Development of a bioactive nanoemulsion (o/w) incorporated into the essential oil of Coleus aromaticus Benth (spearmint)

Authors

DOI:

https://doi.org/10.33448/rsd-v11i2.23516

Keywords:

Larvicidal; Essential oil; Coleus aromaticus.

Abstract

This study aimed to evaluate the larvicidal activity of essential oil (EO) and nanoemulsion (O/A) of Coleus aromaticus Benth (coarse mint) leaves. The plant material obtained in this research was collected in Arari (MA) in May 2021. The hydrodistillation technique was used for EO extraction in a modified Clevenger extractor system. The total phenolic compounds were determined by the Folin-Ciocalteu method. Antioxidant activity was performed by the spectrophotometric method of elimination of hydroxyl radicals from salicylic acid. For larvicidal activity, Aedes aegypti larvae were submitted to EO solutions and nanoemulsions at concentrations of 10-100 mg L-1, where larvae mortality was evaluated and LC50 was determined by the Probit method. In the total phenolic assay, the value of 350.86 mg EAT g-1 was quantified, being a significant result for the presence of the class in the essential oil. In the antioxidant activity assay, the IC50 of 195.39 mg L-1 was obtained for the action of C. aromaticus being classified as promising. The action of the EO as larvicidal was observed and the LC50 of 3.24 mg L-1 was determined, while for nanoemulsion a higher action was observed in 1.83 mg L-1. The results obtained allowed us to conclude that the studied EO presented efficient larvicidal activity against the larvae of Aedes aegypti. Finally, we highlight the potential obtained for EO and nanoemulsion obtained from the species under study, and it is important to highlight that the nanoemulsion obtained is a bioproduct formulated from the essential oil with market potential, being important in the control and combat of cases related to Aedes aegypti.

References

Campos, M. G., Webby, R. F., Markham, K. R., Mitchell, K. A., & Da Cunha, A. P. (2003). Age-induced diminution of free radical scavenging capacity in bee pollens and the contribution of constituent flavonoids. Journal of agricultural and food chemistry, 51(3), 742-745.

Balandrin, M. F., Klocke, J. A., Wurtele, E. S., & Bollinger, W. H. (1985). Natural plant chemicals: sources of industrial and medicinal materials. Science, 228(4704), 1154-1160.

Castillo, R. A. M., & González, V. P. (1999). Plectranthus amboinicus (Lour.) Spreng. Revista Cubana de Plantas Medicinales, 3, 110-115.

Cheng, S. S., Chang, H. T., Chang, S. T., Tsai, K. H., & Chen, W. J. (2003). Bioactivity of selected plant essential oils against the yellow fever mosquito Aedes aegypti larvae. Bioresource Technology, 89(1), 99-102.

Costa, Z. G. A., Romano, A. P. M., Elkhoury, A. N. M., & Flannery, B. (2011). Evolução histórica da vigilância epidemiológica e do controle da febre amarela no Brasil. Revista Pan-Amazônica de Saúde, 2(1), 11-26.

Dias, C. N., & Moraes, D. F. C. (2014). Essential oils and their compounds as Aedes aegypti L.(Diptera: Culicidae) larvicides. Parasitology research, 113(2), 565-592.

Finney, D. J. (1952). Probit analysis: a statistical treatment of the sigmoid response curve. Cambridge university press, Cambridge.

Forattini, O. (1962). Entomologia Médica. Universidade de São Paulo. S.

Furtado, R. F., de Lima, M. G., Andrade Neto, M., Bezerra, J. N., & Silva, M. G. D. V. (2005). Atividade larvicida de óleos essenciais contra Aedes aegypti L.(Diptera: Culicidae). Neotropical Entomology, 34, 843-847.

Huang, H. T., Lin, C. C., Kuo, T. C., Chen, S. J., & Huang, R. N. (2019). Phytochemical composition and larvicidal activity of essential oils from herbal plants. Planta, 250(1), 59-68.

Kelsey, R. G., Reynolds, G. W., & Rodriguez, E. (1984). Chemistry of biologically active constitutents secreted and stored in plant glandular trichomes. Biology and chemistry of plant trichomes/edited by E. Rodriguez, PL Healey and I. Mehta.

