aprovada. (8a ed.), Anvisa, 23. Substitui a Norma M2-A7.>.

Pereira, L. P., Amante, E. R., & Ribeiro, D. H. B. (2020). Araucaria angustifolia seeds drying behavior, flour and starch properties. Research, Society and Development, 9, e935986621. v9i8. 6621.

Santos, C. H. K., Baqueta, M. R., Barros, L., Barreiro, M. F., Ferreira, I. C. F. R., Gonçalves, O. H., Bona, E., da Silva, M. V., & Leimann, F. V. (2018). Systematic study on the extraction of antioxidants from pinhão (Araucaria angustifolia (bertol.) kuntze) coat. Food Chemistry, 261, 216-223.

Schulz, M., Borges, G. S. C., Gonzaga, L. V., Seraglio, S. K. T., Olivo, I. S., Azevedo, M. S., Nehring, P., Gois, J. S., Almeida, T. S., Vitali, L, Spudeit, D. A., Micke, G. A., Borges, D. L. G., & Fett, R. (2015). Chemical composition, bioactive compounds and antioxidant capacity of juçara fruit (Euterpe edulis Martius) during ripening. Food Research International, 77, 125-131.

Shanon, E., Jaiswal, A. K., & Abu-Ghannam, N. (2018). Polyphenolic content and antioxidant capacity of white, green, black, and herbal teas: a kinetic study. Food Research, 2, 1 -11.

Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158.

Tanaka, T., Tanaka, T., & Tanaka, M. (2011). Potential cancer chemopreventive activity of protocatechuic acid. Journal of Experimental and Clinical Medicine, 3, 27-33.

Thys, R. C. S., Aires, A. G., Marczak, L. D. F., & Noreña, C. P. Z. (2013). The effect of acid hydrolysis on the technological functional properties of pinhão (Araucaria brasiliensis) starch. Ciência e Tecnologia de Alimentos, 33, 89-94.




How to Cite

CUNHA, I. P. da .; GONÇALVES, E. K. .; NASCIMENTO, L. E. S. .; MELO, D. W. de .; BILUCA, F. C. .; VITALI, L.; COSTA, A. C. de O. .; RIBEIRO, D. H. B. .; AMANTE, E. R. Araucaria angustifolia (bert.) Otto kuntze): Comparative evaluation of phenolic composition, antioxidant and antimicrobial activities of seeds cooking water . Research, Society and Development, [S. l.], v. 10, n. 9, p. e8810917942, 2021. DOI: 10.33448/rsd-v10i9.17942. Disponível em: Acesso em: 26 sep. 2021.



Agrarian and Biological Sciences