Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus

Authors

DOI:

https://doi.org/10.33448/rsd-v10i12.20493

Keywords:

Anti-helminthic; Buriti; Cerrado; Gas chromatography; Gastrointestinal nematodes; Sheep.

Abstract

In this study we evaluated the potential of Mauritia flexuosa leaves in the egg hatching and larval development inhibition and for reduction of egg count of this nematode in sheep feces. The leaves of this palm were collected and dehydrated for the production of aqueous and ethanolic extracts with and without tannins. Gas chromatography analysis indicated the presence of fifteen and ten major compounds in the aqueous and ethanolic extracts, respectively, and both showed catechin peaks. The total of condensed tannins for leaves of Mauritia flexuosa was 33.23% ± 2. Aqueous and ethanolic extracts showed 100% anthelmintic activity to inhibit hatchability at 75 mg/ml. The inhibitory concentrations LC90 for aqueous and ethanolic extracts were, respectively, 21.8 and 8.5 mg/ml. The dehydrated leaves powder of M. flexuosa at ≥ 152.08 mg / g of coproculture presented efficiencies greater than 80% for inhibition of larval development. The in vivo administration of aqueous extract at 62.1 mg/kg PC promoted anti-helminthic efficacy of 54.57% and, after 14 days, no clinical signs of toxicity and clinical changes were observed in the treated lambs, indicating potential of this extract for the control alternative of haemonchosis.

References

Bizimenyera, E. S., Githiori, J. B, Eloff, J. N. & Swan, G. E. (2006). In vitro activity of Peltophorum africanum Sond. (Fabaceae) extracts on the egg hatching and larval development of the parasitic nematode Trichostrongylus colubriformis. Veterinary Parasitolology 142, 336-343.

Borges, C. C. L. (2003). Atividade in vitro de anti-helmínticos sobre larvas infectantes de nematódeos gastrintestinais de caprinos, utilizando a técnica de coprocultura quantitativa (Ueno, 1995). Parasitolology Latin American 58, 142 -147.

Coles, G. C, Bauer, C., Borgsteede, F. H., Geerts, S., Klei, T. R., Taylor, M. A. & Waller, P. J. (1992). World Association for the Advancement of Veterinary Parasitology (WAAVP) - Methods for detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitolology 44, 35–44.

Duarte, E. R., Silva, R. B., Vasconcelos, V. O., Nogueira, F. A., & Oliveira, N. J. F. (2012). Diagnóstico do controle e perfil de sensibilidade de nematoides de ovinos ao albendazol e ao levamisol no norte de Minas Gerais. Pesquisa Veterinária Brasileira, 32, 147-152.

Ferreira, I. M. (2005). Bioma Cerrado: caracterização do subsistema de vereda. In: ENCONTRO REGIONAL DE GEOGRAFIA: Novas territorialidades, integração e redefinição regional, 14, 2005, Porto Nacional, Anais.

Gordon, H. M. & Whitlock, H. V. (1939). A new technique for counting nematode eggs in sheep faeces. Journal of the Council for Scientific and Industrial Research 12, 50–52.

Hiermann, A., Kartnig, T.H., & Azzam, S. (1986). Ein beitrag zur quantitativen bestimmung der procyanidine in crataegus. Scientia Pharmaceutica 54, 331–337.

Jackson, F., Varady, M., & Bartley, D. J. (2012). Managing anthelmintic resistance in goats: can we learn lesson from sheep? Small Ruminant Research 103, 3-9.

Klongsiriwet, C., Quijada, J., Williams, A. R., Mueller-Harvey, I., Williamson, E. M., & Hoste, H. (2015). Synergistic inhibition of Haemonchus contortus exsheathment by flavonoid monomers and condensed tannins. International Journal for Parasitology Drugs and Drug Resistance 5, 127–134.

Learmount, J., Sthephens, N., Boughtflower, V. & Barrecheguren, A. (2016). The development of anthelmintic resistance with best practice control of nematodes on commercial sheep farms in the UK. Veterinary Parasitolology 229, 9–14.

Morais-Costa, F., Bastos, G.A., Soares, A. C. M., Costa, E. G. L., Vasconcelos, V. O., Oliveira, N. J. F., Braga, F. C., Duarte, E. R. & Lima, W. S. (2016). In vitro and in vivo action of Piptadenia viridiflora (Kunth) Benth against Haemonchus contortus in sheep. Veterinary Parasitolology 223, 43-49.

Nery, P. S, Nogueira, F. A, Martins, E. R. & Duarte, E. R. (2010). Effect of Anacardium humile on the larval development of gastrintestinal nematodes of sheep. Veterinary Parasitolology 171, 361–364.

Nyman, U., Joshi, P., Madsen, L. B., Pinstrup, M., Rajasekharan, S., George, V. & Pushpangadan, P. (1998). Ethnomedical information and in vitro screening forangiotensin-converting enzyme inhibition of plants utilized as traditionalmedicines in Gujarat, Rajasthan and Kerala (India). Journal of Ethnopharmacology 60, 247–263.

Oliveira, L. M. B., Bevilaqua, C. M. L., Costa, C. T. C, Macedo, I. T. F, Barros, R. S., Rodrigues, A. C. M., Camurça-Vasconcelos, A. L. F., Morais, S. M., Lima, Y. C., Vieira, L. S., & Navarro, A. M. C. (2009). Anthelmintic activity of Cocos nucifera L. against sheep gastrointestinal nematodes. Veterinary Parasitolology 159, 55-59.

Paniagua-Zambrana, N., Cámara-Leret, R. & Macía, M. J. (2015). Patterns of Medicinal Use of Palms Across Northwestern South America. The Botanic Review 81, 317.

Ueno, H. & Gonçalves, P. C. 1998. Manual para diagnóstico das helmintoses de ruminantes. Japan International Cooperation Agency.

Vieira, V. D., Feitosa, T. F., Vilela, V. L. R, Azevedo, S. S., Almeida Neto, J. L., Morais, D. F., Ribeiro, A. R. C. & Athayde, A. C. R. (2014). Prevalence and risk factors associated with goat gastrointestinal helminthiasis in the Sertão region of Paraíba State, Brazil. Tropical Animal Health Producion 46, 355-361.

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Published

20/09/2021

How to Cite

INÁCIO, M. F. .; MORAIS-COSTA, F.; CARDOSO FILHO, O. .; MARTINS, I. P. .; MARTINS JÚNIOR, V. S. .; DUARTE, E. R. .; ARRUDAS, S. R. .; NUNES, Y. R. F. . Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus . Research, Society and Development, [S. l.], v. 10, n. 12, p. e276101220493, 2021. DOI: 10.33448/rsd-v10i12.20493. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/20493. Acesso em: 19 apr. 2024.

Issue

Vol. 10 No. 12

Section

Agrarian and Biological Sciences

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Copyright (c) 2021 Matheus Ferreira Inácio; Franciellen Morais-Costa; Otávio Cardoso Filho; Isabela Parolis Martins; Valdo Soares Martins Júnior; Eduardo Robson Duarte; Sônia Ribeiro Arrudas; Yule Roberta Ferreira Nunes

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