Effect of high-power diode laser on radical surfaces exposed as dental tubules obliteration: in vitro study





Laser; Dentin; Diode laser; Dental sensitivity.


The aim of this study was to find adequate parameters of high-power diode laser for obliteration of dental tubules. Newly extracted human teeth (molars) were used for the research that were treated with high-power diode laser and then evaluated by scanning electron microscopy. Roots of 10 healthy teeth were used and prepared in 40 dentin blocks and dividing them into 4 groups: Control Group; G1, G2, G3 (groups treated with high-power diode laser), varying power, energy and application time. The images were evaluated randomly by 2 blinded, calibrated examiners who attributed scores to each image with a level of significance of 5%. The null hypothesis there was no difference between the groups tested regarding of the obliteration of the dentinal tubules. Considering the scores used for analyzing the SEM images, statistically significant differences were observed between the control and all the experimental groups (p < 0,05). However, the irradiated (experimental) groups showed no statistically significant differences between them, because at all the tested parameters the dentin tubules were shown to be obliterated (p < 0.05). The efficacy can be completed in the obliteration of tubules and interruption of fluid movement within the dentin tubules with treatment of the exposed surface with the high-power diode laser; however, the parameters of power and energy lowest showed better results.


Al-Saud, L., & Al-Nahedh, H. (2012). Occluding Effect of Nd:YAG Laser and Different Dentin Desensitizing Agents on Human Dentinal Tubules In Vitro : A Scanning Electron Microscopy Investigation . Operative Dentistry, 37(4), 340–355. https://doi.org/10.2341/10-188-l

Aranha, A. C. C., & De Paula Eduardo, C. (2012). Effects of Er:YAG and Er,Cr:YSGG lasers on dentine hypersensitivity. Short-term clinical evaluation. Lasers in Medical Science, 27(4), 813–818. https://doi.org/10.1007/s10103-011-0988-9

Brännström M. (1966). Sensitivity of dentine. Oral Surg Oral Med Oral Pathol., 4, 517–526.

Chen, M. le, Ding, J. feng, He, Y. jiang, Chen, Y., & Jiang, Q. zhou. (2015). Effect of pretreatment on Er:YAG laser-irradiated dentin. Lasers in Medical Science, 30(2), 753–759. https://doi.org/10.1007/s10103-013-1415-1

Costa, L. M., Cury, M. S., Menezes-Oliveira, M. A. H., Nogueira, R. D., & Geraldo-Martins, V. R. (2016). A utilização da laserterapia para o tratamento da hipersensibilidade dentinária. Journal of Health Sciences, 18(3), 210. https://doi.org/10.17921/2447-8938.2016v18n3p210-6

Cunha, S. R., Garófalo, S. A., Scaramucci, T., Zezell, D. M., & Aranha, A. C. C. (2017). The association between Nd:YAG laser and desensitizing dentifrices for the treatment of dentin hypersensitivity. Lasers in Medical Science, 32(4), 873–880. https://doi.org/10.1007/s10103-017-2187-9

de Araújo, J. G. L., Araújo, E. M. dos S., Rodrigues, F. C. N., Paschoal, M. A. B., & Lago, A. D. N. (2019). High Power Laser and photobiomodulation in oral surgery: Case report. Journal of Lasers in Medical Sciences, 10(1), 75–78. https://doi.org/10.15171/jlms.2019.12

Gholami, G. A., Fekrazad, R., Esmaiel-Nejad, A., & Kalhori, K. A. (2011). An Evaluation of the Occluding Effects of Er;Cr:YSGG, Nd:YAG, CO 2 and Diode Lasers on Dentinal Tubules: A Scanning Electron Microscope In Vitro Study . Photomedicine and Laser Surgery, 29(2), 115–121. https://doi.org/10.1089/pho.2009.2628

Gillam, D. G. (2013). Current diagnosis of dentin hypersensitivity in the dental office: An overview. Clinical Oral Investigations, 17(SUPPL.1), 21–29. https://doi.org/10.1007/s00784-012-0911-1

Glockner, K. (2013). What are the unmet needs in the dental office/at home to treat dentin hypersensitivity? Clinical Oral Investigations, 17(SUPPL.1), 61–62. https://doi.org/10.1007/s00784-012-0914-y

GojkovVukelic, M., Hadzic, S., Zukanovic, A., Pasic, E., & Pavlic, V. (2016). Application of Diode Laser in the Treatment of Dentine Hypersensitivity. Medical Archives, 70(6), 466. https://doi.org/10.5455/medarh.2016.70.466-469

Koche, J. C. (2011). Fundamentos de metodologia científica. Vozes.

K Ozlem, GM Esad, A Ayse, U. A. (2018). Efficiency of Lasers and a Desensitizer Agent on Dentin Hypersensitivity Treatment: A Cinical Study. Original Article, https://doi.org/10.4103/njcp.njcp

Kim, J. won, & Park, J. C. (2017). Dentin hypersensitivity and emerging concepts for treatments. Journal of Oral Biosciences, 59(4), 211–217. https://doi.org/10.1016/j.job.2017.09.001

Leite, M. F. (2017). Hipersensibilidade dentinária : desafios para diagnóstico e perspectivas de tratamento. Rev Assoc Paul Cir Dent, 71(May), 21–24.

