Vesicular dermatitis caused by multirresistent Pseudomonas spp. in the carpet python (Morelia spilota Lacepede, 1804) (Squamata: Pythonidae) – case report




Bacteria; Infection; Reptiles; Snakes; Zoonosis.


Among the diseases that can affect snakes in captivity, infectious diseases are one of the main factors responsible for high morbidity and mortality rates. One of the main agents is Pseudomonas spp. that, besides being zoonotic, is generally multiresistant to several antimicrobials, representing a public health risk. This paper aims to report the occurrence of multidrug-resistant Pseudomonas sp. in M. spilota presenting vesicular dermatitis, as well as the microbiological, pathological and therapeutic description. In the care of a 7-year-old male carpet python, we noticed several vesicles containing a translucent viscous liquid, located in the skin of the entire body of the animal, in addition to some depigmented and elevated scales. Thus, material was collected from a freshly opened vesicle for bacterial culture and antibiogram. Blood was also collected for hematological and serum biochemical tests. Due to the suspicion of bacterial infection, treatment was instituted using enrofloxacin, ketoprofen, and potassium permanganate baths. After treatment, it was noticed that the vesicles had ceased, but after about ten days the animal died, suggesting systemic involvement. Hematological exams showed heterophilic leukocytosis, lymphocytosis, and monocytosis, besides an increase in AST. The results of the culture and antibiogram were available after the death of the animal, suggesting Pseudomonas spp. with resistance to several drugs. This paper describes the first report of vesicular dermatitis caused by multidrug-resistant Pseudomonas spp. in M. spilota, thus assisting in the medicine and ex situ conservation of these animals.


Branch, S., Hall, L., Blackshear, P., & Chernoff, N. (1998). Infectious dermatitis in a ball python (Python regius) colony. Journal of Zoo and Wildlife Medicine, 29, 461-464.

Breidenstein, E. B., de la Fuente-Núñez, C., & Hancock, R. E. (2011). Pseudomonas aeruginosa: all roads lead to resistance. Trends in microbiology, 19(8), 419-426.

Bryant, G. L., Fleming, P. A., Twomey, L., & Warren, K. A. (2012). Factors affecting hematology and plasma biochemistry in the southwest carpet python (Morelia spilota imbricata). Journal of Wildlife Diseases, 48(2), 282-294.

Carpenter, J. (2018). Exotic Animal Formulary. (5a ed.), Elsevier.

Colinon, C., Jocktane, D., Brothier, E., Rossolini, G. M., Cournoyer, B., & Nazaret, S. (2010). Genetic analyses of Pseudomonas aeruginosa isolated from healthy captive snakes: evidence of high inter‐and intrasite dissemination and occurrence of antibiotic resistance genes. Environmental microbiology, 12(3), 716-729.

Ebani, V. V., & Fratini, F. (2005). Bacterial zoonoses among domestic reptiles. Annali della Facoltà di Medicina veterinaria, 58, 85-91.

Ferreira, P. R. B., Oliveira, A. V. D., Laborda, S. S., Freire Jr, L. J. S., Queiroz, L. D. T., & Anunciação, A. V. M. (2012). Infecção por Morganella morganii como causa de abscesso subcutâneo em Boa constrictor em conservação ex situ. Jornal Brasileiro de Ciência Animal, 5(9), 320-334.

Ferreira, P. R. B., Curvelo, L. Q. S., Gondim, G. O., Santana, G. O., & Oliveira, A. V. D. (2014). Sinais clínicos e alterações necroscópicas em filhotes de Eunectes murinus (Linnaeus, 1758) infectados com bactérias Gram negativas multirresistentes. Jornal Brasileiro de Ciência Animal, 7(14), 508-522.

Foti, M., Giacopello, C., Fisichella, V., & Latella, G. (2013). Multidrug-resistant Pseudomonas aeruginosa isolates from captive reptiles. Journal of exotic pet medicine, 22(3), 270-274.

Jacobson, E. R., Adams, H. P., Geisbert, T. W., Tucker, S. J., Hall, B. J., & Homer, B. L. (1997). Pulmonary lesions in experimental ophidian paramyxovirus pneumonia of Aruba Island rattlesnakes, Crotalus unicolor. Veterinary Pathology, 34(5), 450-459.