Kubitschek-KM, A. R. J., & Zero, J. M. (2014). Development of jojoba oil (Simmondsia chinensis (Link) CK Schneid.) based nanoemulsions. Lat. Am. J. Pharm, 33(3), 459-63.

Lima, T. C. P., de Almeida, A. F., de Oliveira, E. C. P., Carrera Silva Júnior, J. O., Ribeiro Costa, R. M., Pena Matos, A., & Fonseca Gomes, M. R. (2020). Desenvolvimento de nanogel de Copaifera reticulata sobre a lesão muscular em ratos usando fonoforese. Saúde e Pesquisa, 13(1).

Mendonça SC (2016). Atividades antioxidantes, anti-Candida e inibitorias da lipoxigenase e alfa-amilase de espécies medicinais do gênero Plectranthus. Dissertação de Mestrado Universidade Federal de Lavras.

Montanari, C. A., & Bolzani, V. D. S. (2001). Planejamento racional de fármacos baseado em produtos naturais. Química Nova, 24, 105-111.

Murthy, P. S., Ramalakshmi, K., & Srinivas, P. (2009). Fungitoxic activity of Indian borage (Plectranthus amboinicus) volatiles. Food Chemistry, 114(3), 1014-1018.

Nenadis, N., Llorens, L., Koufogianni, A., Díaz, L., Font, J., Gonzalez, J. A., & Verdaguer, D. (2015). Interactive effects of UV radiation and reduced precipitation on the seasonal leaf phenolic content/composition and the antioxidant activity of naturally growing Arbutus unedo plants. Journal of Photochemistry and Photobiology B: Biology, 153, 435-444.

Passari, L. M., Scarminio, I. S., Marcheafave, G. G., & Bruns, R. E. (2019). Seasonal changes and solvent effects on fractionated functional food component yields from Mikania laevigata leaves. Food chemistry, 273, 151-158.

Pirondo, A., Coulleri, J. P., Keller, H. A., & Ferrucci, M. S. (2011). Influencia de factores externos sobre la comercialización de plantas medicinales en un medio urbano: el caso de vendedores criollos e indígena en Corrientes, Argentina.

Pereira, A. P. M., Martins, T. G. T., Arruda, M. O., Dias, A. A. S., da Silva Souto, L. A., de Araújo Neto, A. P., ... & Everton, G. O. (2021). Chemical profile and larvicidal activity of essential oil obtained from the leaves of Plectranthus amboinicus (Lour.) Spreng. Research, Society and Development, 10(4), e15410413846-e15410413846.

Raju, V. D., Kishore, P. S., & Yamini, K. (2018). Experimental studies on four stroke diesel engine fuelled with tamarind seed oil as potential alternate fuel for sustainable green environment. European Journal of Sustainable Development Research, 2(1), 10.

Rodrigues, E. D. C., Ferreira, A. M., Vilhena, J. C., Almeida, F. B., Cruz, R. A., Florentino, A. C., ... & Fernandes, C. P. (2014). Development of a larvicidal nanoemulsion with Copaiba (Copaifera duckei) oleoresin. Revista Brasileira de Farmacognosia, 24, 699-705.

Santos, A. B. D. S., Everton, G. O., Júnior, R. G. D. O. C., Rosa, P. V. S., Pereira, A. P. M., dos Santos Souza, L., ... & Mouchrek Filho, V. E. (2020). Óleos essenciais de Cinnamomum zeylanicum Blume e Plectranthus amboinicus (lour.) Spreng como agentes larvicidas frente as larvas do Aedes aegypti. Brazilian Journal of Development, 6(4), 22355-22369.

Sandes, A. R. R., & Di Blasi, G. (2000). Biodiversidade e diversidade química e genética. Biotecnologia, Ciência e Desenvolvimento, 13, 28-32.

Simas, N. K., Lima, E. D. C., Conceição, S. D. R., Kuster, R. M., Oliveira Filho, A. M. D., & Lage, C. L. S. (2004). Produtos naturais para o controle da transmissão da dengue: atividade larvicida de Myroxylon balsamum (óleo vermelho) e de terpenóides e fenilpropanóides. Química nova, 27, 46-49.

