Alcoholic extract of propolis for worms control in sheep
DOI:
https://doi.org/10.33448/rsd-v11i1.20617Keywords:
Antiparasitic; Food safety; Sanitation; Santa Inês; Worms.Abstract
The objective was to evaluate the antiparasitic effect of different dosages of the alcoholic extract of propolis in the control of verminosis in sheep. Thirty-two Santa Inês sheep were used in a randomized complete block design with four treatments and eight replicates. The treatments were: 10 mL of water; 10 mL of alcohol 70%; 5 mL of propolis alcoholic extract (PAE) 30%; and 10 mL of PAE 30%. The treatments were administered orally, in a single dose. The effects of the treatments were evaluated by counting eggs per gram of faeces (EPG) for days 1; 3; 7 and 21 after administration. Oral administration of 10 mL of water or 10 mL of alcohol 70% had no effect on EPG reduction. The use of 5 mL of PAE 30% reduced EPG on days 3 and 7, reducing its efficacy on day 21, while the use of 10 mL of PAE 30% reduced EPG on all evaluated days. Thus, oral administration, in a single dose, of 10 mL of propolis alcoholic extract 30%, effectively reduced the number of EPG demonstrating antiparasitic effect and can be used in the control of verminoses as an alternative method to chemical products synthetic.
References
Buck, E. L., Mizubuti, I. Y., Souza, F. P., Ribeiro, R. P., Filho, A. L., Bracchini, G., & Lopera-Barrero, N. M. (2017). Effect of propolis extract on the parasite load of Nile tilapias reared in cages. Semina: Ciências Agrárias, 38 (3), 1615-1623. https://doi.org/10.5433/1679-0359.2017v38n3p1615
Calegari, M. A., Prasniewski, A., Silva, C. D., Sado, R. Y., Maia, F., Tonial, L., & Oldoni, T. L. (2017). Propolis from Southwest of Parana produced by selected bees: Influence of seasonality and food supplementation on antioxidant activity and phenolic profile. Anais da Academia Brasileira de Ciências, 89 (1), 45-55. https://doi.org/10.1590/0001-3765201620160499
Coelho, J., Falcao, S. I., Vale, N., Almeida-Muradian, L. B., & Vilas-Boas, M. (2017). Phenolic composition and antioxidant activity assessment of southeastern and south Brazilian propolis. Journal of Apicultural Research, 56(1), 21-31. https://doi.org/10.1080/00218839.2016.1277602
Cruz-Cervantes, J. A., Benavides-González, F., Sánchez-Martínez, J. G., Vázquez-Sauceda, M. D. L. L., & Ruiz-Uribe, A. J. (2018). Propolis in aquaculture: A review of its potential. Reviews in Fisheries Science & Aquaculture, 26(3), 337-349. https://doi.org/10.1080/23308249.2018.1424798
Ferreira, F. B. P., Pereira, M. F., Viana, R. V., Ferarrese, L., Cerquetani, J. D. A., Alberton, O., & Gazim, Z. C. (2013). An in vitro assessment of the alcoholic extract in propolis for the control of Rhipicephalus (Boophilus) microplus. Arquivos de Ciências Veterinárias e Zoologia da UNIPAR, 16(2), 107-112.
Garcia, R. C., de Sá, M. E. P., Langoni, H., & Funari, S. R. C. (2004). Effect of alcohol extract of propolis on Pasteurella multocida in vitro and in rabbits. Acta Scientiarum-Animal Sciences, 26(1), 69-77.
Gharsa, H., Slama, K. B., Lozano, C., Gómez-Sanz, E., Klibi, N., Sallem, R. B., & Torres, C. (2012). Prevalence, antibiotic resistance, virulence traits and genetic lineages of Staphylococcus aureus in healthy sheep in Tunisia. Veterinary Microbiology, 156(3-4), 367-373. https://doi.org/10.1016/j.vetmic.2011.11.009
Goncuoglu, M., Ormanci, F. S. B., Ayaz, N. D., & Erol, I. (2010). Antibiotic resistance of Escherichia coli O157: H7 isolated from cattle and sheep. Annals of Microbiology, 60(3), 489-494. https://doi.org/10.1007/s13213-010-0074-8.
Gordon, H. M., & Whitlock, H. V. (1939). A new technique for counting nematode eggs in sheep faeces. Journal of the Council for Scientific and Industrial Research, 12(1), 50-52.
Hegazi, A. G., Abd El Hady, F. K., & Shalaby, H. A. (2007). An in vitro effect of propolis on adult worms of Fasciola gigantica. Veterinary Parasitology, 144(3-4), 279-286. https://doi.org/10.1016/j.vetpar.2006.10.006
Heinzen, E. L., Peixoto, E. D. M., Jardim, J. G., Garcia, R. C., Oliveira, N. T. E., & Orsi, R. D. O. (2012). Extract of propolis in the control of helminthiasis in calves. Acta Veterinaria Brasilica, 6(1), 40-44.
IBGE. (2018). Produção da Pecuaria Municipal. Instituto Brasileiro de Geografia e Estatistica, v.44.
Issa, R.M. (2015). Schistosoma mansoni: The prophylactic and curative effects of propolis in experimentally infected mice. Rawal Medical Journal, 32, 94–98.
