Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCR
DOI:
https://doi.org/10.33448/rsd-v10i12.20923Keywords:
Parasitism; Public health; Molecular diagnosis; Fish.Abstract
Clinostomum complanatum Rudolphi, 1814 is a digenetic trematode that infects mollusks, several species of freshwater fish and piscivorous birds. In its life cycle, C. complanatum has as its second intermediate host some freshwater fish, among which Jundiá (Rhamdia quelen). Thus, the aim of the present study was to evaluate the prevalence of Clinostomum infections in Jundiás captured in the estuary region of Pelotas/RS and to standardize a Polymerase Chain Reaction (PCR) to diagnose the presence of metacercariae in tissue samples from fish captured in this estuary. In the cross-sectional study and the standardization of PCR, 174 samples of Jundiás (Rhamdia quelen), captured by amateur fishermen at 5 different points in locations close to Laguna dos Patos, Lagoa Mirim or the São Gonçalo Channel were used. The prevalence of parasitism by Clinostomum spp. with diagnosis through parasitological keys was 36.21%, with variations in prevalence according to the reported capture points of specimens from Jundiá, being confirmed by standardized PCR. It is concluded that the prevalence is high in the analyzed silver catfish and PCR can be an auxiliary tool in the diagnosis of this parasite, increasing not only the sensitivity, but also the speed of the test. Such factors will be of great importance for future epidemiological studies, as well as for public health, since the consumption of freshwater fish, typical of oriental cuisine, is expanding in Rio Grande do Sul and Brazil.
References
Costa, D. P. C., Monteiro, C. M. & Brasil-Sato, M. C. (2015). Digenea of Hoplias intermedius and Hoplias malabaricus (Actinopergygii, Erythrinide) from upper São Francisco River, Brazil. Brazilian Journal of Veterinary Parasitology, 2961, 129–135.
Dias, M. L. G. G., Minte-Vera, C. V., Eiras, J. C., Machado, M. H., Souza, G. T. R. & Pavanelli, G. C. (2006). Ecology of Clinostomum complanatum Rudolphi, 1814 (Trematoda: Clinostomidae) infecting fish from the floodplain of the high Paraná River, Brazil. Parasitology research, 99, 675-681.
Dias, J. S., Pozza, A., Pesenti, T. C., Pereira Jr, J. & Berne, M. E. A. (2016). Helmintos parasitos de Rhamdia quelen (Quoy & Gaimard, 1824) no sul do Brasil. Science and Animal Health, 4, 2-20.
Eiras, J.C., Dias, M.L.G., Pavanelli, G.C. & Machado, M.H. (1999). Histological Studies on the Effects of Clinostomum marginatum (Digenea: Clinostomidae) in its Second Intermediate Host Loricariichthys platymetopon (Osteichthyes, Loricariidae) of the Upper Paraná, Brazil. Acta Scientifica, 21, 237-241.
Hara, H., Miyauchi, Y., Tahara, S. & Yamashita, H. (2014). Human laryngitis caused by Clinostomum complanatum. Nagoya journal of medical science, 76, 181.
Lee, G. S., Park, S. W., Kim, J., Seo, K. S., You, K. W., Chung, J. H., Moon, H. C. & Hong, G. Y. (2017). A case of endoscopically treated laryngopharyngitis resulting from Clinostomum complanatum infection. Korean Journal of Gastroenterology, 69,177–180.
Lo, C. F., Huber, F., Kou, G. H. & Lo, C. J. (1981). Studies of Clinostomum complanatum (RUD., 1819). Fish Pathology, 15, 219-227.
Miller, D. L., Bursey, C. R., Gray, M. J. & Smith, L. M. (2004). Metacercariae of Clinostomum attenuatum in Ambystoma tigrinum mavortium, Bufo cognatus and Spea multiplicata from West Texas. Journal of Helminthology, 78, 373–376.
Park, C. W., Kim, J. S., Joo, H. S. & Kim, J. (2009). A human case of Clinostomum complanatum infection in Korea. The Korean journal of parasitology, 47, 401.
Pavanelli, G. C., Eiras, J. C. & Takemoto, R. M. (1998). Doenças de peixes: Profilaxia. Diagnóstico e Tratamento, 3.
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Disponível em: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1. Acesso em: 27 Setembro 2021.
Silva, A. S., Monteiro, S. G., Doyle, R. L., Pedron, F. A., Filipetto, J. E. & Radunz-Neto, J. (2008). Ocorrência de Clinostomum complanatum em diferentes espécies de peixes de uma piscicultura do Município de Santa Maria–RS. Veterinária e Zootecnia, 15, 27-32.
Simsek, E. (2018). Occurrence and molecular characterization of Clinostomum complanatum (Trematoda: Clinostomidae) in freshwater fishes caught from Turkey. Parasitology Research, 117, 2117–2124.
Song, H. B., Choi, M. H. & Chung, E. J. (2018). Human laryngeal infection by Clinostomum complanatum. The American journal of tropical medicine and hygiene, 98, 7.
Thatcher, V. E. (1981). Patologia de peixes da Amazônia Brasileira, 1. Aspectos gerais. Acta Amazonica, 11, 125-140.
Vianna, R. T., Pereira Júnior, J. & Brandão, D. A. (2005). Clinostomum complanatum (Digenea, Clinostomidae) density in Rhamdia quelen (Siluriformes, Pimelodidae) from South Brazil. Brazilian Archives of biology and Technology, 48, 635-642.
Williams, M., Hernandez-Jover, M. & Shamsi, S. (2020). Fish substitutions which may increase human health risks from zoonotic seafood borne parasites: A review. Food Control, 107429.
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Copyright (c) 2021 Iuri Vladimir Pioly Marmitt; João Pedro Mello Silva; Rodrigo Casquero Cunha; Samuel Rodrigues Felix; Flávia Aleixo Vasconcellos; Leandro Quintana Nizoli; Éverton Fagonde da Silva
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