Singh, G., Singh, O. P., Prasad, Y. R., De Lampasona, M. P., & Catalan, C. (2002). Studies on essential oils, Part 33: chemical and insecticidal investigations on leaf oil of Coleus amboinicus Lour. Flavour and fragrance journal, 17(6), 440-442.

Shafiq, S., Shakeel, F., Talegaonkar, S., Ahmad, F. J., Khar, R. K., & Ali, M. (2007). Development and bioavailability assessment of ramipril nanoemulsion formulation. European journal of pharmaceutics and biopharmaceutics, 66(2), 227-243.

Senthilkumar, A., & Venkatesalu, V. (2010). Chemical composition and larvicidal activity of the essential oil of Plectranthus amboinicus (Lour.) Spreng against Anopheles stephensi: a malarial vector mosquito. Parasitology research, 107(5), 1275-1278.

Singh, H. P., Kaur, S., Negi, K., Kumari, S., Saini, V., Batish, D. R., & Kohli, R. K. (2012). Assessment of in vitro antioxidant activity of essential oil of Eucalyptus citriodora (lemon-scented Eucalypt; Myrtaceae) and its major constituents. LWT-Food science and Technology, 48(2), 237-241.

Sminorff, N., Cumbes, Q. J. (1989). Atividade sequestrante de radical hidroxila de solutos compatíveis. Phytochemistry, 28(4), 1057-1060.

Silva, W. J. D. (2006). Atividade larvicida do óleo essencial de plantas existentes no estado de Sergipe contra Aedes aegypti Linn.

Silva, H. H. G. D., Silva, I. G. D., Elias, C. N., Lemos, S. P. S., & Rocha, A. P. (1995). Idade fisiológica dos ovos de aedes (stegomyia) aegypti (Linnaeus, 1762)(diptera, culicidae).

Sousa, C. M. D. M., Silva, H. R., Ayres, M. C. C., Costa, C. L. S. D., Araújo, D. S., Cavalcante, L. C. D., ... & Chaves, M. H. (2007). Total phenolics and antioxidant activity of five medicinal plants. Quimica Nova, 30(2), 351-355.

Sugumar, S., Clarke, S. K., Nirmala, M. J., Tyagi, B. K., Mukherjee, A., & Chandrasekaran, N. (2014). Nanoemulsion of eucalyptus oil and its larvicidal activity against Culex quinquefasciatus. Bulletin of entomological research, 104(3), 393-402.

Sundararajan, R., & Koduru, R. (2016). In vitro antioxidant activity on roots of Limnophila heterophylla. Free Radicals and Antioxidants, 6(2), 178-185.

Taveira, L. A., Fontes, L. R., Natal, D., & Gomes, A. D. C. (2001). Manual de diretrizes e procedimentos no controle do Aedes aegypti. In Manual de diretrizes e procedimentos no controle do Aedes aegypti (pp. 108-108).

Terto, M. V. C., Gomes, J. M., Araújo, D. I. A., Silva, T. S., Ferreira, J. M., Souza, J. J. N., ... & Tavares, J. F. (2020). Photoprotective activity of Plectranthus amboinicus extracts and HPLC quantification of rosmarinic acid. Revista Brasileira de Farmacognosia, 1-6.

Viegas Jr, C., Bolzani, V. D. S., & Barreiro, E. J. (2006). Os produtos naturais e a química medicinal moderna. Química nova, 29, 326-337.

Waterhouse, A. L. (2002). Determination of total phenolics. Current protocols in food analytical chemistry, 6(1), I1-1.

Published

22/01/2022

How to Cite

FERREIRA, M. L. .; OLIVEIRA, J. P. M. .; LIMA, T. P. de .; SOUSA, B. A. de S. de .; ALMEIDA, R. de A.; SEREJO, A. P. M. .; MARINHO, S. C. .; OLIVEIRA, A. C. da S. de .; GOMES, P. R. B.; MOUCHREK FILHO, V. E. .; EVERTON, G. O. . Development of a bioactive nanoemulsion (o/w) incorporated into the essential oil of Coleus aromaticus Benth (spearmint). Research, Society and Development, [S. l.], v. 11, n. 2, p. e18711223516, 2022. DOI: 10.33448/rsd-v11i2.23516. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/23516. Acesso em: 22 nov. 2024.

Issue

Section

Health Sciences