Cronotanatognose, utilização da polpa dentária como ferramenta para determinar o tempo de morte: uma revisão




Post-mortem; Forensic Dentistry; Thanatology.


The post-mortem interval is the period of time that has passed since the death occurred until the moment when the body and /or human remnants are studied. The estimation of this interval is a matter of great relevance in the forensic sphere due to its important role in the resolution of criminal cases. Teeth are fundamental structures in a forensic context due to their high strength and specificity. Therefore, this article aims to present how the dental pulp is used to determine the time of death of the corpse. A systematic review of the scientific literature was carried out with a descriptive and qualitative character, allowing the inclusion of experimental and non-experimental studies for a complete coverage of the analyzed phenomenon. For data collection, online searches were carried out on the bases: SciElo, LILACS and Elsevier. All journal papers that used the selected terms were included in this review. The degradation of dental pulp after death consists of a complex process that has not been fully studied, but with the techniques and changes shown in the text, reliable post-mortem interval results are possible.


Caballín, A., Peréa, B., Agustín, B., Sánchez, J. (2010). Valoración de los cambios histológicos pulpares para la determinación de la data de la muerte. Cient dent.,7, 9-13.

Carrasco, P. A., Brizuela, C. I., Rodriguez, I. A., Muñoz, S., Godoy, M. E., Inostroza, C. (2017). Histological transformations of the dental pulp as possible indicator of post mortem interval: a pilot study. Forensic science international, 279, 251-257.

Catts, V. S., Catts, S. V. (2005). Fernandez, H. R., Taylor, J. M., Coulson, E. J., Lutze-Mann, L. H. A. A microarray study of post-mortem mRNA degradation in mouse brain tissue. Brain Res Mol., 38,164–177.

Conde, M. C., Nedel, F., Campos, V. F., Smith, A.J ., Nör, J. E., Demarco, F. F., Tarquinio, S.B.C. (2012). Odontoblast RNA stability in different temperature-based protocols for tooth storage. Int Endod J., 45, 266-272.

Cohen, S., Hargreaves, K.M. (2011). Pathways of the pulp. 9th editon. New York: Mosby.

Cortês, M. I. S, Bastos, J. V. (2008). Anatomia da cavidade pulpar, In: LMDS Teixeira, P Reher, P (Eds.), Anatomia aplicada à odontologia, 294-314.

Corte-Real, A., Anjos, M. J., Vieira, D. M., Gamero, J. J. (2012). The tooth for molecular analysis and identification: a forensic approach. J Forensic Odontostomatol, 30, 22-28.

Duffy, J. B., Skinner, M. F., Waterfield, J. D. (1991). Rates putrefaction of dental pulp in the Northwest Coast environment. Forensic Sci. Int., 36, 1492- 502.

Ferreira, J. L., Ferreira, A. E. (2008). Ortega, A. I. Methods for the analysis of hard dental tissues exposed to high temperatures. Forensic Sci Int.178, 119–24.

Fordyce, S. L., Kampmann, M. L., Van, D. N. L., Gilbert, M. T. P. (2013). Long-term RNA persistence in post-mortem contexts. Investigative Genetics, 4.

Gaytmenn, R., Sweet, D. (2003). Quantification of forensic DNA from various regions of human teeth. J Forensic Sci., 48, 622-625.

Gomes H. (1980). Medicina Legal. 20a ed. Rio de Janeiro: Editora Freitas Bastos.

Garrido, R. G., Naia, M. J. T. (2014). Cronotanatognose: A influência do clima tropical na determinação do intervalo post-mortem. Lex Humana, 6, 180- 195.

Higgins, D., Rohrlach, A. B., Kaidonis, J., Townsend, G., Austin, J, J. (215) Differential nuclear and mitochondrial DNA preservation in post-mortem teeth with implications for forensic and ancient DNA studies. PloS one, 10, 1-17.

Krishan, K., Kanchan, T., Garg, A. K. (2015). Dental Evidence in Forensic Identification - An Overview, Methodology and Present Status. Open Dentistry Journal, 9, 250–256.

Madeira, C.M. (1997). Anatomia interior do dente. In Anatomia do dente, 99-110.

Mansilla, J., Solis, C., Chávez-Lomeli, M.E., Gama, J.E. (2003). Analysis of colored teeth from Precolumbian tlatelolco: Postmortem transformation or intravitam processes? Am J Phys Anthropol., 120, 73–82.

Malaver, P. C., Yunis, J. J. (2003). Different dental tissues as source of DNA for human identification in forensic cases. Croatian Medical Journal, 44, 306-309.

Menon, L. M. L., Fernandes, M. M., Paranhos, L. R., Da Silva, R. H. A. (2011). Tanatologia forense e odontologia legal: interface e importância na rotina pericial. Odonto., 19, 15-23.

Menon, L. M. L., Prado, K. F. B., Silva, R. H. A. (2011). Avaliação histológica da dentina e do cemento após diferentes tempos de inumação: estudo in vitro. Rsbo, 8, 131–137.

