Isolation and resistance profile of Candida spp. of domestic and wild animals




Brazil; Candidiasis; Fungus; ITS; Veterinary.


Candida spp. are yeasts present in the microbiota of humans and animals, but due to disorders, they can become pathogenic, causing candidiasis. This study aimed to evaluate the diversity of Candida species in domestic and wild animals and the antifungal susceptibility profile. A total of 79 isolates were obtained from domestic animals (n=49) and wild animals (n=30) collected from 13 different injury sites. The isolates were previously characterized morphologically and tinctorial and the definitive classification occurred by DNA sequencing of the ITS region. The broth microdilution technique obtained the susceptibility profile with the antifungal agents: caspofungin, fluconazole, voriconazole, amphotericin B, nystatin, and itraconazole. Twelve different Candida species were identified, where C. rugosa was the most isolated species (26%, 21/79), followed by C. parapsilosis (20%, 16/79) and C. albicans (15%, 12/79). The susceptibility profile was evaluated in 30 isolates (10 isolates of C. glabrata, ten of C. tropicalis, and ten of C. parapsilosis), resulting in 100% of the tested isolates being resistant to itraconazole. Still, none of them was resistant to voriconazole. Isolates of C. tropicalis were resistant to multiple antifungal agents. These data alerted the worldwide problem of antimicrobial resistance, aggravated by the emergence of strains resistant to multiple drugs. This study is the first study that verified the occurrence and resistance profiles of Candida spp. in animals in Mato Grosso State, Midwest, Brazil, encouraging the execution of new works further to understand the relationship between these yeasts and their hosts.


Araújo, I. M., Lopes, L. P. & Cruz, C. M. (2020). Caracterização sistemática da resposta imune à infecção por Candida. Brazilian Journal of health Review, 3(2), 3788-3803. https://10.34119/bjhrv3n2-203.

Brilhante, R., Rodrigues, T., Castelo-Branco, D., Teixeira, C., Macedo, R. B., Bandeira, S. P., Pereira de Alencar, L., Monteiro, A. J., Cordeiro, R. A., Bandeira, T., Moreira, J., Sidrim, J., & Rocha, M. (2014). Antifungal susceptibility and virulence attributes of animal-derived isolates of Candida parapsilosis complex. Journal of medical microbiology, 63(Pt 11), 1568–1572.

Bumroongthai, K., Chetanachan, P., Niyomtham, W., Yurayart, C., & Prapasarakul, N. (2016). Biofilm production and antifungal susceptibility of co-cultured Malassezia pachydermatis and Candida parapsilosis isolated from canine seborrheic dermatitis. Medical mycology, 54(5), 544–549.

Chakrabarti, A., & Singh, S. (2020). Multidrug-resistant Candida auris: an epidemiological review. Expert review of anti-infective therapy, 18(6), 551–562.

Clinical Laboratory and Standards Institute (CLSI) (2012). Reference method for broth dilution antifungal susceptibility testing yeasts. (Document M27-S4). 4th Edition. Wayne, PA.

Cordeiro, R., Bittencourt, P. V., Brilhante, R. S., Teixeira, C. E., Castelo-Branco, D., Silva, S. T., De Alencar, L. P., Souza, E. R., Bandeira, T., Monteiro, A. J., Sidrim, J. J., & Rocha, M. F. (2013). Species of Candida as a component of the nasal microbiota of healthy horses. Medical mycology, 51(7), 731–736.

Cordeiro, R. A., Sales, J. A., Castelo-Branco, D., Brilhante, R., Ponte, Y. B., Dos Santos Araújo, G., Mendes, P., Pereira, V. S., Alencar, L. P., Pinheiro, A. Q., Sidrim, J., & Rocha, M. (2017). Candida parapsilosis complex in veterinary practice: A historical overview, biology, virulence attributes and antifungal susceptibility traits. Veterinary microbiology, 212, 22–30.

Del Poeta, M., Toffaletti, D. L., Rude, T. H., Dykstra, C. C., Heitman, J., & Perfect, J. R. (1999). Topoisomerase I is essential in Cryptococcus neoformans: role In pathobiology and as an antifungal target. Genetics, 152(1), 167–178.

