Histomorphometric analysis of the pectoralis major muscle of elderly and non-elderly autopsied patients with and without AIDS





Muscle cells; Elderly; Acquired immunodeficiency syndrome.


Introduction: The Acquired Immunodeficiency Syndrome (AIDS), an infectious disease, where the main characteristic is the suppression of immunity by T CD4 lymphocytes. Objective: To analyze the percentage of myocytes and collagen fiber density from autopsied fragments of skeletal striated musculature from elderly and non-elderly patients with and without AIDS. Methodology: Retrospective, observational, analytical and quantitative study carried out from 78 divided into four groups, elderly and non-elderly with and without AIDS, aged between 44 and 63 years. Results: For histomorphometric analysis of the percentage of myocytes using Hematoxylin and eosin methods and for collagen fibers and Picrosirius under polarized light, both were performed under ordinary light microscopy. Results: There was a statistically defined decrease in nuclei in the non-elderly groups with and without AIDS and elderly with AIDS compared to the elderly groups without AIDS (p˂0,001). Collagen fibers in the non-elderly and non-deaf AIDS groups were higher when compared to the elderly groups with and without AIDS (p=0,001). The correlation between nucleus and cytoplasm was negative and low in the four groups (p <0,0001), between collagen fibers and nucleus, non-elderly people without AIDS (p<0,0012) and non-elderly people with AIDS (p<0,0368) was negative and decreased, between cytoplasm and collagen fibers the non-elderly group with AIDS (p<0,0353) and non-elderly without AIDS (p<0,0012) was negative and decreased. Conclusion: Aging and AIDS influence the loss of muscle mass and contribute to the reduction of collagen fibers.


Akselrod, S., Gordon, D., Ubel, F. A., Shannon, D. C., Berger, A. C., & Cohen, R. J. (1981). Power spectrum analysis of heart rate fluctuation: a quantitative probe of beat-to-beat cardiovascular control. Science. 213(4504):220-2.

Adigun, R., & Singh R. Tuberculosis. (2021). Oct 27. In: StatPearls [Internet]. Treasure Island (FL): StatPearls.

Avila Rodríguez, M. I., Rodríguez Barroso, L. G., & Sánchez, M. L. (2018). Collagen: A review on its sources and potential cosmetic applications. J Cosmet Dermatol. 17(1):20-26.

Barreto, A. L. S. (2019). Estudo histomorfológico do efeito de membranas de colágeno contendo própolis vermelha sobre o processo de reparo cicatricial por segunda intenção em ratos. Dissertação de mestrado - Universidade Tiradentes.

Bonato, M., Turrini, F., Galli, L., Banfi, G., & Cinque, P. (2020). The Role of Physical Activity for the Management of Sarcopenia in People Living with HIV. Int J Environ Res Public Health. 17(4):1283.

Carnevale, V., Castriotta, V., Piscitelli, P. A., Nieddu, L., Mattera, M., Guglielmi, G., & Scillitani, A. (2018). Assessment of Skeletal Muscle Mass in Older People: Comparison Between 2 Anthropometry-Based Methods and Dual-Energy X-ray Absorptiometry. J Am Med Dir Assoc. 19(9):793-796.

Cavalcante R. O., Cavalcante, A. V. O., Araújo, L. M., Cavalcante, M. C. O., & Vandesmet, L. C. S. (2020). Alterações epiteliais no envelhecimento. Mostra Científica em Biomedicina. 4(2).

Cervi, A., Franceschini, S. C. C., & Priori, S. E. (2005). Análise crítica do uso do índice de massa corporal para idosos. Rev. Nutr. 18(6): 765-775.

Cruz-Jentoft, A.J., Bahat, G., Bauer, J., Boirie, Y., Bruyère, O., Cederholm, T., Cooper, C., Landi, F., Rolland, Y., Sayer, A.A., Schneider, S.M., Sieber, Et Al. C. C., Toponkova, E., Vandewoude, M., Visser, M., & Zamboni. (2019). sarcopenia: revised european consensus on definition and diagnosis. age ageing. 48(1):16-31.

Da Silva, A. A., Gehlen, M. H., Ferreira, C. L. L., & Pereira, A. D. A. (2020). Diagnóstico e intervenções de enfermagem do sistema tegumentar em idosos hospitalizados. South American Journal of Basic Education, Technical and Technological. 7 (2): 351-360.

De Santana, F., Domiciano, D. S., Gonçalves, M. A., Machado, L. G., Figueiredo, C. P., Lopes, J. B., Caparbo, V. F., Takayama, L., Menezes, P. R., & Pereira, R. M. R. (2019). Association of appendicular lean mass, and subcutaneous and visceral adipose tissue with mortality in older brazilians: the são paulo ageing & health study. j. bone miner res. 34 (7): 1264–1274.

Erlandson, K. M., Guaraldi, G., & Falutz, J. (2016). . More than osteoporosis: age-specific issues in bone health. Current Opinion in HIV and AIDS. 3(11): 343–350.

Evans, W. J., Roubenoff, R., & Shevitz, A. (1998). Exercise and the treatment of wasting: aging and human immunodeficiency virus infection. Semin. Encol.25(2- 6):112-22.