Oliveira, R. N., Silva, S. F. S., Uchoa, D. P., Mesquita, R. A., Nunes, F.D. (2002). Presença de fungos na dentina humana: implicações arqueológicas e forenses. Ciênc Odontol Bras., 7, 87-90.

Poór, V. S., Lukács, D., Nagy, T., Rácz, E., Sipos, K. (2016). The rate of RNA degradation in human dental pulp reveals post-mortem interval. Int J Legal Med, 130, 615-619.

Plourd, C. J. (2010). Science, the Law, and forensic identification. Forensic Dentistry. 2nd ed. New York: CR, 1-9.

Rai, B., Kaur, J., Pink. (2013). Teeth: An Important Aspect for Forensic Science. In: Evidence- Based Forensic Dentistry. Springer, Berlin, Heidelberg, 169-173.

Remualdo, V. R., Nunes, M. H., Melani, R. H., Hirata, M. H., Oliveira, R.N. (2005). Análise do STR- F13A01 e MPSs do mtDNA para fins de identificação humana: comparação de três métodos de extração de DNA de dentes submetidos ao calor. Rev Pós Grad. 12, 437-443.

Sampaio-Silva, F., Magalhães, T., Carvalho, F., Dinis-Oliveira, R. J., Silvestre, R. (2013). Profiling of RNA Degradation for Estimation of Post Mortem Interval. Plos One, 8.

Silva, M. (1997). Compêndio de Odontologia Legal. São Paulo, Medsi Ed. 490.

Silva, R. H. A., Quiezi, R., Bertolacini, C. D. P., Carvalho, S. P. M., Gasque, K. C. S., Almeida, C. T. A., Bicudo, L. A. R. (2012). Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time, RSBO, 9, 67-73.

Silveira, E. M. S. Z. S. F. (2018). Odontologia legal: a importância do DNA para as perícias e peritos. Saúde, Ética & Justiça, 11, 12-18.

Soriano, E. P., de Carvalho, M. V. D., dos Santos, F. B., de Mendoza, C. C., de Araújo, M. D. S. D., Campello, R. I. C. (2009). The post-mortem pink teeth phenomenon: A case report. Med Oral Patol Oral Cir Bucal., 14, 337–339.

Schwartz, T. R., Schwartz, E. Z., Mieszerski, L., Mcnally, L., Kobilinsky, L. (1991). Characterization of deoxyribonucleic acid (DNA) obtained from teeth subjected to various environmental conditions. J Forensic Sci., 36, 979-990.

Szelecz, I., Lösch, S., Seppey, C. V. W., Lara, E., Singer, D., Sorge, F., Tschui, J., Perotti, M. A., Mitchell, E. A. D. (2018). Comparative analysis of bones, mites, soil chemistry, nematodes and soil micro-eukaryotes from a suspected homicide to estimate the post-mortem interval. Sci Rep. 8.

Sweet, D. J., Sweet, C. H. (1995). DNA analysis of dental pulp to link incinerated remains of homicide victim to crime scene. J Forensic Sci., 40, 310-314.

Thapar, R., Choudhry, S., Sinha, A., Bali, R., Shukla, D. (2013). Pink tooth phenomenon: An enigma? Journal of Forensic and Legal Medicine, 20, 912–914.

Tsuchimochi, T., Iwasa, M., Maeno, Y., Koyama, H., Inoue, H., Isobe, I., Matoba, R., Yokoi, M., Nagao, M. (2002). Chelating resin-based extraction of DNA from dental pulp and sex determination from incinerated teeth with Y-chromosomal alphoid repeat and short tandem repeats. Am J Forensic Med Pathol., 23, 268-271.

Vavpotic, M., Turk, T., Martincic, D.S., Balazic, J. (2009). Characteristics of the number of odontoblasts in human dental pulp post-mortem. Forensic Sci Int., 193, 122–126.

Vass, A. A., Barshick, S. A., Sega, G., Caton, J., Skeen, J. T., Love, J.C., Synstelien, J. A. (2002). Decomposition chemistry of human remains: a new methodology for determining the postmortem interval. Journal of Forensic Science, 47, 542-553.

Vieira, G., Tavares, C. A. P., Bouchardet, F. C. H. (2010). Análise de DNA em Odontologia Forense. Arq Bras Odontol., 6, 57-63.

Young, S. T., Jeffrey, D. W., Hobbs, G.R., Bishop, C.P. (2013). Estimating Postmortem interval using RNA degradation and morphological changes in tooth pulp. Forensic Science International, 229, 163: e1–163.e6.




How to Cite

RAMOS, G. F. .; FERREIRA, E. M. S. .; SILVA, J. F. M. da .; PIMENTA, R. S. Cronotanatognose, utilização da polpa dentária como ferramenta para determinar o tempo de morte: uma revisão. Research, Society and Development, [S. l.], v. 10, n. 10, p. e353101018862, 2021. DOI: 10.33448/rsd-v10i10.18862. Disponível em: Acesso em: 24 feb. 2024.



Review Article