Denning, D. W., & Hope, W. W. (2010). Therapy for fungal diseases: opportunities and priorities. Trends in microbiology, 18(5), 195–204.

Dudiuk, C., Morales-López, S. E., Podesta, V., Macedo, D., Leonardelli, F., Vitale, R. G., Tosello, M. E., Cabeza, M. S., Biasoli, M., Gamarra, S., & Garcia-Effron, G. (2017). Multiplex PCR designed to differentiate species within the Candida glabrata complex. Revista iberoamericana de micologia, 34(1), 43–45.

Dworecka-Kaszak, B., Biegańska, M. J., & Dąbrowska, I. (2020). Occurrence of various pathogenic and opportunistic fungi in skin diseases of domestic animals: a retrospective study. BMC veterinary research, 16(1), 248.

El-Kholy, M. A., Helaly, G. F., El Ghazzawi, E. F., El-Sawaf, G., & Shawky, S. M. (2021). Virulence Factors and Antifungal Susceptibility Profile of C. tropicalis Isolated from Various Clinical Specimens in Alexandria, Egypt. Journal of fungi (Basel, Switzerland), 7(5), 351.

Garner, C. D., Starr, J. K., McDonough, P. L., & Altier, C. (2010). Molecular identification of veterinary yeast isolates by use of sequence-based analysis of the D1/D2 region of the large ribosomal subunit. Journal of clinical microbiology, 48(6), 2140–2146.

Hernández-Chávez, M. J., Franco, B., Clavijo-Giraldo, D. M., Hernández, N. V., Estrada-Mata, E., & Mora-Montes, H. M. (2018). Role of protein phosphomannosylation in the Candida tropicalis macrophage interaction. FEMS yeast research, 18(5), 10.1093/femsyr/foy053.

Inácio, C. P., Beserra, F. G., Soares, C. R. P., Romaguera, L. M. D., Araújo, P. S. R. de,Buonafina, M. D. S., Araújo Neto, L. N., Santos, D. C. dos e Neves, R.P. (2021). Epidemiologia e padrões de suscetibilidade antifúngica de espécies de Candida em hospitais terciários: Atualização sobre tendências regionais. Research, Society and Development. 10, 4 (abr. 2021), e59810414462.

Kashem, S. W., & Kaplan, D. H. (2016). Skin Immunity to Candida albicans. Trends in immunology, 37(7), 440–450.

Leite-Júnior, D. P., Yamamoto, A. C., Martins, E. R., Teixeira, A. F., & Hahn, R. C. (2011). Species of Candida isolated from anatomically distinct sites in military personnel in Cuiabá, Mato Grosso, Brazil. Anais brasileiros de dermatologia, 86(4), 675–680.

Malek, M., Paluchowska, P., Bogusz, B., & Budak, A. (2017). Molecular characterization of Candida isolates from intensive care unit patients, Krakow, Poland. Revista iberoamericana de micologia, 34(1), 10–16.

Nejad, E., Ghasemi Nejad Almani, P., Mohammadi, M. A., & Salari, S. (2020). Molecular identification of Candida isolates by Real-time PCR-high-resolution melting analysis and investigation of the genetic diversity of Candida species. Journal of clinical laboratory analysis, 34(10), e23444.

Peremalo, T., Madhavan, P., Hamzah, S., Than, L., Wong, E. H., Nasir, M., Chong, P. P., & Ng, K. P. (2019). Antifungal susceptibilities, biofilms, phospholipase and proteinase activities in the Candida rugosa complex and Candida pararugosa isolated from tertiary teaching hospitals. Journal of medical microbiology, 68(3), 346–354.

Pfaller, M. A., Espinel-Ingroff, A., Canton, E., Castanheira, M., Cuenca-Estrella, M., Diekema, D. J., Fothergill, A., Fuller, J., Ghannoum, M., Jones, R. N., Lockhart, S. R., Martin-Mazuelos, E., Melhem, M. S., Ostrosky-Zeichner, L., Pappas, P., Pelaez, T., Peman, J., Rex, J., & Szeszs, M. W. (2012). Wild-type MIC distributions and epidemiological cutoff values for amphotericin B, flucytosine, and itraconazole and Candida spp. as determined by CLSI broth microdilution. Journal of clinical microbiology, 50(6), 2040–2046.