Ítavo, C. C. B. F., Ítavo, L. C. V., Esteves, C. A. T., Sapaterro, G. A., Silva, J. A., Silva, P. C. G., & Arco, T. F. F. S. (2019). Influence of solid residue from alcoholic extraction of brown propolis on intake, digestibility, performance, carcass and meat characteristics of lambs in feedlot. Journal Animal and Feed Sciences, 28, 149-158.
Kenyon, F., Hutchings, F., Morgan-Davies, C., van Dijk, J., & Bartley, D. J. (2017). Worm control in livestock: bringing science to the field. Trends in parasitology, 33(9), 669-677. https://doi.org/10.1016/j.pt.2017.05.008
Lima, M., Orsi, R. D. O., Costa, G. D. M., & Malaspina, O. (2015). Brazilian propolis production by africanized bees (Apis mellifera). Bee World, 92(3), 58-68. https://doi.org/10.1080/0005772X.2015.1129229
Machado, B., Pulcino, T. N., Silva, A. L., Tadeu, D., Melo, R. G. S., & Mendonça, I. G. (2016). Propolis as an alternative in prevention and control of dental cavity. Journal Apither, 1(2), 47-50. https://doi.org/10.5455/ja.20160726115117
Monroy, Y. M., Rodrigues, R. A., Rodrigues, M. V., & Cabral, F. A. (2018). Fractionation of ethanolic and hydroalcoholic extracts of green propolis using supercritical carbon dioxide as an anti-solvent to obtain artepillin rich-extract. The Journal of Supercritical Fluids, 138, 167-173. https://doi.org/10.1016/j.supflu.2018.04.016
Morsy, A. S., Soltan, Y. A., Sallam, S. M., Alencar, S. M., & Abdalla, A. L. (2016). Impact of Brazilian red propolis extract on blood metabolites, milk production, and lamb performance of Santa Inês ewes. Tropical Animal Health and Production, 48(5), 1043-1050.
Morsy, A. S., Abdalla, A. L., Soltan, Y. A., Sallam, S. M., El-Azrak, K. E. D. M., Louvandini, H., & Alencar, S. M. (2013). Effect of Brazilian red propolis administration on hematological, biochemical variables and parasitic response of Santa Inês ewes during and after flushing period. Tropical Animal Health and Production, 45(7), 1609-1618. https://doi.org/10.1007/s11250-013-0406-3
Principal, J., Hernández, I., D’aubeterre, R. Gervacio R. J. (2002). Efficacy of propolis on the control of naturally infested sheep helminthiasis. Revista Cientifica, 12(2), 604–607.
Salkova, D. S., Panayotova–Pencheva, M. S., Movsesyan, S. O. (2015). Some bee products as antiparasitic remedies. Russian Journal of Parasitology, 619, 75–78.
Santos, H. C. D., Vieira, D. S., Yamamoto, S. M., Costa, M. M., Sá, M. C., Silva, E., & Silva, T. (2019). Antimicrobial activity of propolis extract fractions against Staphylococcus spp. isolated from goat mastitis. Pesquisa Veterinária Brasileira, 39(12) 954-960. https://doi.org/10.1590/1678-5150-PVB-5940
Sargison, N. D. (2020). The critical importance of planned small ruminant livestock health and production in addressing global challenges surrounding food production and poverty alleviation. New Zealand Veterinary Journal, 68(3), 136-144. https://doi.org/10.1080/00480169.2020.1719373
Sena-Lopes, A., Bezerra, F. S. B., das Neves, R. N., Pinho, R. B., Silva, M. T. D. O., Savegnago, L., & Borsuk, S. (2018). Chemical composition, immunostimulatory, cytotoxic and antiparasitic activities of the essential oil from Brazilian red propolis. PLoS One, 13(2), e0191797. https://doi.org/10.1371/journal.pone.0191797
Shabana, I. I., & Al-Enazi, A. T. (2020). Investigation of plasmid-mediated resistance in E. coli isolated from healthy and diarrheic sheep and goats. Saudi Journal of Biological Sciences, 27(3), 788-796. https://doi.org/10.1016/j.sjbs.2020.01.009
Shedeed, H. A., Farrag, B., Elwakeel, E. A., Abd El-Hamid, I. S., & El-Rayes, M. A. H. (2019). Propolis supplementation improved productivity, oxidative status, and immune response of Barki ewes and lambs. Veterinary World, 12(6), 834. https://doi.org/10.14202/vetworld.2019.834-843
Silva, C. S. R., Barreto, C. L. P., Peixoto, R. M., Mota, R. A., Ribeiro, M. F., & Costa, M. M. (2012). Antibacterial effect of Brazilian brown propolis in different solvents against staphylococcus spp. Isolated from caprine mastitis. Ciência Animal Brasileira, 13(2), 247-251.
Slanzon, G. S., Toledo, A. F., Silva, A. P., Coelho, M. G., da Silva, M. D., Cezar, A. M., & Bittar, C. M. M. (2019). Red propolis as an additive for preweaned dairy calves: Effect on growth performance, health, and selected blood parameters. Journal of Dairy Science, 102(10), 8952-8962.
Snedecor, G.W., & Cochran, W. G. (1989). Statistical methods. 8.ed. Lowa: State Univ. Press Iowa.
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