Klaphake E., Gibbons P.M., Sladky K.K.& Carpenter J.W. Reptiles. In: Carpenter J.W., 5ed. Exotic Animal Formulary. St. Louis: Elsevier; 2017:83–182.

Liu, P. Y., Weng, L. L., Tseng, S. Y., Huang, C. C., Cheng, C. C., Mao, Y. C., & Tung, K. C. (2017). Colistin resistance of Pseudomonas aeruginosa isolated from snakes in Taiwan. Canadian Journal of Infectious Diseases and Medical Microbiology, 2017, 1-5.

Martins, N. B., Ferreira, L. A. R., Santos, A. L. Q., de Souza, R. R., Oliveira, W. J., de Almeida Moreira, T., Queiroz, C. L., & Lima, A. M. C. (2017). Dermatopathy caused by Enterobacter aerogenes and Pseudomonas aeruginosa in Boa constrictor amarali. Acta Scientiae Veterinariae, 45(1), 1-4.

Martins, N. B., Ferreira, L. A. R., Queiroz, C. L., Buiatte, A. B. G., Lima, A. M. C., de Souza, R. R., Oliveira, W. J. & Santos, A. L. Q. (2021). Caseous Stomatitis Caused by Pseudomonas aeruginosa in Boa constrictor amarali. Acta Scientiae Veterinariae, 49(1), 1-5.

Mitchell, M. A. (2004). Snake care and husbandry. Veterinary Clinics of North America: Exotic Animal Practice, 7, 421-446.

Romero, S. B., Čížek, A., Masaříková, M., & Knotek, Z. (2015). Choanal and cloacal aerobic bacterial flora in captive green iguanas: a comparative analysis. Acta Veterinaria Brno, 84(1), 19-24.

Rubin, J., Walker, R. D., Blickenstaff, K., Bodeis-Jones, S., & Zhao, S. (2008). Antimicrobial resistance and genetic characterization of fluoroquinolone resistance of Pseudomonas aeruginosa isolated from canine infections. Veterinary microbiology, 131(1-2), 164-172.

Sala, A., Di Ianni, F., Pelizzone, I., Bertocchi, M., Santospirito, D., Rogato, F., Flisi, S., Spadini, C., Iemmi, T., Moggia, E., Parmigiani, E., Cavirani, S., Taddei, S., & Cabassi, C. S. (2019). The prevalence of Pseudomonas aeruginosa and multidrug resistant Pseudomonas aeruginosa in healthy captive ophidian. PeerJ, 7, 1-13.

Serrapicos, E. O., Casagrande, R. A., Matushima, E. R., Merusse, J. L. B. (2005) Alterações macro e microscópicas observadas em serpentes Micrurus corallinus mantidas em biotério (Reptilia – Ophidia – Elapidae). Revista Portuguesa de Ciências Veterinárias, 100, 71-74.

Stacy, N. I., Alleman, A. R.& Sayler, K. A. (2011). Diagnostic hematology of reptiles. Clinics in laboratory medicine, 31(1), 87-108.

Stahl, S. J. (2002). Veterinary management of snake reproduction. The Veterinary Clinics of North America: Exotic Animal Practice, 5(3), 615-636.

Tallowin, O., Parker, F., O'Shea, M., Vanderduys, E., Wilson, S., Shea, G. & Hobson, R. (2017). Morelia spilota. The IUCN Red List of Threatened Species 2017: e.T62232A21649539.




How to Cite

BATISTA, A. I. V.; PEREIRA, L. M. F. .; MELO NETO, J. A. de .; SANTOS, T. Z. B. dos .; ARAÚJO, Ítalo de S. .; SILVA, J. M. S. da .; PRAZERES JÚNIOR, F. R. . Vesicular dermatitis caused by multirresistent Pseudomonas spp. in the carpet python (Morelia spilota Lacepede, 1804) (Squamata: Pythonidae) – case report. Research, Society and Development, [S. l.], v. 10, n. 13, p. e205101320966, 2021. DOI: 10.33448/rsd-v10i13.20966. Disponível em: Acesso em: 6 dec. 2021.



Health Sciences