Chlorhexidine added to different adhesive systems - Assessment of antibacterial activity and bond strength

Authors

DOI:

https://doi.org/10.33448/rsd-v11i3.25785

Keywords:

Chlorhexidine; Dentin-bonding agents; Anti-bacterial agents.

Abstract

This study aimed to assess the effects of adding chlorhexidine (CHX) to different adhesive systems on antibacterial activity and bond strength after 24 hours and 30 days under in vitro conditions. A total of 210 bovine incisor crowns and divided into three groups, based on the adhesive system: Single Bond Plus (SB), Clearfil SE Bond (CL), and AdheSE Primer and Bond (AD). All groups were subdivided by CHX concentration (n=7): no CHX, CHX applied before the adhesive (CPA), and the adhesive incorporated with 0.5% CHX, 1.0% CHX, and 2.0% CHX. The micro shear test was conducted on one-half of the specimens after 24 hours, and on the other half after 30 days. Antibacterial activity was evaluated using the agar diffusion test with Streptococcus mutans. The bond strength variable was assessed using the factorial analysis of variance for repeated measurements, whereas the variable of the bacterial halo size was subjected to the Kruskal–Wallis nonparametric test. The significance level was 5% for both tests. The qualitative variable of fracture pattern was assessed by descriptive statistical analysis. Thus, CHX concentration of 2% (10.80 ±2.83) and CPA (9.95 ±1.47) (p >0.05) helped to increase the bond strength values for most adhesive systems, whereas CL had the highest bond strength values. The CL adhesive system also presented the best results in the antibacterial test with S.mutans.

References

André, C. B., Gomes, B. P., Duque, T. M., Stipp, R. N., Chan, D. C. & Ambrosano, G. M. (2015). Dentine bond strength and antimicrobial activity evaluation of adhesive systems. Journal of Dentistry, 43(4), 466-75.

Araújo, M. S., Souza, L. C., Apolonio, F. M., Barros, L. O., Reis, A. & Loguercio, A. D. (2015) Two-year clinical evaluation of chlorhexidine incorporation in two-step self-etch adhesive. Journal of Dentistry, 43(1),140-8.

Breschi, L., Mazzoni, A., Nato, F., Carrilho, M., Visintini, E. & Tjäderhane, L. (2010). Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study. Dental Materials, 26(4), 320-5.

Breschi, L., Mazzoni, A., Ruggeri, A., Cadenaro, M., Di Lenarda, R. & De Stefano, E. (2008). Dental adhesion review: aging and stability of the bonded interface. Dental Materials, 24(1), 90-101.

Carrilho, M. R., Carvalho, R. M., de Goes, M. F., di Hipólito, V., Geraldeli, S., & Tay, F. R. (2007). Chlorhexidine preserves dentin bond in vitro. Journal of Dental Research, 86(1), 90-4.

Munck, J., Mine, A., Van den Steen, P. E., Van Landuyt, K. L., Poitevin, A. & Opdenakker, G. (2010). Enzymatic degradation of adhesive-dentin interfaces produced by mild self-etch adhesives. European Journal of Oral Science, 118(5), 494-501.

Gondim, J.O., Duque, C., Hebling, J. & Giro, E.M. (2008). Influence of human dentine on the antibacterial activity of self-etching adhesive systems against cariogenic bacteria. Journal of Dentistry, 36(4), 241-8.

Gunaydin, Z., Yazici, A.R., Cehreli, Z.C. (2016). In vivo and in vitro effects of chlorhexidine pretreatment on immediate and aged dentin bond strengths. Operative Dentistry, 41(3), 258-67.

Hajizadeh, H., Ghavamnasiri, M. & Majidinia, S. (2013). Randomized clinical evaluation of the effect of chlorhexidine on postoperative sensitivity of posterior composite resin restorations. Quintessence International, 44(10), 793-8.

Hashimoto, M., Hirose, N., Kitagawa, H., Yamaguchi, S., Imazato, S. (2018). Improving the durability of resin-dentin bonds with an antibacterial monomer MDPB. Dental Materials Journal, 37(4),620-7.

Kim, S.R. & Shin, D.H. (2014) Antibacterial effect of self-etching adhesive systems on Streptococcus mutans. Restorative Dentistry & Endodontics, 39(1), 32-8.

