Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma




Oral squamous cell carcinoma, stem cells, Nestin, Nanog; Oral squamous cell carcinoma; Stem cells; Nestin; Nanog.


Oral carcinogenesis is a highly complex process. Molecular studies demonstrate that some oral cancers progress from pre-neoplastic lesions. Cancer stem cells (CSCs), a small cellular group within the tumor, have currently received much attention by its self-renewal and tumor differentiation ability. It is considered that CSCs may be responsible for the relapse and metastasis of cancer. CSCs have been reported to express common embryonic stem cells markers such as Nestin and Nanog. The aim of the present study was to identify the presence of Nestin and Nanog markers in patients with oral squamous cell carcinoma (OSCC). Nine samples were collected from patients diagnosed with OSCC and two healthy oral mucosa were used as control. The markers expression in nine OSCC was determined by end-point PCR. The OSCC population analyzed was mostly composed of black and white males, with a mean age of 70.6 years, that reported alcohol and tobacco consumption. Nestin and Nanog stem cell markers were expressed in all OSCCs and normal samples analyzed. As these two markers have already been related to the mechanism of metastasis and relapses in cancer, its expression determination could help to understand the aggressive and invasive nature of OSCC.


Celetti, A., Merolla, F., Luise, C., Siano, M., Staibano, S. (2012) Novel Markers for Diagnosis and Prognosis of Oral Intraepithelial Neoplasia. INTECH Open Access Publisher.

Clevers, H. (2011) The cancer stem cell: premises, promises and challenges. Nat Med.; 17(3): 313-9.

Chiou, S. H., Yu, C. C., Huang, C. Y., Lin, S. C., Liu, C. J., Tsai, T. H., Chou, S. H., Chien, C. S., Ku, H. H., Lo, J. F. (2008) Positive correlations of OCT4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma. Clin Cancer Res.; 14(13): 4085-95.

Chiou, S. H., Wang, M. L., Chou, Y. T., Chen, C. J., Hong, C. F., Hsieh, W. J., Chang, H. T., Chen, Y. S., Lin, T. W., Hsu, H. S., Wu, C. W. (2010) Coexpression of Oct4 and Nanog enhances malignancy in lung adenocarcinoma by inducing cancer stem cell-like properties and epithelial-mesenchymal transdifferentiation. Cancer Res.; 70(24): 10433-44.

Silva, S. D., Hier, M., Mlynarek, A., Kowalski, L. P., Alaoui-Jamali, M. A. (2012) Recurrent oral cancer: current and emerging therapeutic approaches. Front Pharmacol.; 3: 1-7.

Dean, M., Fojo, T., Bates, S. (2005) Tumour stem cells and drug resistance. Nat Rev Cancer.; 5(4): 275-84.

Dong, R., Liu, X., Fan, M., Yang, L., Peng, L., Zhang, L. (2010) Isolation and differentiation of nestin positive cells from rat oral mucosal lamina propria. Differentiation.; 79(1): 9-14.

Evander, M., Frazer, I. H., Payne, E., Qi, Y. M., Hengst, K., McMillan, N. A. (1997) Identification of the alpha6 integrin as a candidate receptor for papillomaviruses. J Virol.; 71(3): 2449-56.

Facompre, N., Nakagawa, H., Herlyn, M., Basu, D. (2012) Stem-like cells and therapy resistance in squamous cell carcinomas. Adv Pharmacol.; 65: 235-65.

Kaur, P., Li, A. (2000) Adhesive properties of human basal epidermal cells: an analysis of keratinocyte stem cells, transit amplifying cells, and postmitotic differentiating cells. J Invest Dermatol.; 114(3): 413-20.

Lippman, S. M, Sudbø, J., Hong, W. K. (2005). Oral cancer prevention and the evolution of molecular-targeted drug development. Oral cancer prevention and the evolution of molecular-targeted drug development. J Clin Oncol.; 23(2): 346-56.

Marynka-Kalmani, K., Treves, S., Yafee, M., Rachima, H., Gafni, Y., Cohen, M. A., Pitaru, S. (2010) The lamina propria of adult human oral mucosa harbors a novel stem cell population. Stem Cells.; 28(5): 984-95.

Mehanna, H., Paleri, V., West, C. M., Nutting, C. (2010) Head and neck cancer-Part 1: Epidemiology, presentation, and prevention. BMJ.; 341: 4684.

Mitsui, K., Tokuzawa, Y., Itoh, H., Segawa, K., Murakami, M., Takahashi, K., Maruyama, M., Maeda, M., Yamanaka, S. (2003) The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell.; 113(5): 631-42.

Mulhall, H. J., Labeed, F. H., Kazmi, B., Costea, D. E., Hughes, M. P., Lewis, M. P. (2011) Cancer, pre-cancer and normal oral cells distinguished by dielectrophoresis. Anal Bioanal Chem.; 401(8): 2455-63.

Owens, D. M., Watt, F. M. (2003) Contribution of stem cells and differentiated cells to epidermal tumours. Nat Rev Cancer.; 3(6): 444-51.

Palla, A. R., Piazzolla, D., Alcazar, N., Cañamero, M., Graña, O., Gómez-López, G., Dominguez, O., Dueñas, M., Paramio, J. M., Serrano, M. (2015) The pluripotency factor NANOG promotes the formation of squamous cell carcinomas. Sci Rep.; 5: 10205.

Smane-Filipova, L., Pilmane, M., Akota I. (2016) Immunohistochemical analysis of nestin, CD34 and TGFβ3 in facial tissue of children with complete unilateral and bilateral cleft lip and palate. Stomatologija.; 18(3): 98-104.

Wiese, C., Rolletschek, A., Kania, G., Blyszczuk, P., Tarasov, K. V., Tarasova, Y., Wersto, R. P., Boheler, K. R., Wobus, A. M. (2004) Nestin expression--a property of multi-lineage progenitor cells? Cell Mol Life Sci.; 61(19-20): 2510-22.




How to Cite

CADILHO, J. C. R. .; LYRA, S. M. de C.; LIMA, A. B. M.; BOGHOSSIAN, C. M. da S.; PEREIRA, C. M. Stem cell markers expression evaluation in Oral Squamous Cell Carcinoma. Research, Society and Development, [S. l.], v. 10, n. 13, p. e314101320840, 2021. DOI: 10.33448/rsd-v10i13.20840. Disponível em: Acesso em: 19 jan. 2022.



Agrarian and Biological Sciences