Quinn, P. J., Markey, B. K., Leonard, F. C., Hartigan, P., Fanning, S., & Fitzpatrick, E. S. (2011). Veterinary Microbiology and Microbial Disease. 3 ed. Artmed.

Scaccabarozzi, L., Locatelli, C., Pisoni, G., Manarolla, G., Casula, A., Bronzo, V., & Moroni, P. (2011). Short communication: Epidemiology and genotyping of Candida rugosa strains responsible for persistent intramammary infections in dairy cows. Journal of dairy science, 94(9), 4574–4577.

Sharifzadeh, A., Soltani, M., & Shokri, H. (2015). Evaluation of virulence factors and antifungal susceptibility patterns of different Candida species isolated from the female camel (Camelus dromedarius) genital tract. Mycoses, 58(8), 478–484.

Sidrim, J. J., Carvalho, V. L., de Souza Collares Maia Castelo-Branco, D., Brilhante, R. S., de Melo Guedes, G. M., Barbosa, G. R., Lazzarini, S. M., Oliveira, D. C., de Meirelles, A. C., Attademo, F. L., da Bôaviagem Freire, A. C., de Aquino Pereira-Neto, W., de Aguiar Cordeiro, R., Moreira, J. L., & Rocha, M. F. (2016). Antifungal Resistance and Virulence Among Candida spp. from Captive Amazonian manatees and West Indian Manatees: Potential Impacts on Animal and Environmental Health. EcoHealth, 13(2), 328–338.

Singh, R. I., Xess, I., Mathur, P., Behera, B., Gupta, B., & Misra, M. C. (2011). Epidemiology of candidaemia in critically ill trauma patients: experiences of a level I trauma centre in North India. Journal of medical microbiology, 60(Pt 3), 342–348.

Van de Groep, K., Bos, M. P., Savelkoul, P., Rubenjan, A., Gazenbeek, C., Melchers, W., van der Poll, T., Juffermans, N. P., Ong, D., Bonten, M., Cremer, O. L., & MARS consortium (2018). Development and first evaluation of a novel multiplex real-time PCR on whole blood samples for rapid pathogen identification in critically ill patients with sepsis. European journal of clinical microbiology & infectious diseases: official publication of the European Society of Clinical Microbiology, 37(7), 1333–1344.

Vila, T., Montelongo-Jauregui, D., Ahmed, H., Puthran, T., Sultan, A. S., & Jabra-Rizk, M. A. (2020). Comparative Evaluations of the Pathogenesis of Candida auris Phenotypes and Candida albicans Using Clinically Relevant Murine Models of Infections. mSphere, 5(4), e00760-20.

White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetic. In PCR Protocols: a guide to methods and applications. Academic Press. 315-322.

Yamamoto, A. C., de Paula, C. R., Dias, L. B., Tadano, T., Martins, É. R., Amadio, J. V., & Hahn, R. C. (2012). Epidemiological and clinical characteristics of nosocomial candidiasis in university hospitals in Cuiabá--Mato Grosso, Brazil. Revista iberoamericana de micologia, 29(3), 164–168.

Zhai, L., Zhou, Y., Wu, Y., Jin, Y., Zhu, Q., Gao, S., Li, X., Sun, Z., Xiao, Y., Huang, B., & Tian, K. (2021). Isolation and identification of Candida tropicalis in sows with fatal infection: a case report. BMC veterinary research, 17(1), 108.

Zuza-Alves, D. L., de Medeiros, S. S., de Souza, L. B., Silva-Rocha, W. P., Francisco, E. C., de Araújo, M. C., Lima-Neto, R. G., Neves, R. P., Melo, A. S., & Chaves, G. M. (2016). Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil. Frontiers in microbiology, 7, 1783.



How to Cite

CÂNDIDO, S. L.; MENEZES, I. de G. .; NAKAZATO, L. . .; MORGADO , T. O. .; LUDWIG, A. .; SANTURIO, J. M. .; DUTRA, V. . Isolation and resistance profile of Candida spp. of domestic and wild animals. Research, Society and Development, [S. l.], v. 10, n. 10, p. e264101018541, 2021. DOI: 10.33448/rsd-v10i10.18541. Disponível em: Acesso em: 27 oct. 2021.



Agrarian and Biological Sciences