Li, H., Li, T., Li, X., Zhang, Z., Li, P. & Li, Z. (2015). Morphological effects of MMPs inhibitors on the dentin bonding. International Journal of Clinical and Experimental Medicine, 8(7),10793-803.

Montagner, A.F., Sarkis-Onofre, R., Pereira-Cenci, T. & Cenci, M.S. (2014). MMP inhibitors on dentin stability: a systematic review and meta-analysis. Journal of Dental Research, 93(8), 733-43.

Osorio, R., Pisani-Proenca, J., Erhardt, M.C., Osorio, E., Aguilera, F.S. & Tay, F.R. (2008). Resistance of ten contemporary adhesives to resin-dentine bond degradation. Journal of Dentistry, 36(2), 163-9.

Ou, Q., Hu, Y., Yao, S., Wang, Y. & Lin, X. (2018). Effect of matrix metalloproteinase 8 inhibitor on resin-dentin bonds. Dental Materials, 34(5), 756-63.

Penmetsa, R.K., Rekha, A.S., Poppuri, K.C., Prashanth, P.S. & Garapati S. (2014). An in vitro evaluation of antibacterial properties of self-etching dental adhesive systems. Journal of Clinical and Diagnostic Research, 8(7), 1-5.

Perote, L.C., Kamozaki, M.B., Gutierrez, N.C., Tay, F.R. & Pucci, C.R. Effect of matrix metalloproteinase-inhibiting solutions and aging methods on dentin bond strength. Journal of Adhesive Dentistry, 17(4), 347-52.

Ricci, H.A., Sanabe, M.E., Costa, C.A. & Hebling, J (2010). Effect of chlorhexidine on bond strength of two-step etch-and-rinse adhesive systems to dentin of primary and permanent teeth. American Journal of Dentisty, 23(3), 128-32.

Rocha, R., Soares, F.Z., Rodrigues, C.R. & Rodrigues Filho, L.E. (2007). Influence of aging treatments on microtensile bond strength of adhesive systems to primary dentin. Journal of Dentistry for Children, 74(2), 109-12.

Sano, H., Shono, T., Sonoda, H., Takatsu, T., Ciucchi, B. & Carvalho, R.M. (1994). Relation between surface area for adhesion and tensile bond strength evaluation of a micro-tensile bond test. Dental Materials, 10(4), 236-40.

Stanislawczuk, R., Pereira, F., Muñoz, M.A., Luque, I., Farago, P.V. & Reis, A. (2014). Effects of chlorhexidine-containing adhesives on the durability of resin-dentine interfaces. Journal of Dentistry, 42(1), 39-47.

Strobel, S. & Hellwig, E. (2015). The effects of matrix-metallo-proteinases and chlorhexidine on the adhesive bond. Swiss Dental Journal, 125(2),134-45.

Suma, N.K., Shashibhushan, K.K. & Reddy, V.V.S. (2017). Effect of dentin disinfection with 2% chlorhexidine gluconate and 0.3% iodine on dentin bond strength: an in vitro study. International Journal of Clinical Pediatric Dentistry, 10(3), 223-8.

Tekçe, N., Tuncer, S., Demirci, M. & Balci S. (2016). Do matrix metalloproteinase inhibitors improve the bond durability of universal dental adhesives? Scanning, 238(6), 535-44.

Van Landuyt, K.L., Kanumilli, P., De Munick, J., Peumans, M., Lambrechts, P. & Van Meerbeek, B. (2006). Bond strength of a mild self-etch adhesive with and without prior acid-etching. Journal of Dentistry, 34(1), 77-85.

Downloads

Published

18/02/2022

How to Cite

LIMA, B. C. de O.; ZENI, T. C. .; MENDONÇA, M. J. .; ROQUE, J. V. O.; UEDA, J. K.; MENOLLI, R. A.; CAMILOTTI, V. Chlorhexidine added to different adhesive systems - Assessment of antibacterial activity and bond strength. Research, Society and Development, [S. l.], v. 11, n. 3, p. e19011325785, 2022. DOI: 10.33448/rsd-v11i3.25785. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/25785. Acesso em: 20 apr. 2024.

Issue

Section

Health Sciences