Ferreira, F. J. P. A., Cardoso, F. A. G., Pereira, V. G., Nascentes, G. A. N., Monteiro, V. S., & Espindula, A. P. (2021). A síndrome da imunodeficiência humana adquirida como fator na redução da espessura e aumento do colágeno nas trabéculas ósseas de adultos e idosos. Research, Society and Development. 10 (8): 22510817215. DOI: 10.33448/rsd-v10i8.17215. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/17215.

He, N., & Ye, H. (2020). Exercise and Muscle Atrophy. Adv Exp Med Biol. 228:255-267.

Knauth, D,R., Hentges, B., Macedo, J. L., Pilecco, F. B., Teixeira, L. B., & Leal, A. F. (2020). O diagnóstico do HIV/aids em homens heterossexuais: a surpresa permanece mesmo após mais de 30 anos de epidemia.Cadernos de Saúde Pública. 36: e00170118.

Langholz, P. L., Strand, B. H., Cook, S., & Hopstock. (2018). Frailty phenotype and its association with all-cause mortality in community-dwelling Norwegian women and men aged 70 years and older: The Troms Study 2001-2016. Geriatr Gerontol Int. 18(8):1200-1205.

Mccomsey, G. A., Tebas, P., Shane, E., Yin, M. T., Overton, E. T., Huang, J. S., Adrovandi, G. M., Cardoso, S. W., Santana, J. L., & Brown, T. T. (2010). Bone disease in HIV infection: a practical review and recommendations for HIV care providers. Clin Infect Dis. 51 (8):937-946.

Nakagawa, F., Lodwick, R. K., Smith, C. J., Smith, R., Cambiano, V., Lundgren, J. D., Delpech, v., & Phillips, A. N. (2012). Projected life expectancy of people with HIV according to timing of diagnosis. AIDS. 28;26(3):335-43.

Nassr, M., & Nasri, G. (2019). Atrofia muscular esquelética: relação entre ciências básicas e aplicadas (Cinesiologia/Fisioterapia). Fisioterapia e Pesquisa. 26 (1):1-2.

Panwar, P., Butler, G. S., Jamroz, A., Azizi, P., Overall, C. M., & Bromme, D. (2018). Aging-associated modifications of collagen affect its degradation by matrix metalloproteinases. Matrix Biol. 65 (30-44): 28634008.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J & Shitsuka, R. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM.https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1

Pretell-Mazzini, J., Subhawong, T.Y., Hernandez, V. H., & Campo, R. (2016). HIV and orthopaedics: musculoskeletal manifestations and outcomes. J Bone Joint Surg Am. 98(9):775-86.

Sanches-da-Silva, G. N., Medeiros, L. F. S., & Lima, F. M. (2019). The Potential Use of the CRISPR-Cas System for HIV-1 Gene Therapy. Int J Genomics. 2019:14.

Sui, S. X., Williams, L. J., Kew-Holloway, K. L., Hyde, N. K., & Pasco, J. A. (2020). Skeletal Muscle Health and Cognitive Function: A Narrative Review. Int J Mol Sci. 22(1):255.

Sweeney, H. L., & Hammers, D. W. (2018). Muscle Contraction. Cold Spring Harb Perspect Biol. 10(2).

Unaids.(2013). Nações Unidas. GLOBAL REPORT: Unaids report on the global aids epidemic 2013. Genebra, 2013. Disponível em: https://www.unaids.org/en. Acesso em: 20 Mai. 2021.

Yarasheski, K. E., Scherzer, R., Kotler, D. P., Dobs, A. S., Tien, P. C., Lewis, C. E., Kronmal, R. A., Heymsfield, S. B., Bacchetti, P., & Grunfeld, C. (2011). Study of Fat Redistribution and Metabolic Change in HIV Infection (FRAM). Age-related skeletal muscle decline is similar in HIV-infected and uninfected individuals. J Gerontol a Biol Sci Med Sci. 66(3):332-40.

Yilmaz Çankaya, B., Karaman, A., Albez, F.S., Polat, G., Alper, F., & Akgun, M. (2021). The association of silicosis severity with pectoralis major muscle and subcutaneous fat volumes and the pulmonary artery/aorta ratio evaluated by CT. Diagn Interv Radiol. 27(1):37-41.

Zanker, J., & Duque, G. (2019). Osteoporose em pessoas idosas: velhos e novos jogadores. Journal of the American Geriatrics Society. 67(4): 831-840.



How to Cite

PEREIRA , V. G. .; ROCHA, L. A. .; FERREIRA , F. J. P. de A. .; TEIXEIRA, V. de P. A. .; SILVA , A. C. S. da .; ROSA, R. C. .; ESPINDULA, A. P. Histomorphometric analysis of the pectoralis major muscle of elderly and non-elderly autopsied patients with and without AIDS. Research, Society and Development, [S. l.], v. 10, n. 10, p. e151101018516, 2021. DOI: 10.33448/rsd-v10i10.18516. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/18516. Acesso em: 21 oct. 2021.



Health Sciences