Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)
DOI:
https://doi.org/10.33448/rsd-v10i12.20378Keywords:
Alkaloids; Plant biotechnology; In vitro cultivation.Abstract
Crinum americanum L. is a monocotyledonous plant belonging to the Amaryllidaceae family that has economic importance for being ornamental and pharmacological for containg alkaloids that can present anticholinesterase action in its chemical composition. Callus induction is important both for biomass production and for the accumulation of secondary metabolites, especially those of the alkaloid class. In this sense, biotechnological techniques can be used as an alternative to make the process viable. Nowadays, it is known that monocotyledonous plants present great difficulties to be cultivated in vitro, among which the species C. americanum stands out. As a result, the objective this study was to demonstrate the most efficient concentrations of growth regulators 2,4-D and BAP associated with solidifying agents Agar and Phytagel for in vitro cultivation of this species in order to present alternatives to increase production of biomass and help in future studies in the area of somatic embryogenesis and/or for accumulation of secondary metabolites. For the evaluation of cell development, growth in diameter was observed and the callus growth curve was also obtained through sacrificial sampling. It was observed that when the friable callus is inoculated with the correct balance between growth regulators in MS medium supplemented with 3% sucrose (m/v), which contains the solidifying agent Phytagel at 0.2% (m/v), cells tend to grow faster and consequently the gain in biomass is greater.
References
Alcantara G. B. et al. (2017). Efeito de diferentes antioxidantes e luminosidade no controle da oxidação e multiplicação in vitro de genótipos da cana-de-açúcar. Revista Spacios, 47 (38), 22.
Almeida. G. M & Rodrigues, J. G. L. (2016). Desenvolvimento de plantas através da interferência de auxinas, citocininas, etileno e giberelinas. Brazilian Journal of Applied Technology for agricultural Science. 3 (9), 111-117.
Araújo, S. F. et al. (2018). Biotecnologia Vegetal: Indução de calos da espécie Crinum americanum L. (Amaryllidaceae). In: Santos, S.; Lucas, F. C. A.; Junior, M. R. M.; Santos, A. S. (Org.) Bioculturalidade, Conservação e Biotecnologia na Amazônia Oriental. pp (375-389). Curitiba, PR: Editora CRV.
Berkov, S. et al. (2012). GC-MS of Amaryllidace ousgalanthamine-type-alkaloids. Journal of Mass Spectrometry, 47 (8), 1065-1073.
Berrios, E. F., L. et al.(1999). Influence of genotype and gelling agents on in vitro regeneration by organogenesis in sunflower. Plant Cell, Tissue and Organ Culture. (59), 65-69.
Beruto, M. P et al. (1999). Influence of agar on in vitro cultures: I Physicochemical properties of agar and agar gelled media. In vitro Cellular & Developmental Biology - Plant, (35), p.86-93.
Bornman, C. H. & Vogelmann, T. C. (1984). Effect of rigidity of gel medium on benzyladenine induced adventitious bud formation and vitrification in vitro in Picea abies. Physiologia Plantarum, (61), 505-512.
Cordeiro, I. M. C. C., Lameira, O. A., Ohashi, S. T. & Rosal, L. F. (2004). Efeito de BAP sobre a proliferação de brotos in vitro de Schizolobium amazonicum (paricá). Cerne, 1 (10), 118-124.
Corpes, R. S, et al. (2019). Comparison of Volatile Profile and Antioxidant Activity of Piper divaricatum G. Meyer (Piperaceae) Using Cuttings and Cell Tissue. Journal of the Brazilian Chemical Society, n.11 (30), 2291-2298.
Corpes. R. S, et al. (2020). Germinação in vitro, formação de plântulas e Produção de calos de Crinum americanum L. (Amaryllidaceae). Uma alternativa para produção de metabólitos secundários. In: Silva-Matos; R. R. S.; Oliveira, A. R. F.; Albano-Machado, F. G. (Org.) Floricultura, plantas ornamentais e cultura de tecidos de plantas. pp (12-23) Ponta Grossa, PR: Atena Editora.
Costa, A. S. (2006). Sustentabilidade da Produção de Alecrim pimenta (Lippia sidoides Cham.): Micropropagação visando à conservação in vitro. 70 f. Dissertação (Mestrado), Universidade Federal de Sergipe, SE.
Fagundes. E. (2020). Fitorreguladores no crescimento, produtividade, aspectos fisiológicos e nutricionais em videiras Vitis vinífera. 124 f. Tese (Doutorado) Universidade do Estado de Santa Catarina, SC.
Jardim. L. S. et al. (2010). Efeito de diferentes reguladores de crescimento na regeneração in vitro de pau-rosa (Aniba rosaeodora Ducke). Acta Amazonica, 2 (40) 275-280.
Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassys with tobacco tissue cultures. Physiologia Plantarum, (15), 473-479.
Oliveira. K. S et al.(2019). Influência de reguladores de crescimento e do tipo de explante na morfogênese in vitro de Hancornia speciosa Gomes. Revista Desafios, 4 (6).
Paiva, R. & Paiva, P. D. O. (2001). Cultura de tecidos, Lavras: UFLA/FAEPA.
Ribeiro J. P. N et al. (2009). Efeitos alelopáticos de extratos aquosos de Crinum americanum L. Revista Brasileira de Botânica, 1 (32), 183-188.
Ribeiro, A. P. O. (2006). Influência do genótipo, agentes gelificantes, precursor (ACC) e inibidores (AVG e STS) do etileno e tipo de vedação na morfogênese in vitro de berinjela (Solanum melongena L.). 127 f. Tese (Doutorado) – Universidade Federal de Viçosa, MG.
Scholten, H. J. & Pierik R. L. M. (1998a). Ágar as gelling agent: diferential biological effects in vitro. Scientia Horticulturae. (77), 109-116.
Singh, M. B. & Bhalla, P.L. (2006). Plant stem cells carve their own niche. Trends in Plant Scienc, (11), 241-246.
Soares, G. de A. (2003). Aspectos do cultivo in vitro do ingazeiro [Inga Vera Willd. subsp. affinis (DC) T. D. Penn]. 90 f. Dissertação (Mestrado) -
Universidade Federal de Lavras, MG.
Yew, C. K., et al. (2010). The effect of cytokinins on in vitro shoot length and multiplication of Hymenocallis littoralis. Journal of Medicinal Plants Research, 24 (4), 2641-2646.
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