Lopes, A. O., de Paula Eduardo, C., & Aranha, A. C. C. (2017). Evaluation of different treatment protocols for dentin hypersensitivity: an 18-month randomized clinical trial. Lasers in Medical Science, 32(5), 1023–1030. https://doi.org/10.1007/s10103-017-2203-0

Mafra, S. C., Girelli, C. F. M., G. Xavier, V. F., Lacerda, M. F. L., Lacerda, G. P., & Coelho, R. G. (2017). A eficácia da solução de EDTA na remoção de smear layer e sua relação com o tempo de uso: uma revisão integrativa. Revista Da Faculdade de Odontologia - UPF, 22(1), 120–129. https://doi.org/10.5335/rfo.v22i1.6305

Moraschini, V. (2018). Effectiveness for dentin hypersensitivity treatment of non-carious cervical lesions : a meta-analysis. 617–631.

Osmari, D., Fraga, S., de Oliveira Ferreira, A. C., de Paula Eduardo, C., Marquezan, M., da Silveira, B. L., Ferreira, A. C. de O., Eduardo, C. de P., & Silveira, B. L. da. (2018). In-office Treatments for Dentin Hypersensitivity: A Randomized Split-mouth Clinical Trial. Oral Health & Preventive Dentistry, 16(2), 125–130. https://doi.org/10.3290/j.ohpd.a40299

Pereira, A. S.; Shitsuka, D. M.; Parreira, F. J.; Shitsuka, R. (2018). Metodologia da pesquisa cientifica. UFSM.

Romano, A. C. C. C., Aranha, A. C. C., Da Silveira, B. L., Baldochi, S. L., & De Paula Eduardo, C. (2011). Evaluation of carbon dioxide laser irradiation associated with calcium hydroxide in the treatment of dentinal hypersensitivity. A preliminary study. Lasers in Medical Science, 26(1), 35–42. https://doi.org/10.1007/s10103-009-0746-4

Saluja, M., Grover, H. S., & Choudhary, P. (2016). Comparative morphologic evaluation and occluding effectiveness of Nd: YAG, CO2 and diode lasers on exposed human dentinal tubules: An invitro SEM Study. Journal of Clinical and Diagnostic Research, 10(7), ZC66–ZC70. https://doi.org/10.7860/JCDR/2016/18262.8188

Schmidlin, P. R., & Sahrmann, P. (2013). Current management of dentin hypersensitivity. 17, 0–4. https://doi.org/10.1007/s00784-012-0912-0

Sgolastra, F., Petrucci, A., Gatto, R., & Monaco, A. (2011). Effectiveness of laser in dentinal hypersensitivity treatment: A systematic review. Journal of Endodontics, 37(3), 297–303. https://doi.org/10.1016/j.joen.2010.11.034

Shiau, H. J. (2012). Dentin hypersensitivity. Journal of Evidence-Based Dental Practice, 12(3 SUPPL.), 220–228. https://doi.org/10.1016/S1532-3382(12)70043-X

Splieth, C. H., & Tachou, A. (2013). Epidemiology of dentin hypersensitivity. Clinical Oral Investigations, 17(SUPPL.1), 3–8. https://doi.org/10.1007/s00784-012-0889-8

van Loveren, C. (2013). Exposed cervical dentin and dentin hypersensitivity summary of the discussion and recommendations. Clinical Oral Investigations, 17(SUPPL.1), 73–76. https://doi.org/10.1007/s00784-012-0902-2

Viana, Í. E. L., Aranha, A. C. C., de Paula Eduardo, C., Farias-Neto, A. M., Machado, A. C., de Freitas, P. M., & Braga, M. M. (2017). Is photobiomodulation (PBM) effective for the treatment of dentin hypersensitivity? A systematic review. Lasers in Medical Science, 33(4), 745–753. https://doi.org/10.1007/s10103-017-2403-7

West, N., Seong, J., & Davies, M. (2012). Dentine hypersensitivity. Erosive Tooth Wear: From Diagnosis to Therapy, 25, 108–122. https://doi.org/10.1159/000360749

West, N. X., Lussi, A., Seong, J., & Hellwig, E. (2013). Dentin hypersensitivity : pain mechanisms and aetiology of exposed cervical dentin. 17, 9–19. https://doi.org/10.1007/s00784-012-0887-x

Yilmaz, H. G., Kurtulmus-Yilmaz, S., Cengiz, E., Bayindir, H., & Aykac, Y. (2011). Clinical evaluation of Er,Cr:YSGG and GaAlAs laser therapy for treating dentine hypersensitivity: A randomized controlled clinical trial. Journal of Dentistry, 39(3), 249–254. https://doi.org/10.1016/j.jdent.2011.01.003

Yu, C. H., & Chang, Y. C. (2014). Clinical efficacy of the Er:YAG laser treatment on hypersensitive dentin. Journal of the Formosan Medical Association, 113(6), 388–391. https://doi.org/10.1016/j.jfma.2013.02.013




How to Cite

MENDES, R. J. S. .; FURTADO, G. S. .; SOUSA, N. M. .; MARQUES, D. M. C. .; DINIZ, R. S. .; GONÇALVES, L. M. .; LAGO, A. D. N. . Effect of high-power diode laser on radical surfaces exposed as dental tubules obliteration: in vitro study. Research, Society and Development, [S. l.], v. 10, n. 9, p. e42410918049, 2021. DOI: 10.33448/rsd-v10i9.18049. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/18049. Acesso em: 18 sep. 2021.



Health Sciences