Development and validation of analytical method by spectrophotometry UV-Vis for quantification of flavonoids in leaves of Senna occidentalis Link
DOI:
https://doi.org/10.33448/rsd-v11i1.18584Keywords:
Senna occidentalis; Total flavonoids; Ultraviolet-Visible spectrophotometry; Validation.Abstract
Senna occidentalis Link (Fabaceae), known as “fedegoso”, is used in folk medicine due to its anti-inflammatory, antioxidant, muscle relaxant and inhibiting lipid peroxidation imputed to flavonoids contained in its composition. The species is also a constituent of processed products are commercialized in various parts of the world including Brazil, although there are few reports in the literature about the development of an analytical method for quantification of flavonoids in it. The main purpose of this research was the evaluation of an analytical methodology to determinate the content of total flavonoids in leaves of S. occidentalis, for quality control. The parameters evaluated were: drug: solvent ratio, concentration of aluminum chloride and reaction time. The quantification of total flavonoids was obtained after reaction with aluminum chloride by UV/Vis spectrophotometry. The results revealed a maximum absorption peak (λ = 392 nm) was the amount of 0.5 g of a plant raw in 100 mL of solvent and there were no significant influences between the concentrations of aluminum chloride or hydroalcoholic solutions analyzed. The evaluation of reaction time allowed to characterize the kinetics as slow, stabilizing from 60 min, choosing this as reading time. The method was specific, linear, precise, accurate and robust, according to the specifications set in RDC n. 166/2017. Finally, the results of the study showed that the measured methodology is simple and accurate and can be applied for quality assessment of the species S. occidentalis.
References
Abubakar, A. R., & Haque, M. (2020). Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. Journal of Pharmacy & Bioallied Science. 12(1), 1–10.
Balekundri, A., & Mannur, V. (2020). Quality control of the traditional herbs and herbal products: A review. Future Journal Of Pharmaceutical Sciences. 6, 67.
Barbosa, E. C. G. S., Viturino, W. A., Machado, J. C. B., Ferreira, M. R. A., & Soares, L. A. L. (2020). Phytochemical evaluation and method validation for quantification of flavonoids in the leaves of Cinnamomum verum. Indo American Journal of Pharmaceutical Sciences. 7, 1419-1419.
Brasil. Agência Nacional de Vigilância Sanitária. 2010a. Resolução RDC nº 14 de 31 de março de 2010; Diário Oficial da União, Brasília, DF, 05 abr. 2010, seção 1 - Agência Nacional de Vigilância Sanitária (ANVISA) dispõe sobre o registro de medicamentos fitoterápicos.
Brasil. Agência Nacional de Vigilância Sanitária. 2010b. Resolução RDC nº 17 de 16 de abril de 2010; Diário Oficial da União, Brasília, DF, 19 abr. 2010, Agência Nacional de Vigilância Sanitária (ANVISA) dispõe sobre as Boas Práticas de Fabricação de Medicamentos.
Brasil, Resolução RDC Nº 166, de 24 de julho de 2017. Dispõe sobre a validação de métodos analíticos e dá outras providências. Anvisa, Ed. Ministério da Saúde/Agência Nacional de Vigilância Sanitária/Diretoria Colegiada: Brasília, 2017.
De Araújo, A. D., Oliveira, F. G. S., Lacerda, F. F., da Silva, C. E. S., dos Santos, B. S., Bezerra-Filho, C. M., Ramos, B. A., Harand, W., da Silva, N. H., Paiva, P. M. G., Almeida, J. R. G. S., Silva, M. V., & Correia, M. T. S. (2021). Phytochemical screening, in vitro antioxidant, photoprotective and themolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu). Research, Society and Development. 10(1), e38610111825.
Gupta, D., & Singh, J. (1991). Flavonoid Glycosides from Cassia alata. Phytochemistry. 30(8), 2761- 2763.
Hatano, T., Mizuta, S., Ito, H., & Yoshida, T. (1999). C-Glycosidic flavonoids from Cassia occidentalis. Phytochemistry. 52, 1379-1383.
Issa, T. O., Ahmed, A. I. M., Mohamed, Y. S., Yagi, S., Makhawi, A. M., & Khider, T. O. (2020). Physiochemical, insecticidal, and antidiabetic activities of Senna occidentalis Linn root. Biochemistry Research International. 15, 8810744.
List PM, Schimidt PC 1989. Phytopharmaceutical technology. Boca Raton: CRC.
Ish, P., Rathi, S., Singh, H., & Anuradha, S. (2019). Senna occidentalis Poisoning: An Uncommon Cause of Liver Failure. ACG Case Reports Journal. 6(4), e00035.
Lozada-Ramírez, J. D., Ortega-Regules, A. E., Hernández, L. R., & de Parrodi, C. A. (2021). Spectroscopic and Spectrometric Applications for the Identification of Bioactive Compounds from Vegetal Extracts. Applied Sciences. 11, 3039.
Luo, C., Zou, X., Li, Y., Sun, C., Jiang, Y., & Wu, Z. (2011). Determination of flavonoids in propolis-rich functional foods by reversed phase high performance liquid chromatography with diode array detection. Food Chemistry. 127, 314-320
Mabry, T. J., Markham, K. R., & Thomas, M. B. (1970). The systematic identification of Flavonoids. Springer: New York.
Matos, L. L., Fernandes, J. M., Ferreira, M. R. A., Langassner, S. M. Z., & Soares, L. A. L. (2016). Avaliação de metodologia analítica espectrofotométrica para quantificação de flavonoides totais nas folhas de Kalanchoe brasiliensis Camb. (Crassulaceae). Boletim Informativo Geum. 7, 1.
Petry, R. D., Ortega, G. G., & Silva, W. B. (2001). Flavonoid content assay: influence of the reagent concentration and reaction time on the spectrophotometric behaviour of the aluminium chloride - flavonoid complex. Die Pharmazie. 56, 465-470.
Ramos, R. T. M., Bezerra, I. C. F., Ferreira, M. R. A., & Soares, L. A. L. (2017). Spectrophotometric quantification of flavonoids in herbal material, crude extract, and fractions from leaves of Eugenia uniflora Linn. Pharmacognosy Research. 9, 253.
Scheidegger, N. M. B., & Rando, J. G. (2020). Cassia in Flora do Brasil 2020. Jardim Botânico do Rio de Janeiro. <http://floradobrasil.jbrj.gov.br/reflora/floradobrasil/FB82817>.
Soares, L. A. L., Bassani, V. L., Ortega, G. G., & Ros Petrovick, P. (2003). Total flavonoid determination for the quality control of aqueous extractives from Phyllanthus niruri L. Latin American Journal of Pharmacy. 22, 203-207.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Vanessa da Silva Luna; Karina Perrelli Randau; Magda Rhayanny Assunção Ferreira; Luiz Alberto Lira Soares
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
